Autocrine growth effect of IL-8 and GROalpha on a human pancreatic cancer cell line, Capan-1

A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROalpha). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Gialpha-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or a...

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Bibliographic Details
Published in:	Pancreas Vol. 21; no. 1; pp. 52 - 56
Main Authors:	Takamori, H, Oades, Z G, Hoch, O C, Burger, M, Schraufstatter, I U
Format:	Journal Article
Language:	English
Published:	United States 01-07-2000
Subjects:	Antibodies, Monoclonal - pharmacology Cell Division - drug effects Chemokine CXCL1 Chemokines, CXC Chemotactic Factors - genetics Chemotactic Factors - physiology Growth Inhibitors - genetics Growth Inhibitors - physiology Growth Substances - genetics Growth Substances - physiology Humans Intercellular Signaling Peptides and Proteins Interleukin-8 - genetics Interleukin-8 - physiology Pancreatic Neoplasms Pertussis Toxin Receptors, Interleukin-8B - genetics Transcription, Genetic Tumor Cells, Cultured Virulence Factors, Bordetella - pharmacology
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Summary:	A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROalpha). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Gialpha-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROalpha monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Gialpha also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROalpha act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0885-3177