Mitochondrial nanomotion measured by optical microscopy

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also f...

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Bibliographic Details
Published in:	Frontiers in microbiology Vol. 14; p. 1133773
Main Authors:	Parmar, Priyanka, Villalba, Maria Ines, Horii Huber, Alexandre Seiji, Kalauzi, Aleksandar, Bartolić, Dragana, Radotić, Ksenija, Willaert, Ronnie Guy, MacFabe, Derrick F, Kasas, Sandor
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 23-03-2023
Subjects:	metabolic substrates Microbiology mitochondria optical nanomotion rotenone short chain fatty acids rotenone optical nanomotion metabolic substrates mitochondria short chain fatty acids
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Summary:	Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Matthew Cabeen, Oklahoma State University, United States; Mar Alvarez, Institute of Microelectronics of Barcelona (CSIC), Spain; Ferran Pujol-Vila, National Microelectronics Center (CSIC), Spain Edited by: Vijay Pancholi, The Ohio State University, United States This article was submitted to Microbial Physiology and Metabolism, a section of the journal Frontiers in Microbiology These authors have contributed equally to this work and share first authorship These authors have contributed equally to this work and share last authorship
ISSN:	1664-302X 1664-302X
DOI:	10.3389/fmicb.2023.1133773