Novel portable electrical detection system for DNA SENSOR application

Novel portable electrical detection system for DNA SENSOR application

A novel, portable, electrical detection system was constructed for DNA sensor application to detect DNA hybridisation. The read-out circuit consists of an analogue circuit and a digital circuit. The analogue circuit with an IC MAX038 generates a sine wave with a constant frequency (10 kHz), which se...

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Bibliographic Details
Published in:Journal of experimental nanoscience Vol. 9; no. 7; pp. 652 - 660
Main Authors: Tam, Phuong Dinh, Hong, Hoang Sy, Hieu, Nguyen Van
Format: Journal Article
Language:English
Published: Taylor & Francis Group 01-01-2014
Subjects:
Amplitudes
Analogue
Circuits
Deoxyribonucleic acid
Digital circuits
DNA sensor
label-free
Portability
readout system
Sensors
Sine waves
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Summary:A novel, portable, electrical detection system was constructed for DNA sensor application to detect DNA hybridisation. The read-out circuit consists of an analogue circuit and a digital circuit. The analogue circuit with an IC MAX038 generates a sine wave with a constant frequency (10 kHz), which serves as the input for the DNA sensor. The DNA sensor consists of active and reference sensors. DNA hybridisation between the DNA probe and the target sequences causes changes in the conductance of the conductive membrane (DNA/MWCNTs) on the sensor surface, which lead to changes in the amplitude of the sine wave from the sensor output compared with that of the reference signal output (input sine wave). We used a digital circuit with a microprocessor (PIC33FJ256MC710) to determine the change in the amplitude of the sine wave signal of the sensor. From these digital data, the difference in the amplitudes of the active and reference sensors was calculated and displayed on the liquid crystal display. Measurement results show that the portable electrical detection system can detect DNA target concentrations as low as 0.16 µM. The detection of the amplified polymerase chain reaction sample and the reproducibility of the DNA sensor results were also determined using the designed readout circuit. The proposed electrical detection system is suitable for DNA sensor application.
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ISSN:1745-8080
1745-8099
DOI:10.1080/17458080.2012.683536