Early life lead exposure causes gender-specific changes in the DNA methylation profile of DNA extracted from dried blood spots

Early life lead exposure causes gender-specific changes in the DNA methylation profile of DNA extracted from dried blood spots

In this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on the sex of the child and can serve as biomarkers for Pb exposure. In this pilot study, we measured the 5mC profiles of DNA extracted from dried blood spots (DBS) in a...

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Bibliographic Details
Published in:Epigenomics Vol. 7; no. 3; pp. 379 - 393
Main Authors: Sen, Arko, Heredia, Nicole, Senut, Marie-Claude, Hess, Matthew, Land, Susan, Qu, Wen, Hollacher, Kurt, Dereski, Mary O, Ruden, Douglas M
Format: Journal Article
Language:English
Published: England Future Medicine Ltd 01-06-2015
Subjects:
Analysis
Antioxidants (Nutrients)
Biomarkers
Blood
blood lead levels
Child, Preschool
Children & youth
Deoxyribonucleic acid
differential methylation
DNA
DNA - blood
DNA - isolation & purification
DNA methylation
DNA Methylation - drug effects
dried blood spots
Environmental Exposure
Epigenetics
Exposure
Families & family life
Female
Females
Food contamination & poisoning
Gender
gene specific
Genes
Genes, Mitochondrial
Genetic polymorphisms
Genomes
Genomics
Health aspects
Humans
Infant
Lead
Lead - blood
Lead - pharmacology
Lead content
Loci
Male
Males
Methylation
Physiology
Pilot Projects
Sex
Sex Factors
Visual cortex
whole blood
United States
United States > US
California
gene specific
DNA methylation
whole blood
blood lead levels
differential methylation
dried blood spots
epigenetics
lead
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Summary:In this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on the sex of the child and can serve as biomarkers for Pb exposure. In this pilot study, we measured the 5mC profiles of DNA extracted from dried blood spots (DBS) in a cohort of 43 children (25 males and 18 females; ages from 3 months to 5 years) from Detroit. We found that the effect of Pb-exposure on the 5-mC profiles can be separated into three subtypes: affected methylation loci which are conserved irrespective of the sex of the child (conserved); affected methylation loci unique to males (male-specific); and affected methylation loci unique to females (female-specific).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1750-1911
1750-192X
DOI:10.2217/epi.15.2