Search Results - "Honegger, Annemarie"

Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action by Kast, Florian, Schwill, Martin, Stüber, Jakob C., Pfundstein, Svende, Nagy-Davidescu, Gabriela, Rodríguez, Josep M. Monné, Seehusen, Frauke, Richter, Christian P., Honegger, Annemarie, Hartmann, Karen Patricia, Weber, Thomas G., Kroener, Felix, Ernst, Patrick, Piehler, Jacob, Plückthun, Andreas

“…The receptor tyrosine kinase HER2 acts as oncogenic driver in numerous cancers. Usually, the gene is amplified, resulting in receptor overexpression, massively…”
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Structural Basis for Eliciting a Cytotoxic Effect in HER2-Overexpressing Cancer Cells via Binding to the Extracellular Domain of HER2 by Jost, Christian, Schilling, Johannes, Tamaskovic, Rastislav, Schwill, Martin, Honegger, Annemarie, Plückthun, Andreas

“…Human epidermal growth factor receptor-2 (HER2) is a receptor tyrosine kinase directly linked to the growth of malignancies from various origins and a…”
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Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control by Schmid, Markus, Ernst, Patrick, Honegger, Annemarie, Suomalainen, Maarit, Zimmermann, Martina, Braun, Lukas, Stauffer, Sarah, Thom, Cristian, Dreier, Birgit, Eibauer, Matthias, Kipar, Anja, Vogel, Viola, Greber, Urs F., Medalia, Ohad, Plückthun, Andreas

“…Most systemic viral gene therapies have been limited by sequestration and degradation of virions, innate and adaptive immunity, and silencing of therapeutic…”
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DARPins Recognizing the Tumor-Associated Antigen EpCAM Selected by Phage and Ribosome Display and Engineered for Multivalency by Stefan, Nikolas, Martin-Killias, Patricia, Wyss-Stoeckle, Sascha, Honegger, Annemarie, Zangemeister-Wittke, Uwe, Plückthun, Andreas

“…Designed Ankyrin Repeat Proteins (DARPins) represent a novel class of binding molecules. Their favorable biophysical properties such as high affinity,…”
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Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations by Schlinkmann, Karola M, Honegger, Annemarie, Türeci, Esin, Robison, Keith E, Lipovšek, Daša, Plückthun, Andreas

“…The structural features determining efficient biosynthesis, stability in the membrane and, after solubilization, in detergents are not well understood for…”
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Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS–p110 crosstalk by Tamaskovic, Rastislav, Schwill, Martin, Nagy-Davidescu, Gabriela, Jost, Christian, Schaefer, Dagmar C., Verdurmen, Wouter P. R., Schaefer, Jonas V., Honegger, Annemarie, Plückthun, Andreas

“…Compensatory mechanisms, such as relief of AKT-ErbB3-negative feedback, are known to desensitize ErbB2-dependent tumours to targeted therapy. Here we describe…”
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Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters by Dreier, Birgit, Honegger, Annemarie, Hess, Christian, Nagy-Davidescu, Gabriela, Mittl, Peer R E, Grütter, Markus G, Belousova, Natalya, Mikheeva, Galina, Krasnykh, Victor, Plückthun, Andreas

“…Adenoviruses (Ads) have shown promise as vectors for gene delivery in clinical trials. Efficient viral targeting to a tissue of choice requires both ablation…”
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Yet Another Numbering Scheme for Immunoglobulin Variable Domains: An Automatic Modeling and Analysis Tool by Honegger, Annemarie, Plückthun, Andreas

“…A common residue numbering scheme for all immunoglobulin variable domains (immunoglobulin light chain lambda (V λ) and kappa (V κ) variable domains, heavy…”
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Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition by Reichen, Christian, Hansen, Simon, Forzani, Cristina, Honegger, Annemarie, Fleishman, Sarel J., Zhou, Ting, Parmeggiani, Fabio, Ernst, Patrick, Madhurantakam, Chaithanya, Ewald, Christina, Mittl, Peer R.E., Zerbe, Oliver, Baker, David, Caflisch, Amedeo, Plückthun, Andreas

“…Armadillo repeat proteins (ArmRPs) recognize their target peptide in extended conformation and bind, in a first approximation, two residues per repeat. Thus,…”
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The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains by Honegger, Annemarie, Malebranche, Alain Daniel, Röthlisberger, Daniela, Plückthun, Andreas

“…Antibody variable domains differ considerably in stability. Single-chain Fv (scFv) fragments derived from natural repertoires frequently lack the high…”
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Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv by Friedel, Thorsten, Hanisch, Lydia J., Muth, Anke, Honegger, Annemarie, Abken, Hinrich, Plückthun, Andreas, Buchholz, Christian J., Schneider, Irene C.

“…An increasing number of applications require the expression of single-chain variable fragments (scFv) fusion proteins in mammalian cells at the cell surface…”
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Dual-targeting triplebody 33-16-123 (SPM-2) mediates effective redirected lysis of primary blasts from patients with a broad range of AML subtypes in combination with natural killer cells by Braciak, Todd A., Roskopf, Claudia C., Wildenhain, Sarah, Fenn, Nadja C., Schiller, Christian B., Schubert, Ingo A., Jacob, Uwe, Honegger, Annemarie, Krupka, Christina, Subklewe, Marion, Spiekermann, Karsten, Hopfner, Karl-Peter, Fey, Georg H., Aigner, Michael, Krause, Stefan, Mackensen, Andreas, Oduncu, Fuat S.

“…A number of agents designed for immunotherapy of Acute Myeloid Leukemia (AML) are in preclinical and early clinical development. Most of them target a single…”
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A Combined NMR and Computational Approach to Investigate Peptide Binding to a Designed Armadillo Repeat Protein by Ewald, Christina, Christen, Martin T., Watson, Randall P., Mihajlovic, Maja, Zhou, Ting, Honegger, Annemarie, Plückthun, Andreas, Caflisch, Amedeo, Zerbe, Oliver

“…The specific recognition of peptide sequences by proteins plays an important role both in biology and in diagnostic applications. Here we characterize the…”
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Tailoring in vitro Evolution for Protein Affinity or Stability by Jermutus, Lutz, Honegger, Annemarie, Schwesinger, Falk, Hanes, Jozef, Plückthun, Andreas

“…We describe a rapid and general technology working entirely in vitro to evolve either the affinity or the stability of ligand-binding proteins, depending on…”
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Unbinding Forces of Single Antibody-Antigen Complexes Correlate with Their Thermal Dissociation Rates by Schwesinger, F, Ros, R, Strunz, T, Anselmetti, D, Güntherodt, H J, Honegger, A, Jermutus, L, Tiefenauer, L, Pluckthun, A

“…Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine different single-chain Fv fragments, whose on-rates, off-rates, and…”
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Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system by Krebber, Anke, Bornhauser, Susanne, Burmester, Jörg, Honegger, Annemarie, Willuda, Jörg, Bosshard, Hans Rudolf, Plückthun, Andreas

“…A prerequisite for the use of recombinant antibody technologies starting from hybridomas or immune repertoires is the reliable cloning of functional…”
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The Influence of the Buried Glutamine or Glutamate Residue in Position 6 on the Structure of Immunoglobulin Variable Domains by Honegger, Annemarie, Plückthun, Andreas

“…Immunoglobulin V H domain frameworks can be grouped into four distinct types, depending on the main-chain conformation of framework 1. Based on the analysis of…”
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Direct in Vivo Screening of Intrabody Libraries Constructed on a Highly Stable Single-chain Framework by der Maur, Adrian Auf, Zahnd, Christian, Fischer, Franziska, Spinelli, Silvia, Honegger, Annemarie, Cambillau, Christian, Escher, Dominik, Plückthun, Andreas, Barberis, Alcide

“…Single-chain Fv antibody fragments (scFv) represent a convenient antibody format for intracellular expression in eukaryotic or prokaryotic cells. These…”
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Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library by Blackburn, G Michael, Plückthun, Andreas, Cesaro-Tadic, Sandro, Lagos, Dimitrios, Honegger, Annemarie, Rickard, James H, Partridge, Lynda J

“…This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro…”
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Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors by Wörn, Arne, Auf der Maur, Adrian, Escher, Dominik, Honegger, Annemarie, Barberis, Alcide, Plückthun, Andreas

“…A cellular assay system for measuring the activity of cytoplasmically expressed anti-GCN4 scFv fragments directed against the Gcn4p dimerization domain was…”
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