Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis

The Drosophila slit gene ( sli) encodes a secreted leucine-rich repeat-containing protein (slit) expressed by the midline glial cells and required for normal neural development. A putative human sli homolog, SLIT1, has previously been identified by EST database scanning. We have isolated a second hu...

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Published in:	Mechanisms of development Vol. 79; no. 1; pp. 57 - 72
Main Authors:	Holmes, Greg P., Negus, Kylie, Burridge, Linda, Raman, Sunil, Algar, Elizabeth, Yamada, Toshiya, Little, Melissa H.
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 01-12-1998
Subjects:	Agrin–laminin–perlecan–slit domain Amino Acid Sequence Animals Brain Brain - embryology Cell Line Central Nervous System - embryology Cloning, Molecular CNS development Drosophila Proteins EGF repeat Embryo, Mammalian - metabolism Embryo, Nonmammalian Expressed Sequence Tags Extremities - embryology Female Floor plate Gene Expression Regulation, Developmental Humans In Situ Hybridization Infant, Newborn Intercellular Signaling Peptides and Proteins Leucine-rich repeat Limb development Male Metanephric mesenchyme Mice Midline glial cells Molecular Sequence Data Motor neurons Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Notochord Organizer Protein–protein interactions Roof plate Sequence Analysis Sequence Homology, Amino Acid Spinal cord Spinal Cord - embryology Spinal Cord - growth & development Urogenital System - growth & development Vertebrates Motor neurons Brain Roof plate Spinal cord Protein–protein interactions Organizer Leucine-rich repeat CNS development Agrin–laminin–perlecan–slit domain Midline glial cells Floor plate Metanephric mesenchyme EGF repeat Notochord Limb development
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Summary:	The Drosophila slit gene ( sli) encodes a secreted leucine-rich repeat-containing protein (slit) expressed by the midline glial cells and required for normal neural development. A putative human sli homolog, SLIT1, has previously been identified by EST database scanning. We have isolated a second human sli homolog, SLIT2, and its murine homolog Slit2. Both SLIT1 and SLIT2 proteins show approximately 40% amino acid identity to slit and 60% identity to each other. In mice, both genes are expressed during CNS development in the floor plate, roof plate and developing motor neurons. As floor plate represents the vertebrate equivalent to the midline glial cells, we predict a conservation of function for these vertebrate homologs. Each gene shows additional but distinct sites of expression outside the CNS suggesting a variety of functions for these proteins.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0925-4773 1872-6356
DOI:	10.1016/S0925-4773(98)00174-9