Search Results - "Holden, M.J"

Structure of Alanine Dehydrogenase from Archaeoglobus: Active Site Analysis and Relation to Bacterial Cyclodeaminases and Mammalian mu Crystallin by Gallagher, D.T., Monbouquette, H.G., Schröder, I., Robinson, H., Holden, M.J., Smith, N.N.

“…The hyperthermophilic archaeon Archaeoglobus fulgidus contains an l-Ala dehydrogenase (AlaDH, EC 1.4.1.1) that is not homologous to known bacterial…”
Get full text

A thermodynamic investigation of some reactions involving prephenic acid by Kishore, N, Holden, M.J, Tewari, Y.B, Goldberg, R.N

“…Calorimetric enthalpies of reaction have been measured for the following enzyme-catalysed reactions at the temperature 298.15 K: prephenate(aq) =…”
Get full text

Toward Metrological Traceability for DNA Fragment Ratios in GM Quantification. 1. Effect of DNA Extraction Methods on the Quantitative Determination of Bt176 Corn by Real-Time PCR by Corbisier, Philippe, Broothaerts, Wim, Gioria, Sabrina, Schimmel, Heinz, Burns, Malcolm, Baoutina, Anna, Emslie, Kerry R, Furui, Satoshi, Kurosawa, Yasunori, Holden, Marcia J, Kim, Hyong-Ha, Lee, Yun-mi, Kawaharasaki, Mamoru, Sin, Della, Wang, Jing

“…An international CCQM-P60 pilot study involving eight national metrological institutes was organized to investigate if the quantification of genetically…”
Get full text

Dissipation of the membrane potential in susceptible corn mitochondria by the toxin of Helminthosporium maydis, race T, and toxin analogs by Holden, M.J, Sze, H

“…We have tested directly the effect of Helminthosporium maydis T (Hmt) toxin and various analogs on the membrane potential formed in mitochondria isolated from…”
Get full text

Sensitivity comparison of real-time PCR probe designs on a model DNA plasmid by Wang, L., Blasic, J.R., Holden, M.J., Pires, R.

“…We investigated three probe design strategies used in quantitative polymerase chain reaction (PCR) for sensitivity in detection of the PCR amplicon. A plasmid…”
Get full text

Helminthosporium maydis T toxin increased membrane permeability to Ca2+ [calcium ion] in susceptible corn mitochondria [Maize, leaf blight] by Holden, M.J, Sze, H

Get full text

Spectroscopic characterization of fluorescein- and tetramethylrhodamine-labeled oligonucleotides and their complexes with a DNA template by Wang, L., Gaigalas, A.K., Blasic, J., Holden, M.J.

“…We measured absorption and emission spectra, fluorescence quantum yield, anisotropy, fluorescence resonance energy transfer (FRET), and melting temperature to…”
Get full text

The outer mitochondrial membrane channel, VDAC, is modulated by a protein localized in the intermembrane space by Holden, M J, Colombini, M

“…The mitochondrial outer membrane channel, VDAC, provides a pathway for the flux of metabolites between the cytoplasm and mitochondrion. VDAC is…”
Get more information

Fe3+-chelate reductase activity of plasma membranes isolated from tomato (Lycopersicon esculentum Mill.) roots: Comparison of enzymes from Fe-deficient and Fe-sufficient roots by HOLDEN, M. J, LUSTER, D. G, CHANEY, R. L, BUCKHOUT, T. J, ROBINSON, C

“…Reduction of Fe3+ to Fe2+ is a prerequisite for Fe uptake by tomato roots. Ferric chelate reductase activity in plasma membranes (PM) isolated from roots of…”
Get full text

Chorismate lyase: kinetics and engineering for stability by Holden, M.J., Mayhew, M.P., Gallagher, D.T., Vilker, V.L.

“…By removing the enolpyruvyl group from chorismate, chorismate lyase (CL) produces p-hydroxybenzoate ( p-HB) for the ubiquinone biosynthetic pathway. We have…”
Get full text

Cu2+ reduction by tomato root plasma membrane vesicles by Holden, M.J. (National Institute of Standards and Technology, Gaithersburg, MD.), Crimmins, T.J. Jr, Chaney, R.L

“…Reduction of Cu2+ by plasma membrane vesicles isolated from tomato (Lycopersicon esculentum Mill.) roots was investigated. Plants were grown in hydroponic…”
Get full text

Effects of Helminthosporium maydis Race T Toxin on Electron Transport in Susceptible Corn Mitochondria and Prevention of Toxin Actions by Dicyclohexylcarbodiimide by Marcia J. Holden, Sze, Heven

“…The effect of Helminthosporium maydis race T toxin on electron transport in susceptible cytoplasmic male-sterile Texas corn (Zea mays L.) mitochondria was…”
Get full text

Effects of Helminthosporium maydis rate T toxin on electron transport in susceptible corn mitochondria and prevention of toxin actions by dicyclohexylcarbodiimide by Holden, M.J. (USDA, ARS, Foreign Disease-Weed Science, Ft. Detrick, Frederick, MD), Sze, H

“…The effect of Helminthosporium maydis race T toxin on electron transport in susceptible cytoplasmic male-sterile Texas corn (Zea mays L.) mitochondria was…”
Get full text

Channel formation in phospholipid bilayer membranes by the toxin of Helminthosporium maydis, race T by Holden, M.J, Colombini, M, Sze, H

“…Southern Corn Leaf Blight is caused by a toxin produced by a virulent form of Helminthosporium maydis (Race T). The toxin has been shown to uncouple oxidative…”
Get full text

Taxonomic implication of sterol composition in the genus Chlorella [Green algae] by Holden, Marcia J., Patterson, Glenn W.

“…Abstract Thirty‐five isolates of the genus Chlorella were grown under standardized conditions and analyzed for sterol composition. Six different sterol…”
Get full text

Gathering requirements from remote users by Leonard, T., Berzins, V., Holden, M.J.

“…We describe a distributed requirements engineering environment using computer aided software engineering tools linked together through the Internet. We created…”
Get full text