CHEMICAL COMPOSITION OF ESSENTIAL OILS OF EUGENIA CARYOPHYLLA AND MENTHA SP CF PIPERITA AND THEIR IN VITRO ANTIFUNGAL ACTIVITIES ON SIX HUMAN PATHOGENIC FUNGI
Background: Many fungal infections are responsible for human skin damages, to control their negative action, some aromatic and medicinal plants are traditionally used by local population in Cameroon. The present study was carried out to determine the chemical composition of essential oils of Eugenia...
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| Published in: | African journal of traditional, complementary, and alternative medicines Vol. 11; no. 6; pp. 40 - 46 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
African Ethnomedicines Network
09-04-2015
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Background: Many fungal infections are responsible for human skin
damages, to control their negative action, some aromatic and medicinal
plants are traditionally used by local population in Cameroon. The
present study was carried out to determine the chemical composition of
essential oils of Eugenia caryophylla and Mentha sp cf piperita and
their antifungal activity on some human pathogenic fungi. Materials and
Methods: Essential oils from Eugenia caryophylla and Mentha sp cf
piperita were extracted by steam distillation using Clevenger apparatus
and the antifungal activity was evaluated on six human pathogenic
fungal strains; two yeasts ( Candida albicans 1 and Candida albicans
2) and four dermatophytes ( Tricophyton rubrum 1, T. rubrum 2, T.
violaceum, and T. soudanensis) using modified broth microdilution
method M27-A3 and M38-A respectively. Results: The essential oils
obtained yielded of 5.9 for Eugenia caryophylla and 0.2% Mentha sp cf
piperita respectively. The chemical composition was assigned by GC and
GC/SM and showed that E. caryophylla was mainly composed of eugenol
(80.0 %), β-caryophyllene (8.3%), and eugenol acetate (6.7%) while
Mentha sp cf piperita was characterized by piperitone (67.5 %), menthol
(10.0 %) and β-phellandrene (5.8%). The result showed that
essential oil of E. caryophylla exhibit the highest antifungal activity
with MICs and MFC of 0.25μgL/mL and 0.125μgL/mL for
filamentous fungi and MIC of 0.5 μgL/mL for both yeast strains
while MFC value was 1 μgL/mL for one yeast strain and not
determined for the second. MFCs Mentha sp cf piperita essential oil
showed a weak activity with a MIC of 2.5 μgL/mL on Tricophyton
strains while no activity was exhibited on Candida albicans strains.
Conclusion: The results of this work can be used to confirm their
traditional uses and can also be proposed as natural ingredients to
some industries to treat superficial infections. |
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| ISSN: | 0189-6016 0189-6016 |
| DOI: | 10.4314/ajtcam.v11i6.3 |