Microsatellite instability typing in serum and tissue of patients with colorectal cancer: comparing real time PCR with hybridization probe and high-performance liquid chromatography

Microsatellite instability typing in serum and tissue of patients with colorectal cancer: comparing real time PCR with hybridization probe and high-performance liquid chromatography

Allelic variation of BAT-25 (a 25-repeat quasimonomorphic poly T) and BAT-26 (a 26-repeat quasimonomorphic polyA) loci as two mononucleotide microsatellite markers, were analyzed with high-performance liquid chromatography (HPLC) compared with Real-Time PCR using hybridization probes. BAT-26 and BAT...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports Vol. 41; no. 5; pp. 2835 - 2844
Main Authors: Mokarram, P., Rismanchi, M., Alizadeh Naeeni, M., Mirab Samiee, S., Paryan, M., Alipour, A., Honardar, Z., Kavousipour, S., Naghibalhossaini, F., Mostafavi-Pour, Z., Monabati, A., Hosseni, S. V., Shamsdin, S. A.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-05-2014
Springer Nature B.V
Subjects:
Adult
Aged
Animal Anatomy
Animal Biochemistry
Biomarkers, Tumor
Biomedical and Life Sciences
Case-Control Studies
Cell Line, Tumor
Chromatography, High Pressure Liquid
Colorectal cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Female
Genetic Markers
Germ-Line Mutation
Histology
Humans
Life Sciences
Male
Microsatellite Instability
Microsatellite Repeats
Middle Aged
Morphology
Neoplasm Grading
Neoplasm Staging
Nucleic Acid Hybridization
Polymorphism
Real-Time Polymerase Chain Reaction
ROC Curve
BAT-25
Microsatellite Instability
Real time PCR
BAT-26
HPLC
Colorectal cancer
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Allelic variation of BAT-25 (a 25-repeat quasimonomorphic poly T) and BAT-26 (a 26-repeat quasimonomorphic polyA) loci as two mononucleotide microsatellite markers, were analyzed with high-performance liquid chromatography (HPLC) compared with Real-Time PCR using hybridization probes. BAT-26 and BAT-25 markers were used to determine an appropriate screening technique with high sensitivity and specificity to diagnose microsatellite instability (MSI) status in patients with colorectal cancer (CRC). One of the pathways in colorectal tumor genesis is microsatellite instability (MSI + ). MSI is detected in about 15 % of all CRCs; 3 % are of these are associated with Lynch syndrome and the other 12 % are caused by sporadic. Colorectal tumors with MSI have distinctive features compared with microsatellite stable tumors. Due to the high percentage of MSI + CRC in Iran, screening of this type of CRC is imperative. Two markers were analyzed in tissues and sera of 44 normal volunteers and tumor and matched normal mucosal tissues as well as sera of 44 patients with sporadic CRC. The sensitivity and specificity of BAT-26 with real time PCR method (Hybridization probe) were 100 % in comparison with sequencing method as the gold standard, while HPLC had a lower sensitivity and specificity. According to HPLC data, BAT-26 was more sensitive than BAT-25 in identifying MSI tumors. Therefore, MSI typing using the BAT-26 hybridization probe method compared to HPLC could be considered as an accurate method for diagnosing MSI in CRC tumors but not in serum circulating DNAs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-014-3138-1