Online monitoring of cell metabolism for studying pharmacodynamic effects

To characterize modes of action of substances and their cytotoxic effects Bionas GmbH has developed a new screening system to allow the continuous recording of how an active substance can act (Bionas® 2500 analyzing system). In the pharmaceutical industry it is important to acquire as much informati...

Full description

Saved in:

Bibliographic Details
Published in:	Toxicology and applied pharmacology Vol. 220; no. 1; pp. 33 - 44
Main Authors:	Thedinga, Elke, Kob, Axel, Holst, Heiko, Keuer, Andreas, Drechsler, Sabine, Niendorf, Ricarda, Baumann, Werner, Freund, Ingo, Lehmann, Mirko, Ehret, Ralf
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-04-2007 Elsevier
Subjects:	60 APPLIED LIFE SCIENCES ACIDIFICATION Adenosine Triphosphate - metabolism Adhesion Animals Biological and medical sciences Biosensing Techniques - instrumentation Cell Adhesion Cell impedance Cell Line Cell metabolism Confluence Cycloheximide - pharmacology DRUGS ELECTRODES Extracellular acidification FIELD EFFECT TRANSISTORS Humans Hydrogen-Ion Concentration IMPEDANCE IN VITRO Label-free Medical sciences METABOLISM MONITORING Monitoring, Physiologic - instrumentation Non-invasive Online monitoring OXYGEN Oxygen Consumption Pharmacodynamic Recovery REGENERATION Toxicology Non-invasive Cell metabolism Pharmacodynamic Oxygen consumption Online monitoring In vitro Adhesion Recovery Cell impedance Regeneration Toxicology Confluence Extracellular acidification Label-free Pharmacodynamics Acidification Metabolism Extracellular Surveillance Impedance
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	To characterize modes of action of substances and their cytotoxic effects Bionas GmbH has developed a new screening system to allow the continuous recording of how an active substance can act (Bionas® 2500 analyzing system). In the pharmaceutical industry it is important to acquire as much information as possible about the metabolic effects of an active substance. Most classical pre-clinical studies are very expensive and time-consuming. Often they are so-called end-point tests which require many individual tests before approximate statements can be made about how an effect takes its course. With the Bionas® 2500 analyzing system metabolically relevant data including oxygen consumption, acidification rate and the adhesion (cell impedance) of cells can be measured in parallel, online and label-free. Using e.g. ion-sensitive field effect-transistors (ISFET) and electrode structures it is possible to observe metabolic parameters non-invasively and continuously over longer periods of time. The system has already been established for several cell models, cell lines as well as primary cells. It also offers the advantage that regenerative effects can be observed during the same test run.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-008X 1096-0333
DOI:	10.1016/j.taap.2006.12.027