Search Results - "Horn, Anton"

Composition analysis of detergents of the polyoxyethylene type: comparison of thin-layer chromatography, reversed-phase chromatography and matrix-assisted laser desorption/ionization mass spectrometry by Arnim Cumme, Gerhard, Blume, Eva, Bublitz, Renate, Hoppe, Horst, Horn, Anton

“…Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can be used to determine the distribution of single polymer species of non-ionic…”
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Structural study on the carbohydrate moiety of calf intestinal alkaline phosphatase by Bublitz, Renate, Hoppe, Horst, Cumme, Gerhard A., Thiele, Mariana, Attey, Adrian, Horn, Anton

“…Surprisingly alkaline phosphatase (AP) (EC 3.1.3.1) of calf intestine is found in large amounts, e.g. 80%, within chyme. Most of the enzyme is present as a…”
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Glycosylphosphatidylinositol-specific phospholipase D in blood serum by Rhode, Heidrun, Lopatta, Eric, Schulze, Margarete, Pascual, Carlos, Schulze, Hans-Peter, Schubert, Klaus, Schubert, Harald, Reinhart, Konrad, Horn, Anton

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Microheterogeneity of the hydrophobic and hydrophilic part of the glycosylphosphatidylinositol anchor of alkaline phosphatase from calf intestine by Armesto, J, Hannappel, E, Leopold, K, Fischer, W, Bublitz, R, Langer, L, Cumme, G.A, Horn, A

“…Digestion of calf intestine alkaline phosphatase with pronase and subsequent dephosphorylation of the released peptidyl-(Etn-P)2-glycosyl-PtdIns with HF…”
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UV measurements in microplates suitable for high-throughput protein determination by Kreusch, Stefan, Schwedler, Sina, Tautkus, Bärbel, Cumme, Gerhard A, Horn, Anton

“…An UV spectrophotometric method for protein determination using microplates is described. Using the SPECTRAmax PLUS reader, the UVStar 96- and 384-well…”
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Multidimensional proteomics of human serum using parallel chromatography of native constituents and microplate technology by Horn, Anton, Kreusch, Stefan, Bublitz, Renate, Hoppe, Horst, Cumme, Gerhard A., Schulze, Margarete, Moore, Thomas, Ditze, Günter, Rhode, Heidrun

“…A versatile, multidimensional, and non‐denaturing proteome separation procedure using microplate technology is presented, yielding a digitized image of…”
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An improved method for checking HTS/uHTS liquid-handling systems by Rhode, Heidrun, Schulze, Margarete, Renard, Simon, Zimmermann, Peter, Moore, Thomas, Cumme, Gerhard A, Horn, Anton

“…An efficient method is presented to determine precision and accuracy of multichannel liquid-handling systems under conditions near to application. The method…”
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Convex corner undercutting of silicon in anisotropic KOH etching: the new step-flow model of 3-D structuring and first simulation results by Schroder, H., Obermeier, E., Horn, A., Wachutka, G.K.M.

“…In this paper, the mechanism of convex corner (CC) undercutting of Si/sub {100}/ in pure aqueous KOH solutions is revisited by proposing the step-flow model of…”
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Biotinidase determination in serum and dried blood spots—high sensitivity fluorimetric ultramicro-assay by Broda, Elke, Baumgartner, E.Regula, Scholl, Sabine, Stopsack, Marina, Horn, Anton, Rhode, Heidrun

“…A miniaturized quantitative biotinidase assay has been developed using biotin 6-amidoquinoline as substrate and the 100-fold enhanced fluorescence of…”
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Glycosylphosphatidylinositol-specific phospholipase D in blood serum:: Is the liver the only source of the enzyme? by Rhode, Heidrun, Lopatta, Eric, Schulze, Margarete, Pascual, Carlos, Schulze, Hans-Peter, Schubert, Klaus, Schubert, Harald, Reinhart, Konrad, Horn, Anton

“…In cases of systemic inflammatory response syndrome, sepsis, and septic shock, the activity of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD)…”
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Glycosylphosphatidylinositol-specific phospholipase D of human serum--activity modulation by naturally occurring amphiphiles by Rhode, H, Schulze, M, Cumme, G A, Göhlert, A, Blume, E, Bublitz, R, Schilling, K, Horn, A

“…The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was…”
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Determination, purification, and characterization of alpha-NADH and alpha-NADPH by Klemm, A, Steiner, T, Flötgen, U, Cumme, G A, Horn, A

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Gel chromatographic characterization of the hydrophobic interaction of glycosylphosphatidylinositol-alkaline phosphatase with detergents by Gehrhardt, S, Blume, E, Cumme, G A, Bublitz, R, Rhode, H, Horn, A

“…The interaction of a glycosylphosphatidylinositol (GPI) protein with different detergents was studied for the first time with a purified protein. Four…”
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Realistic step flow model for orientation-dependent wet etching implemented in a modular TCAD environment by HORN, Anton, WITTMANN, Franz, WACHUTKA, Gerhard

“…In this paper we present a new simulation tool for orientation-dependent wet etching of silicon which is based on a new model proposed by Schroder. What is…”
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Glycosylphosphatidylinositol-Alkaline Phosphatase from Calf Intestine as Substrate for Glycosylphosphatidylinositol-Specific Phospholipases - Microassay Using Hydrophobic Chromatography in Pipet Tips by Rhode, H., Hoffmannblume, E., Schilling, K., Gehrhardt, S., Gohlert, A., Buttner, A., Bublitz, R., Cumme, G.A., Horn, A.

“…An electrophoretically homogeneous glycosylphosphatidylinositol-alkaline phosphatase fraction from calf intestine, obtained by hydrophobic chromatography, was…”
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Multiwavelength photometry of thermochromic indicator solutions for temperature determination in multicuvettes by Schilling, K, Cumme, G A, Hoffmann-Blume, E, Hoppe, H, Horn, A

“…If buffer/indicator systems are used as optical thermometers in multicuvettes, the temperature resolution is limited by errors of optical measurements produced…”
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Characterization of the interaction of alkaline phosphatase with an activity inhibiting monoclonal antibody by progress curve analysis by Cumme, Gerhard A, Walter, Uwe, Bublitz, Renate, Hoppe, Horst, Rhode, Heidrun, Horn, Anton

“…Using the enzyme activity inhibiting monoclonal antibody IB 10B8 against alkaline phosphatase of calf intestine (AP), the interaction of a macromolecular…”
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A new graphical method for determining the affinity constants of monoclonal antibodies to enzymes by Ehle, H, Gödicke, C, Horn, A

“…A new graphical method is presented for determining the affinity constants of antibodies to enzymes. The method does not require purification of reactants or…”
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Heterogeneity of glycosylphosphatidylinositol‐anchored alkaline phosphatase of calf intestine by BUBLITZ, Renate, ARMESTO, Julio, HOFFMANN‐BLUME, Eva, SCHULZE, Margarete, RHODE, Heidrun, HORN, Anton, AULWURM, Steffen, HANNAPPEL, Ewald, FISCHER, Werner

“…A method is described for large‐scale purification of glycosylphosphatidylinositol‐anchored alkaline phosphatase from intestinal mucosa and chyme to…”
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Determination of rate constants of monoclonal antibodies to enzymes by Cumme, G A, Bublitz, R, Ehle, H, Horn, A

“…A novel graphical method for determining rate constants of the immune reaction of enzyme-inhibiting or -activating antibodies has been evaluated. The…”
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