Search Results - "Horiuti, Yoshifumi"

Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose by Imamura, S, Horiuti, Y

“…Phospholipase D [phosphatidylcholine cholinehydrolase, EC 3.1.4.4] excreted from Streptomyces chromofuscus was purified from the culture supernatant by…”
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Quantification of Cell Nuclei Isolated from Hepatocytes by Cell Lysis with Nonionic Detergent in Citric Acid by Horiuti, Yoshifumi, Ogishima, Motohiro, Yano, Kazuo, Shibuya, Yuzo

“…A method was developed for determining the number of nuclei of hepatocytes cultured on collagen gel using a nonionic detergent, Nikkol BO-10TX. The cells were…”
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Purification and characterization of choline oxidase from Arthrobacter globiformis by Ikuta, S, Imamura, S, Misaki, H, Horiuti, Y

“…Choline oxidase was purified from the cells of Arthrobacter globiformis by fractionations with acetone and ammonium sulfate, and column chromatographies on…”
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Hormonal Regulations of Alkaline Phosphatase, 5'-Nucleotidase and γ-Glutamyltransferase Activities in Adult Rat Hepatocytes Cultured in Serum-Free Medium Collagen Gel by Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo

“…Primary hepatocytes were cultured on collagen gel in serum-free, α-modified Eagle's minimum essential medium containing 0.1 μMinsulin, 0.1 μM dexamethasone, 10…”
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Effect of Calcium Concentration on Survival, Proliferation and Activities of Alkaline Phosphatase, 5'-Nucleotidase, .GAMMA.-Glutamyltransferase and Lactate Dehydrogenase of Adult Rat Hepatocytes Cultured by Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo

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Enzymatic determination of phospholipase D activity with choline oxidase by Imamura, S, Horiuti, Y

“…A new enzymatic method was developed for the assay of phospholipase D [phosphatidylcholine phosphatidohydrolase EC 3.1.4.4] from cabbage leaves using choline…”
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Role of serum in maintenance of functional hepatocytes in primary culture by Horiuti, Y, Nakamura, T, Ichihara, A

“…Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and…”
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Stimulation of Chromobacterium lipase activity and prevention of its adsorption to palmitoyl cellulose by hydrophobic binding of fatty acids by Horiuti, Y, Imamura, S

“…Fatty acids prevented adsorption of purified Chromobacterium lipase [triacylglycerol acylhydrolase, EC 3.1.1.3] onto palmitoyl cellulose (Pal-C) and also…”
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Hormonal Regulations of Alkaline Phosphatase, 5'-Nucleotidase and [gamma]-Glutamyltransferase Activities in Adult Rat Hepatocytes Cultured in Serum-Free Medium Collagen Gel by Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo

“…Primary hepatocytes were cultured on collagen gel in serum-free, α-modified Eagle's minimum essential medium containing 0.1 μMinsulin, 0.1 μM dexamethasone, 10…”
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Effect of Calcium Concentration on Survival, Proliferation and Activities of Alkaline Phosphatase, 5'-Nucleotidase, [gamma]-Glutamyltransferase and Lactate Dehydrogenase of Adult Rat Hepatocytes Cultured in Serum-free Medium by Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo

“…Mature rat hepatocytes were cultured on collagen coated dishes in serum-free a-modified Eagle's minimum essential medium containing 0.1 μM insulin, 0.1 μM…”
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Effect of Calcium Concentration on Survival, Proliferation and Activities of Alkaline Phosphatase, 5'-Nucleotidase, γ-Glutamyltransferase and Lactate Dehydrogenase of Adult Rat Hepatocytes Cultured in Serum-free Medium by Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo

“…Mature rat hepatocytes were cultured on collagen coated dishes in serum-free a-modified Eagle's minimum essential medium containing 0.1 μM insulin, 0.1 μM…”
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Purification of lipase from Chromobacterium viscosum by chromatography on palmitoyl cellulose by Horiuti, Y, Imamura, S

“…Palmitoyl cellulose was used to adsorb the extracellular lipase [triacylglycerol acyl-hydrolase EC 3.1.1.3] of Chromobacterium viscosum from crude enzyme…”
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Effective method for activity assay of lipase from Chromobacterium viscosum by Horiuti, Y, Koga, H, Gocho, S

“…A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture…”
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