Inhibition of IL-13 and IL-13Rα2 Expression by IL-32θ in Human Monocytic Cells Requires PKCδ and STAT3 Association

Inhibition of IL-13 and IL-13Rα2 Expression by IL-32θ in Human Monocytic Cells Requires PKCδ and STAT3 Association

Interleukin (IL)-32θ, a newly identified IL-32 isoform, has been reported to exert pro-inflammatory effects through the association with protein kinase C delta (PKCδ). In this study, we further examined the effects of IL-32θ on IL-13 and IL-13Rα2 expression and the related mechanism in THP-1 cells....

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 20; no. 8; p. 1949
Main Authors: Pham, Thu-Huyen, Bak, Yesol, Oh, Jae-Wook, Hong, Jingi, Lee, Seungyeoun, Hong, Jin Tae, Yoon, Do-Young
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 20-04-2019
MDPI
Subjects:
Binding sites
Cell surface
Deoxyribonucleic acid
Dimerization
DNA
Flow cytometry
Genes
IL-13 signaling
IL-13Rα2
IL-32θ
Inflammation
Interleukin 13
Kinases
Monocytes
Nuclear transport
Phosphorylation
PKCδ
Protein expression
Protein kinase C
Proteins
Regulatory sequences
Serine
Signal transduction
STAT3 activation
Stat3 protein
Stat6 protein
Transcription factors
Tyrosine
PKCδ
IL-32θ
IL-13Rα2
STAT3 activation
IL-13 signaling
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Summary:Interleukin (IL)-32θ, a newly identified IL-32 isoform, has been reported to exert pro-inflammatory effects through the association with protein kinase C delta (PKCδ). In this study, we further examined the effects of IL-32θ on IL-13 and IL-13Rα2 expression and the related mechanism in THP-1 cells. Upon stimulating IL-32θ-expressing and non-expressing cells with phorbol 12-myristate 13-acetate (PMA), the previous microarray analysis showed that IL-13Rα2 and IL-13 mRNA expression were significantly decreased by IL-32θ. The protein expression of these factors was also confirmed to be down-regulated. The nuclear translocation of transcription factors STAT3 and STAT6, which are necessary for IL-13Rα2 and IL-13 promoter activities, was suppressed by IL-32θ. Additionally, a direct association was found between IL-32θ, PKCδ, and signal transducer and activator of transcription 3 (STAT3), but not STAT6, revealing that IL-32θ might act mainly through STAT3 and indirectly affect STAT6. Moreover, the interaction of IL-32θ with STAT3 requires PKCδ, since blocking PKCδ activity eliminated the interaction and consequently limited the inhibitory effect of IL-32θ on STAT3 activity. Interfering with STAT3 or STAT6 binding by decoy oligodeoxynucleotides (ODNs) identified that IL-32θ had additive effects with the STAT3 decoy ODN to suppress IL-13 and IL-13Rα2 mRNA expression. Taken together, our data demonstrate the intracellular interaction of IL-32θ, PKCδ, and STAT3 to regulate IL-13 and IL-13Rα2 synthesis, supporting the role of IL-32θ as an inflammatory modulator.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20081949