Search Results - "Hosea, N A"

Twenty years of biochemistry of human P450S : Purification, expression, mechanism, and relevance to drugs by GUENGERICH, F. P, HOSEA, N. A, PARIKH, A, BELL-PARIKH, L. C, JOHNSON, W. W, GILLAM, E. M. J, SHIMADA, T

“…Today cytochrome P450 (P450) research is accepted as an integral part of drug development and discovery. Work leading to this point included biochemical…”
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Structural bases for the specificity of cholinesterase catalysis and inhibition by Taylor, Palmer, Radic, Zoran, Hosea, Natilie A., Camp, Shelley, Marchot, Pascale, Berman, Harvey A.

“…The availability of a crystal structure and comparative sequences of the cholinesterases has provided templates suitable for analyzing the molecular bases of…”
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Elucidation of Distinct Ligand Binding Sites for Cytochrome P450 3A4 by Hosea, Natilie A, Miller, Grover P, Guengerich, F. Peter

“…Cytochrome P450 (P450) 3A4 is the most abundant human P450 enzyme and has broad selectivity for substrates. The enzyme can show marked catalytic…”
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Specificity and Orientation of Trigonal Carboxyl Esters and Tetrahedral Alkylphosphonyl Esters in Cholinesterases by Hosea, Natilie A, Berman, Harvey A, Taylor, Palmer

“…We have examined the specificity of planar carboxyl and tetrahedral phosphonyl esters for mouse cholinesterases and have delineated the orientation of these…”
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Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride by Kraut, D, Goff, H, Pai, R K, Hosea, N A, Silman, I, Sussman, J L, Taylor, P, Voet, J G

“…Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl…”
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Aspartate 74 as a Primary Determinant in Acetylcholinesterase Governing Specificity to Cationic Organophosphonates by Hosea, Natilie A, Radić, Zoran, Tsigelny, Igor, Berman, Harvey Alan, Quinn, Daniel M, Taylor, Palmer

“…Through site-specific mutagenesis, we examined the determinants on acetylcholinesterase which govern the specificity and reactivity of three classes of…”
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Oxidation of Nonionic Detergents by Cytochrome P450 Enzymes by Hosea, Natilie A., Guengerich, F.Peter

“…Nonionic phenolic detergents are commonly used in the purification of membrane-associated proteins. Triton N-101 was shown to be oxidized by NADPH-fortified…”
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Analysis of cholinesterase inactivation and reactivation by systematic structural modification and enantiomeric selectivity by Taylor, Palmer, Wong, Lilly, Radić, Zoran, Tsigelny, Igor, Brüggemann, Roger, Hosea, Natilie A., Berman, Harvey A.

“…We show here with a congeneric series of Rp- and Sp-alkoxymethyl phosphonothiolates of known absolute stereochemistry that chiral selectivity in their reaction…”
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Purification of cytochromes P450: products of bacterial recombinant expression systems by Guengerich, F P, Hosea, N A, Martin, M V

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Determining ligand orientation and transphosphonylation mechanisms on acetylcholinesterase by Rp, Sp enantiomer selectivity and site-specific mutagenesis by Taylor, P, Hosea, N A, Tsigelny, I, Radić, Z, Berman, H A

“…Acetylcholinesterase, an enzyme of the serine hydrolase family, catalyzes the rapid hydrolysis of certain carboxyl esters. Other acyl esters efficiently…”
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Reconstitution premixes for assays using purified recombinant human cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b5 by Shaw, P M, Hosea, N A, Thompson, D V, Lenius, J M, Guengerich, F P

“…The development of enzyme and buffer premixes for in vitro biotransformation assays is described. The protein premixes contain a mixture of three recombinant…”
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Factors affecting the clinical development of cytochrome P450 3A substrates by GIBBS, Megan A, HOSEA, Natilie A

“…The objective of this review is to evaluate the risks associated with the discovery and development of cytochrome p450 (CYP) 3A substrates. CYP3A is the most…”
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Purification of cytochrome P-450 enzymes by Bell-Parikh, L C, Hosea, N A, Martin, M V, Guengerich, F P

“…Among the liver P-450 xenobiotic-metabolizing enzymes, P450-2E1 is of interest because of its activation of potent carcinogens, and P-450 1A2 is of interest…”
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