Transport Form and Pathway from the Intestine to the Peripheral Tissues and the Intestinal Absorption and Metabolism Properties of Oleamide

This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation...

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Bibliographic Details
Published in:	Journal of agricultural and food chemistry Vol. 70; no. 49; pp. 15499 - 15508
Main Authors:	Kobayashi, Yasuyuki, Watanabe, Natsumi, Hiura, Reina, Kubota, Mai, Furuta, Kousuke, Sugimoto, Keiichiro, Murota, Kaeko, Nakamura, Eri, Matsuura, Toshiki, Kai, Kenji, Inui, Takashi, Kitakaze, Tomoya, Harada, Naoki, Yamaji, Ryoichi
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 14-12-2022
Subjects:	Bioactive Constituents, Metabolites, and Functions oleamide lymphatic vessel portal vein fatty acid amide hydrolase CD36 bioavailability
Online Access:	Get full text
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Summary:	This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation indicated that plasma oleamide was enriched in the fractions containing albumin in the portal and peripheral blood. Oleamide formed a complex with albumin in an endothermic reaction (apparent K d = 4.4 μM). The CD36 inhibitor inhibited the oleamide uptake into the intestinal epithelial Caco-2 cells, and oleamide decreased the cell surface CD36 level. The fatty acid amide hydrolase (FAAH) inhibitor increased the transepithelial transport of oleamide across Caco-2 cells and the plasma oleamide concentration in mice intragastrically administered with oleamide. These results indicate that oleamide is transported primarily via the portal vein as a complex with albumin. Furthermore, we suggest that oleamide is taken up via CD36 in the small intestine and degraded intracellularly by FAAH.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-8561 1520-5118
DOI:	10.1021/acs.jafc.2c06791