On the formation of protein corona on colloidal nanoparticles stabilized by depletant polymers

To counter the undesired colloidal destabilization of nanoparticles in biologically-compatible media of high ionic strength (i.e. NaCl, phosphate buffer), polymers can be added to nanoparticle suspensions that will be used in biomedical applications. In these suspensions, polymers can promote high c...

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Bibliographic Details
Published in:	Materials Science & Engineering C Vol. 105; p. 110080
Main Authors:	Petry, Romana, Saboia, Viviane M., Franqui, Lidiane S., Holanda, Camila de A., Garcia, Thiago R.R., de Farias, Marcelo A., de Souza Filho, Antonio G., Ferreira, Odair P., Martinez, Diego S.T., Paula, Amauri J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2019 Elsevier BV
Subjects:	Addition polymerization Animals Biocompatibility Biomedical materials Biomolecules Blood plasma Bovine serum albumin Buffers Cattle Colloids Depletion Destabilization Human blood plasma Humans Ionic strength Materials science Nanoparticles Nanoparticles - chemistry Phosphates Pluronic F-127 Poloxamer - chemistry Poly(ethylene glycol) Polyethylene glycol Polyethylene Glycols - chemistry Polymers Protein composition Protein corona Protein Corona - chemistry Proteins Repulsive depletion forces Serum albumin Serum Albumin, Bovine - chemistry Silica Silicon dioxide Silicon Dioxide - chemistry Sodium chloride Stability Stabilizers (agents) Stöber silica nanoparticles Bovine serum albumin Poly(ethylene glycol) Human blood plasma Repulsive depletion forces Pluronic F-127 Protein corona Stöber silica nanoparticles
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Summary:	To counter the undesired colloidal destabilization of nanoparticles in biologically-compatible media of high ionic strength (i.e. NaCl, phosphate buffer), polymers can be added to nanoparticle suspensions that will be used in biomedical applications. In these suspensions, polymers can promote high colloidal stability by manifestation of steric and/or depletion forces. However, little is known about the influence of these polymers on the interactions between nanoparticles and the biological components of the organism, such as proteins and cells. In this work, it was shown that the addition of the polymers (i) Pluronic-F127 (PF127), (ii) polyethylene glycol (PEG) of different molecular weights – 1.5, 12 and 35 kDa – and (iii) the protein bovine serum albumin (BSA) on colloidal silica nanoparticles (CSNPs; 135 nm) dispersed in phosphate-buffered saline (PBS) largely alter their colloidal stability through different mechanisms. Although all polymers were adsorbed on the CSNP surface, BSA maintained the CSNP dispersion in the medium by electrosteric stabilization mechanisms, while PEG and PF127 led to the occurrence of depletion forces between the particles. In addition, it was found that the interactions between polymers and CSNPs did not prevent proteins to access the nanoparticles' surface and have minimal effect on the formation of the protein corona when they were incubated in human blood plasma. On the other hand, BSA had a greater effect on the CSNP protein corona profile compared to other polymers (PEG and PF127). Together, these results confirm that biocompatible polymers PEG and PF127 can be used as colloidal stabilizing agents for nanoparticles since they preserve the accessibility of biomolecules to the nanoparticle surface, and they have little effect on the protein corona composition. [Display omitted] •PEG and PF127 create depletion forces in colloids of silica nanoparticles (SCNPs) dispersed in media of high ionic strength.•Depletion forces manifested by PF127 can promote colloidal stabilization of nanoparticles in physiological medium (PBS).•Although PEG and PF127 adsorb on the SCNPs surfaces, plasma proteins replace these polymers in a later adsorption.•SCNPs suspension stabilized with PEG and PF127 by depletion forces did not have the protein corona composition altered.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0928-4931 1873-0191
DOI:	10.1016/j.msec.2019.110080