Search Results - "Holowka, D"

Crosslinking a Lipid Raft Component Triggers Liquid Ordered-Liquid Disordered Phase Separation in Model Plasma Membranes by Hammond, A. T., Heberle, F. A., Baumgart, T., Holowka, D., Baird, B., Feigenson, G. W., Simons, Kai

“…The mechanisms by which a cell uses and adapts its functional membrane organization are poorly understood and are the subject of ongoing investigation and…”
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Fluorescence Anisotropy Measurements of Lipid Order in Plasma Membranes and Lipid Rafts from RBL-2H3 Mast Cells by Gidwani, Arun, Holowka, David, Baird, Barbara

“…Specialized plasma membrane domains known as lipid rafts participate in signal transduction and other cellular processes, and their liquid ordered (Lo) phase…”
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In vitro modelling of rat mucosal mast cell function in Trichinella spiralis infection by Thrasher, S. M., Scalfone, L. K., Holowka, D., Appleton, J. A.

“…Summary Intestinal infection with the parasitic nematode, Trichinella spiralis, provides a robust context for the study of mucosal mast cell function. In rats,…”
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Mutant RBL mast cells defective in Fc epsilon RI signaling and lipid raft biosynthesis are reconstituted by activated Rho-family GTPases by Field, K A, Apgar, J R, Hong-Geller, E, Siraganian, R P, Baird, B, Holowka, D

“…Characterization of defects in a variant subline of RBL mast cells has revealed a biochemical event proximal to IgE receptor (Fc epsilon RI)-stimulated…”
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Lipid rafts, fluid/fluid phase separation, and their relevance to plasma membrane structure and function by Sengupta, Prabuddha, Baird, Barbara, Holowka, David

“…Novel biophysical approaches combined with modeling and new biochemical data have helped to recharge the lipid raft field and have contributed to the…”
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Molecular Crowding on the Cell Surface by Ryan, T. A., Myers, J., Holowka, D., Baird, B., Webb, W. W.

“…Strong steric interactions among proteins on crowded living cell surfaces were revealed by measurements of the equilibrium spatial distributions of proteins in…”
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The Fc Segment of IgE Influences the Kinetics of Dissociation of a Symmetrical Bivalent Ligand from Cyclic Dimeric Complexes by Subramanian, Kala, Holowka, David, Baird, Barbara, Goldstein, Byron

“…As part of a systematic effort to determine the features of immunoglobulin E−receptor (IgE−FcεRI) aggregation that are critical for cellular activation, we…”
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Cholera toxin increases the rate of antigen-stimulated calcium influx in rat basophilic leukemia cells by Narasimhan, V, Holowka, D, Fewtrell, C, Baird, B

“…Cholera toxin pretreatment has been found to cause a 3-fold increase in the initial rate of antigen-stimulated secretion of serotonin from rat basophilic…”
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Heterologous desensitization of the high affinity receptor for IgE (Fc epsilon R1) on RBL cells by Weetall, M, Holowka, D, Baird, B

“…To study the properties of Fc epsilon RI desensitization induced by aggregation of that receptor, RBL cells were sensitized with a mixture of two different IgE…”
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Bivalent binding of an anti-CD3 antibody to Jurkat cells induces association of the T cell receptor complex with the cytoskeleton by Marano, N, Holowka, D, Baird, B

“…Ligand binding and cross-linking of TCR/CD3 complex leads to T cell stimulation in immunologic responses. As a prelude to investigating the dynamic…”
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The effect of receptor density on the forward rate constant for binding of ligands to cell surface receptors by Erickson, J., Goldstein, B., Holowka, D., Baird, B.

“…For monovalent ligands interacting with cell surface receptors we have directly observed the functional dependence of the forward rate constant on the number…”
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Critical Role for Cholesterol in Lyn-Mediated Tyrosine Phosphorylation of FcεRI and Their Association with Detergent-Resistant Membranes by Sheets, Erin D., Holowka, David, Baird, Barbara

“…Tyrosine phosphorylation of the high affinity immunoglobulin (Ig)E receptor (FcεRI) by the Src family kinase Lyn is the first known biochemical step that…”
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A guanine nucleotide-binding protein participates in IgE receptor-mediated activation of endogenous and reconstituted phospholipase A2 in a permeabilized cell system by Narasimhan, V, Holowka, D, Baird, B

“…Activation of phospholipase A2 (PLA2) by the aggregation of receptors for immunoglobulin E (IgE) can be studied in streptolysin O-permeabilized rat basophilic…”
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Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells by Robertson, D, Holowka, D, Baird, B

“…Bridging of immunoglobulin E (IgE)-receptor complexes on rat basophilic leukemia cells by polyclonal anti-IgE antibodies induces a detergent-resistant…”
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Fc epsilon RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling by Field, K A, Holowka, D, Baird, B

“…Detergent-resistant plasma membrane structures, such as caveolae, have been implicated in signalling, transport, and vesicle trafficking functions. Using…”
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Interactions between FcεRI and lipid raft components are regulated by the actin cytoskeleton by Holowka, David, Sheets, Erin D., Baird, Barbara

“…ABSTRACT Previous studies showed that crosslinking of IgE-FcεRI complexes on RBL-2H3 mast cells causes their association with isolated detergent-resistant…”
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A Lipid Raft Environment Enhances Lyn Kinase Activity by Protecting the Active Site Tyrosine from Dephosphorylation by Young, Ryan M., Holowka, David, Baird, Barbara

“…The plasma membrane contains ordered lipid domains, commonly called lipid rafts, enriched in cholesterol, sphingolipids, and certain signaling proteins. Lipid…”
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Characterization of increased K+ permeability associated with the stimulation of receptors for immunoglobulin E on rat basophilic leukemia cells by LABRECQUE, G. F, HOLOWKA, D, BAIRD, B

“…Aggregation of immunoglobulin E-receptor complexes on the surface of rat basophilic leukemia cells stimulates an increase in plasma membrane K+ permeability…”
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Visualization of Plasma Membrane Compartmentalization with Patterned Lipid Bilayers by Wu, Min, Holowka, David, Craighead, Harold G., Baird, Barbara, Simons, Kai

“…Micrometer-size patterned lipid bilayers containing liganded lipids are used to control the location and size of receptor clusters and enable direct…”
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Competition between solution and cell surface receptors for ligand. Dissociation of hapten bound to surface antibody in the presence of solution antibody by Goldstein, B., Posner, R.G., Torney, D.C., Erickson, J., Holowka, D., Baird, B.

“…We present a joint theoretical and experimental study on the effects of competition for ligand between receptors in solution and receptors on cell surfaces. We…”
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