Enzyme Modification of Platinum Microelectrodes for Detection of Cholesterol in Vesicle Lipid Bilayer Membranes

Enzyme Modification of Platinum Microelectrodes for Detection of Cholesterol in Vesicle Lipid Bilayer Membranes

Platinum microelectrodes are modified with a lipid bilayer membrane incorporating cholesterol oxidase. Details for electrode surface modification are presented along with characterization studies of electrode response to cholesterol solution and to cholesterol contained in the lipid bilayer membrane...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 77; no. 22; pp. 7393 - 7398
Main Authors: Devadoss, Anando, Palencsár, M. Simona, Jiang, Dechen, Honkonen, Michael L, Burgess, James D
Format: Journal Article
Language:English
Published: Washington, DC American Chemical Society 15-11-2005
Subjects:
Analytical chemistry
Chemistry
Cholesterol
Cholesterol - analysis
Cholesterol - metabolism
Electrochemical methods
Electrodes
Enzymes
Exact sciences and technology
Glycine max
Lipid Bilayers
Lipids
Membranes
Microelectrodes
Oxidoreductases - metabolism
Platinum - chemistry
Thiol
Enzyme
Lipids
Voltammetry
Bilayer
Steady state
Cholesterol
Cyclodextrin
Chemical modification
Platinum
Microelectrode
Membrane
Hexacyanoferrates II
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Summary:Platinum microelectrodes are modified with a lipid bilayer membrane incorporating cholesterol oxidase. Details for electrode surface modification are presented along with characterization studies of electrode response to cholesterol solution and to cholesterol contained in the lipid bilayer membrane of vesicles. Ferrocyanide voltammetric experiments are used to track deposition of a submonolayer of a thiol-functionalized lipid on the platinum electrode surface, vesicle fusion for bilayer formation on the thiolipid-modified surface, and incorporation of cholesterol oxidase in the electrode-supported thiolipid/lipid bilayer membrane. The data are consistent with formation of a lipid bilayer structure on the electrode surface that contains defects. Experiments for detection of cholesterol solubilized in cyclodextrin solution show steady-state current responses that correlate with cholesterol concentration. Direct contact between the electrode and a vesicle lipid bilayer membrane shows a response that correlates with vesicle membrane cholesterol content.
Bibliography:istex:E0E221D90BFDDDB31B53D087AD1C0131085B74C3
ark:/67375/TPS-4G64LS0F-M
ObjectType-Article-1
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ISSN:0003-2700
1520-6882
DOI:10.1021/ac051173f