Multiple effects on peripheral hematology following administration of recombinant human interleukin 12 to nonhuman primates

Multiple effects on peripheral hematology following administration of recombinant human interleukin 12 to nonhuman primates

Interleukin 12 has demonstrated a wide spectrum of bioactivity on T and NK cells both in vitro and in vivo. Therapeutic potential of these activities has been suggested by studies in murine tumor, viral and microbial models of disease. We have investigated the in vivo effect of rhlL-12 in cynomolgus...

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Bibliographic Details
Published in:Biochemical and biophysical research communications Vol. 204; no. 3; p. 1150
Main Authors: Bree, A G, Schlerman, F J, Kaviani, M D, Hastings, R C, Hitz, S L, Goldman, S J
Format: Journal Article
Language:English
Published: United States 15-11-1994
Subjects:
Animals
Biopterins - analogs & derivatives
Biopterins - blood
Hematocrit
Immunophenotyping
Injections, Intravenous
Injections, Subcutaneous
Interleukin-12 - administration & dosage
Interleukin-12 - pharmacology
Leukocyte Count - drug effects
Lymphocyte Count - drug effects
Lymphocyte Subsets - drug effects
Lymphocyte Subsets - immunology
Macaca fascicularis
Male
Monocytes - cytology
Monocytes - drug effects
Monocytes - immunology
Neopterin
Platelet Count - drug effects
Recombinant Proteins - administration & dosage
Recombinant Proteins - pharmacology
Reference Values
Time Factors
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Summary:Interleukin 12 has demonstrated a wide spectrum of bioactivity on T and NK cells both in vitro and in vivo. Therapeutic potential of these activities has been suggested by studies in murine tumor, viral and microbial models of disease. We have investigated the in vivo effect of rhlL-12 in cynomolgus monkeys treated with 1 micrograms/kg/day by bolus i.v. or s.c. injection for 5 days. Treated group transient decreases in total WBC counts as compared to controls, with reversable decreases seen mainly in the lymphocyte and monocyte subsets. Phenotypic analysis showed a decrease in the number of CD4+ and CD8+ cells/microL on Days 2 and 4. Reversible thrombocytopenia and anemia were noted in both treated groups as compared to controls. Plasma neopterin concentrations were increased in both treated groups as compared to controls. These data show that rhlL-12 has multiple effects on peripheral hematology and suggests that this model may be useful to investigate in vivo bioactivity of rhlL-12.
ISSN:0006-291X
DOI:10.1006/bbrc.1994.2583