Search Results - "Hopper, T."

The role of interfacial lipids in stabilizing membrane protein oligomers by Gupta, Kallol, Donlan, Joseph A. C., Hopper, Jonathan T. S., Uzdavinys, Povilas, Landreh, Michael, Struwe, Weston B., Drew, David, Baldwin, Andrew J., Stansfeld, Phillip J., Robinson, Carol V.

“…Membrane lipids such as cardiolipin act as molecular glue to preserve the oligomeric states of membrane proteins with low oligomeric stability…”
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Mass spectrometry of intact membrane protein complexes by Laganowsky, Arthur, Reading, Eamonn, Hopper, Jonathan T S, Robinson, Carol V

“…Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful technique to study the stoichiometry, structure-function and dynamics of…”
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Collision Induced Unfolding of Protein Ions in the Gas Phase Studied by Ion Mobility-Mass Spectrometry: The Effect of Ligand Binding on Conformational Stability by Hopper, Jonathan T.S., Oldham, Neil J.

“…Ion mobility spectrometry, with subsequent mass spectrometric detection, has been employed to study the stability of compact protein conformations of…”
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Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen-deuterium exchange mass spectrometry by Toporowska, Joanna, Kapoor, Parth, Musgaard, Maria, Gherbi, Karolina, Sengmany, Kathy, Qu, Feng, Soave, Mark, Yen, Hsin-Yung, Hansen, Kjetil, Jazayeri, Ali, Hopper, Jonathan T. S., Politis, Argyris

“…G Protein Coupled Receptors (GPCRs) constitute the largest family of signalling proteins responsible for translating extracellular stimuli into intracellular…”
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High-resolution mass spectrometry of small molecules bound to membrane proteins by Gault, Joseph, Donlan, Joseph A C, Liko, Idlir, Hopper, Jonathan T S, Gupta, Kallol, Housden, Nicholas G, Struwe, Weston B, Marty, Michael T, Mize, Todd, Bechara, Cherine, Zhu, Ya, Wu, Beili, Kleanthous, Colin, Belov, Mikhail, Damoc, Eugen, Makarov, Alexander, Robinson, Carol V

“…A high-resolution, Orbitrap-based, native mass spectrometry approach allows the direct characterization of lipid, peptide and drug binding to intact membrane…”
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Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor by Yen, Hsin-Yung, Liko, Idlir, Song, Wanling, Kapoor, Parth, Almeida, Fernando, Toporowska, Joanna, Gherbi, Karolina, Hopper, Jonathan T. S., Charlton, Steven J., Politis, Argyris, Sansom, Mark S. P., Jazayeri, Ali, Robinson, Carol V.

“…G-protein-coupled receptors signal through cognate G proteins. Despite the widespread importance of these receptors, their regulatory mechanisms for G-protein…”
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Cotranslational protein assembly imposes evolutionary constraints on homomeric proteins by Natan, Eviatar, Endoh, Tamaki, Haim-Vilmovsky, Liora, Flock, Tilman, Chalancon, Guilhem, Hopper, Jonathan T. S., Kintses, Bálint, Horvath, Peter, Daruka, Lejla, Fekete, Gergely, Pál, Csaba, Papp, Balázs, Oszi, Erika, Magyar, Zoltán, Marsh, Joseph A., Elcock, Adrian H., Babu, M. Madan, Robinson, Carol V., Sugimoto, Naoki, Teichmann, Sarah A.

“…Cotranslational protein folding can facilitate rapid formation of functional structures. However, it can also cause premature assembly of protein complexes, if…”
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Mass spectrometry guided structural biology by Liko, Idlir, Allison, Timothy M, Hopper, Jonathan TS, Robinson, Carol V

“…•Mass spectrometry provides unambiguous determination of subunit stoichiometry.•Defines the binding of lipids or other small molecules.•Describes…”
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Metabolite collision cross section prediction without energy-minimized structures by Soper-Hopper, M T, Vandegrift, J, Baker, E S, Fernández, F M

“…Matching experimental ion mobility-mass spectrometry data to computationally-generated collision cross section (CCS) values enables more confident metabolite…”
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Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces by Ambrose, Stephen, Housden, Nicholas G., Gupta, Kallol, Fan, Jieyuan, White, Paul, Yen, Hsin‐Yung, Marcoux, Julien, Kleanthous, Colin, Hopper, Jonathan T. S., Robinson, Carol V.

“…Mass spectrometry (MS) applications for intact protein complexes typically require electrospray (ES) ionization and have not been achieved via direct…”
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A combined computational and structural model of the full-length human prolactin receptor by Bugge, Katrine, Papaleo, Elena, Haxholm, Gitte W., Hopper, Jonathan T. S., Robinson, Carol V., Olsen, Johan G., Lindorff-Larsen, Kresten, Kragelund, Birthe B.

“…The prolactin receptor is an archetype member of the class I cytokine receptor family, comprising receptors with fundamental functions in biology as well as…”
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Colicin-Mediated Transport of DNA through the Iron Transporter FepA by Cohen-Khait, Ruth, Harmalkar, Ameya, Pham, Phuong, Webby, Melissa N, Housden, Nicholas G, Elliston, Emma, Hopper, Jonathan T S, Mohammed, Shabaz, Robinson, Carol V, Gray, Jeffrey J, Kleanthous, Colin

“…Colicins are protein antibiotics deployed by Escherichia coli to eliminate competing strains. Colicins frequently exploit outer membrane (OM) nutrient…”
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The human Na(+)/H(+) exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2 by Hendus-Altenburger, Ruth, Pedraz-Cuesta, Elena, Olesen, Christina W, Papaleo, Elena, Schnell, Jeff A, Hopper, Jonathan T S, Robinson, Carol V, Pedersen, Stine F, Kragelund, Birthe B

“…Extracellular signal-regulated kinase 2 (ERK2) is an S/T kinase with more than 200 known substrates, and with critical roles in regulation of cell growth and…”
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A diverse proteome is present and enzymatically active in metabolite extracts by House, Rachel (Rae) J., Soper-Hopper, Molly T., Vincent, Michael P., Ellis, Abigail E., Capan, Colt D., Madaj, Zachary B., Wolfrum, Emily, Isaguirre, Christine N., Castello, Carlos D., Johnson, Amy B., Escobar Galvis, Martha L., Williams, Kelsey S., Lee, Hyoungjoo, Sheldon, Ryan D.

“…Metabolite extraction is the critical first-step in metabolomics experiments, where it is generally regarded to inactivate and remove proteins. Here, arising…”
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Mass Spectrometry Quantifies Protein Interactions-From Molecular Chaperones to Membrane Porins by Hopper, Jonathan T. S., Robinson, Carol V.

“…Proteins possess an intimate relationship between their structure and function, with folded protein structures generating recognition motifs for the binding of…”
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Elongation of Model Prebiotic Proto-Peptides by Continuous Monomer Feeding by Yu, Sheng-Sheng, Solano, Martin D, Blanchard, Matthew K, Soper-Hopper, Molly T, Krishnamurthy, Ramanarayanan, Fernández, Facundo M, Hud, Nicholas V, Schork, F. Joseph, Grover, Martha A

“…Mixtures of amino acids with hydroxy acids allow for the formation of peptide bonds in a plausible prebiotic scenario via ester bond formation followed by…”
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Effects of CYP46A1 Inhibition on Long-Term-Depression in Hippocampal Slices ex vivo and 24S-Hydroxycholesterol Levels in Mice in vivo by Popiolek, Michael, Izumi, Yukitoshi, Hopper, Allen T., Dai, Jing, Miller, Silke, Shu, Hong-Jin, Zorumski, Charles F., Mennerick, Steven

“…The manipulation of cholesterol and its metabolites has been hypothesized to be therapeutically beneficial for mood disorders, neurodegenerative disorders, and…”
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Intrinsically Disordered Protein Threads Through the Bacterial Outer-Membrane Porin OmpF by Housden, Nicholas G., Hopper, Jonathan T. S., Lukoyanova, Natalya, Rodriguez-Larrea, David, Wojdyla, Justyna A., Klein, Alexander, Kaminska, Renata, Bayley, Hagan, Saibil, Helen R., Robinson, Carol V., Kleanthous, Colin

“…Porins are β-barrel outer-membrane proteins through which small solutes and metabolites diffuse that are also exploited during cell death. We have studied how…”
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Identifying key membrane protein lipid interactions using mass spectrometry by Gupta, Kallol, Li, Jingwen, Liko, Idlir, Gault, Joseph, Bechara, Cherine, Wu, Di, Hopper, Jonathan T S, Giles, Kevin, Benesch, Justin L P, Robinson, Carol V

“…This protocol describes a native mass-spectrometry-based approach for identifying the key lipids that interact with membrane proteins. With the recent success…”
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The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core by Rajasekar, Karthik V., Zdanowski, Konrad, Yan, Jun, Hopper, Jonathan T. S., Francis, Marie-Louise R., Seepersad, Colin, Sharp, Connor, Pecqueur, Ludovic, Werner, Jörn M., Robinson, Carol V., Mohammed, Shabaz, Potts, Jennifer R., Kleanthous, Colin

“…Redox-regulated effector systems that counteract oxidative stress are essential for all forms of life. Here we uncover a new paradigm for sensing oxidative…”
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