Search Results - "Hogrefe, H H"

Amplification efficiency of thermostable DNA polymerases by Arezi, Bahram, Xing, Weimei, Sorge, Joseph A, Hogrefe, Holly H

“…The amplification efficiencies of several polymerase chain reaction (PCR) enzymes were compared using real-time quantitative PCR with SYBR Green I detection…”
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PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA Polymerases by Cline, Janice, Braman, Jeffery C., Hogrefe, Holly H.

“…The replication fidelities of Pfu, Taq, Vent, Deep Vent and UlTma DNA polymerases were compared using a PCR-based forward mutation assay. Average error rates…”
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Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit by Hogrefe, Holly H, Cline, Janice, Youngblood, Geri L, Allen, Ronda M

“…The QuikChange Multi Site-Directed Mutagenesis Kit is a simple and efficient method for introducing point mutations at up to five sites simultaneously in…”
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Archaeal dUTPase Enhances PCR Amplifications with Archaeal DNA Polymerases by Preventing dUTP Incorporation by Hogrefe, Holly H., Hansen, Connie J., Scott, Bradley R., Nielson, Kirk B.

“…We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A…”
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Rapid quantification of DNA libraries for next-generation sequencing by Buehler, Bernd, Hogrefe, Holly H., Scott, Graham, Ravi, Harini, Pabón-Peña, Carlos, O’Brien, Scott, Formosa, Rachel, Happe, Scott

“…The next-generation DNA sequencing workflows require an accurate quantification of the DNA molecules to be sequenced which assures optimal performance of the…”
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Efficient and High Fidelity Incorporation of Dye-terminators by a Novel Archaeal DNA Polymerase Mutant by Arezi, Bahram, Hansen, Connie J, Hogrefe, Holly H

“…We examined the molecular basis of ddNTP selectivity in archaeal family B DNA polymerases by randomly mutagenizing the gene encoding Thermococcus sp. JDF-3 DNA…”
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Escherichia coli DNA polymerase III ε subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT–PCR procedures by Arezi, Bahram, Hogrefe, Holly H.

“…In an effort to improve reverse transcriptase (RT) fidelity, we measured the error rate of Moloney murine leukemia virus (MMLV) RT in the presence of several…”
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Outcomes einer stationären multimodalen Komplexbehandlung des Bewegungssystems: Ergebnisse der Pilotphase zur ANOA-Studie by Derlien, S., Danckwerth, F., Alfter, S., Henning, J., Hogrefe, H.-C., Holtschmit, J. H., Jungbluth, A., Lang, C., Menke, J., Olsen, G., Pioch, E., Psczolla, M., Seidel, W., Smolenski, U. C., Thümmel, J., Niemier, K.

“…Zusammenfassung Hintergrund Chronische Schmerzsyndrome des Bewegungssystems sind ein bedeutender Kostenfaktor. Trotz dieser Ausgaben sind die…”
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DNA polymerases from hyperthermophiles by Hogrefe, H H, Cline, J, Lovejoy, A E, Nielson, K B

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Kinetic analysis of Escherichia coli RNase H using DNA-RNA-DNA/DNA substrates by Hogrefe, H H, Hogrefe, R I, Walder, R Y, Walder, J A

“…The kinetic properties of Escherichia coli ribonuclease H (RNase H) were investigated using oligonucleotide substrates that consist of a short stretch of RNA,…”
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Identification of Human Antibody Fragment Clones Specific for Tetanus Toxoid in a Bacteriophage λ Immunoexpression Library by Mullinax, Rebecca L., Gross, Eleanor A., Amberg, Jeff R., Hay, Beverly N., Hogrefe, Holly H., Kubitz, Marjorie M., Greener, Alan, Alting-Mees, Michele, Ardourel, Daniel, Short, Jay M., Sorge, Joseph A., Shopes, Bob

“…We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA…”
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Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution by Kiefer, J R, Mao, C, Hansen, C J, Basehore, S L, Hogrefe, H H, Braman, J C, Beese, L S

“…The study of DNA polymerases in the Pol l family is central to the understanding of DNA replication and repair. DNA polymerases are used in many molecular…”
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Positionierung der Osteopathie zur manuellen Medizin by Hogrefe, H.-C., Rings, V., Smolenski, U.C.

“…Die Osteopathie bzw. osteopathische Medizin ist in ihrem Heimatland, den USA, und nicht nur vonseiten der DGOM und des EROP auf dem europäischen Kontinent…”
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Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide gamma-phosphate derivative by Hansen, Connie J, Wu, Lydia, Fox, Jeffrey D, Arezi, Bahram, Hogrefe, Holly H

“…Using compartmentalized self-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates modification of the γ-phosphate…”
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Characterization of the antigenic sites on the refined 3-A resolution structure of mouse testicular lactate dehydrogenase C4 by Hogrefe, H H, Griffith, J P, Rossmann, M G, Goldberg, E

“…The atomic structure of mouse testicular apolactate dehydrogenase C4 has been refined to 3.0-A resolution yielding a final crystallographic R-factor of 0.256…”
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Immunogenicity of Synthetic Peptides Corresponding to Flexible and Antibody-accessible Segments of Mouse Lactate Dehydrogenase (LDH)-C4 by Hogrefe, H H, Kaumaya, P T P, Goldberg, E

“…The immunological properties of a panel of synthetic peptides that represent the most accessible and mobile segments of the lactate dehydrogenase (LDH)-C4…”
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Amino Terminus Is Essential to the Structural Integrity of Recombinant Human Interferon-γ by Hogrefe, H H, McPhie, P, Bekisz, J B, Enterline, J C, Dyer, D, Webb, D S A, Gerrard, T L, Zoon, K C

“…Species lacking either 8 or 10 residues at the amino terminus of recombinant human interferon-γ (Hu-IFN-γ) were generated by limited digestion with…”
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Die Muskulatur bei der Untersuchung und Behandlung aus Sicht der DGOM by Hogrefe, H.-C.

“…Andrew Still erkannte in der Muskulatur einen wesentlichen manualtherapeutischen Zugangsweg zur Diagnosestellung und Behandlung von Funktionsstörungen. Die…”
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Construction of phagemid display libraries with PCR-amplified immunoglobulin sequences by Hogrefe, H H, Shopes, B

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A bacteriophage lambda vector for the cloning and expression of immunoglobulin Fab fragments on the surface of filamentous phage by Hogrefe, H H, Mullinax, R L, Lovejoy, A E, Hay, B N, Sorge, J A

“…We have combined the high cloning efficiency of the lambda bacteriophage vectors with the surface expression screening method for the display of combinatorial…”
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