Most Cases of Cryptococcal Meningitis in HIV-Uninfected Patients in Vietnam Are Due to a Distinct Amplified Fragment Length Polymorphism-Defined Cluster of Cryptococcus neoformans var. grubii VN1

Cryptococcal disease most commonly occurs in patients with an underlying immune deficit, most commonly HIV infection, and is due to Cryptococcus neoformans var. grubii. Occasionally disease due to this variety occurs in apparently immunocompetent patients. The relationship between strains infecting...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of Clinical Microbiology Vol. 49; no. 2; pp. 658 - 664
Main Authors:	Day, Jeremy N, Hoang, Thu N, Duong, Anh V, Hong, Chau T.T, Diep, Pham T, Campbell, James I, Sieu, Tran P.M, Hien, Tran T, Bui, Tien, Boni, Maciej F, Lalloo, David G, Carter, Dee, Baker, Stephen, Farrar, Jeremy J
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-02-2011
Subjects:	Adolescent Adult Aged Amplified Fragment Length Polymorphism Analysis Biological and medical sciences Cluster Analysis Cryptococcus neoformans Cryptococcus neoformans - classification Cryptococcus neoformans - genetics Cryptococcus neoformans - isolation & purification DNA, Fungal - genetics Female Fundamental and applied biological sciences. Psychology Genotype HIV Infections - complications Human immunodeficiency virus Human viral diseases Humans Infectious diseases Male Medical sciences Meningitis, Cryptococcal - microbiology Microbiology Middle Aged Miscellaneous Molecular Epidemiology Mycological Typing Techniques Mycology Vietnam Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Young Adult South east Asia Vietnam Asia Provence Alpes Côte d'Azur Europe Var France Immunopathology Nervous system diseases Yeast Cryptococcus neoformans Tropical zone AIDS Immune deficiency Fungi Infection Viral disease Central nervous system disease Meningitis Fungi Imperfecti Mediterranean region Amplified fragment length polymorphism marker
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Cryptococcal disease most commonly occurs in patients with an underlying immune deficit, most commonly HIV infection, and is due to Cryptococcus neoformans var. grubii. Occasionally disease due to this variety occurs in apparently immunocompetent patients. The relationship between strains infecting immunosuppressed and immunocompetent patients is not clear. Amplified fragment length polymorphism (AFLP) analysis was used to characterize the relationship between strains infecting HIV-infected and uninfected patients. Isolates from 51 HIV-uninfected patients and 100 HIV-infected patients with cryptococcal meningitis were compared. C. neoformans var. grubii VNI was responsible for infections in 73% of HIV-uninfected and 100% of HIV-infected patients. AFLP analysis defined two distinct clusters, VNIγ and VNIδ. The majority (84%) of isolates from HIV-uninfected patients were VNIγ, compared with only 38% of isolates from HIV-infected patients (odds ratio, 8.30; 95% confidence interval [CI], 3.04 to 26.6; P < 0.0001). In HIV-uninfected patients, underlying disease was less frequent in those with VNIγ infections. Two clusters of C. neoformans var. grubii VN1 are responsible for the majority of cases of cryptococcal meningitis in Vietnam. The distribution of these clusters differs according to the immune status of the host.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.01985-10