Search Results - "Hisatake, Koji"

Mechanisms of the Metabolic Shift during Somatic Cell Reprogramming by Nishimura, Ken, Fukuda, Aya, Hisatake, Koji

“…Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold a huge promise for regenerative medicine,…”
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A Sendai virus-based expression system directs efficient induction of chondrocytes by transcription factor-mediated reprogramming by Zhou, Jingwen, Sekiguchi, Yuya, Sano, Masayuki, Nishimura, Ken, Hisatake, Koji, Fukuda, Aya

“…Cartilage rarely heals spontaneously once damaged. Osteoarthritis (OA) is the most common degenerative joint disease among the elderly; however, effective…”
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Locus-Specific Isolation of the Nanog Chromatin Identifies Regulators Relevant to Pluripotency of Mouse Embryonic Stem Cells and Reprogramming of Somatic Cells by Burramsetty, Arun Kumar, Nishimura, Ken, Kishimoto, Takumi, Hamzah, Muhammad, Kuno, Akihiro, Fukuda, Aya, Hisatake, Koji

“…Pluripotency is a crucial feature of pluripotent stem cells, which are regulated by the core pluripotency network consisting of key transcription factors and…”
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Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator by Takei, Yuki, Hirai, Rie, Fukuda, Aya, Miyazaki, Shinichi, Shimada, Rintaro, Okamatsu-Ogura, Yuko, Saito, Masayuki, Leproux, Philippe, Hisatake, Koji, Kano, Hideaki

“…We visualized a dynamic process of fatty acid uptake of brown adipocytes using a time-lapse ultra-broadband multiplex coherent anti-Stokes Raman scattering…”
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BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence by Hayashi, Yohei, Hsiao, Edward C., Sami, Salma, Lancero, Mariselle, Schlieve, Christopher R., Nguyen, Trieu, Yano, Koyori, Nagahashi, Ayako, Ikeya, Makoto, Matsumoto, Yoshihisa, Nishimura, Ken, Fukuda, Aya, Hisatake, Koji, Tomoda, Kiichiro, Asaka, Isao, Toguchida, Junya, Conklin, Bruce R., Yamanaka, Shinya

“…Fibrodysplasia ossificans progressiva (FOP) patients carry a missense mutation in ACVR1 [617G > A (R206H)] that leads to hyperactivation of BMP-SMAD signaling…”
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Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells by Nishimura, Ken, Ishiwata, Hiroshi, Sakuragi, Yuta, Hayashi, Yohei, Fukuda, Aya, Hisatake, Koji

“…Pluripotent stem cells (PSCs) have various degrees of pluripotency, which necessitates selection of PSCs with high pluripotency before their application to…”
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Downregulation of Odd-Skipped Related 2, a Novel Regulator of Epithelial-Mesenchymal Transition, Enables Efficient Somatic Cell Reprogramming by Anh, Le Phuong Hoang, Nishimura, Ken, Kuno, Akihiro, Linh, Nguyen Thuy, Kato, Tetsuo, Ohtaka, Manami, Nakanishi, Mahito, Sugihara, Eiji, Sato, Taka-Aki, Hayashi, Yohei, Fukuda, Aya, Hisatake, Koji

“…Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). The early stage of reprogramming of mouse…”
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An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors by Kishimoto, Takumi, Nishimura, Ken, Morishita, Kana, Fukuda, Aya, Miyamae, Yusaku, Kumagai, Yutaro, Sumaru, Kimio, Nakanishi, Mahito, Hisatake, Koji, Sano, Masayuki

“…Viral vectors are attractive gene delivery vehicles because of their broad tropism, high transduction efficiency, and durable expression. With no risk of…”
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Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302 by Nishimura, Ken, Ohtaka, Manami, Takada, Hitomi, Kurisaki, Akira, Tran, Nhi Vo Kieu, Tran, Yen Thi Hai, Hisatake, Koji, Sano, Masayuki, Nakanishi, Mahito

“…Transgene-free induced pluripotent stem cells (iPSCs) are valuable for both basic research and potential clinical applications. We previously reported that a…”
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Factors Affecting Undergraduate Medical Science Students’ Motivation to Study during the COVID-19 Pandemic by Mayers, Thomas, Mathis, Bryan J., Ho, C. Kiong, Morikawa, Kazuya, Maki, Naoki, Hisatake, Koji

“…In the current study, we investigated the motivational status and underlying factors of the motivational changes among Japanese medical science students…”
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Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720 by Fukuda, Aya, Honda, Shiho, Fujioka, Norie, Sekiguchi, Yuya, Mizuno, Seiya, Miwa, Yoshihiro, Sugiyama, Fumihiro, Hayashi, Yohei, Nishimura, Ken, Hisatake, Koji

“…Uncoupling protein 1 (UCP1) is a mitochondrial protein that is expressed in both brown and beige adipocytes. UCP1 uncouples the mitochondrial electron…”
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Essential Role of p38 Mitogen-activated Protein Kinase in Cathepsin K Gene Expression during Osteoclastogenesis through Association of NFATc1 and PU.1 by Matsumoto, Masahito, Kogawa, Masakazu, Wada, Seiki, Takayanagi, Hiroshi, Tsujimoto, Masafumi, Katayama, Shigehiro, Hisatake, Koji, Nogi, Yasuhisa

“…The receptor activator of NF-κB ligand (RANKL) induces various osteoclast-specific marker genes during osteoclast differentiation mediated by…”
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Utilization of a novel Sendai virus vector in ex vivo gene therapy for hemophilia A by Yamaki, Yuni, Fukushima, Takashi, Yoshida, Naomi, Nishimura, Ken, Fukuda, Aya, Hisatake, Koji, Aso, Masayuki, Sakasai, Tomoki, Kijima-Tanaka, Junko, Miwa, Yoshihiro, Nakanishi, Mahito, Sumazaki, Ryo, Takada, Hidetoshi

“…Sendai virus (SeV) vectors are being recognized as a superior tool for gene transfer. Here, we report the transfection efficacy of a novel, high-performance,…”
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DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation by Chen, Yexi, Yamaguchi, Yuki, Tsugeno, Yuta, Yamamoto, Junichi, Yamada, Tomoko, Nakamura, Mitsuhiro, Hisatake, Koji, Handa, Hiroshi

“…Transcription elongation factor DSIF/Spt4-Spt5 is capable of promoting and inhibiting RNA polymerase II elongation and is involved in the expression of various…”
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Template Activating Factor-I α Regulates Retroviral Silencing during Reprogramming by Bui, Phuong Linh, Nishimura, Ken, Seminario Mondejar, Gonzalo, Kumar, Arun, Aizawa, Shiho, Murano, Kensaku, Nagata, Kyosuke, Hayashi, Yohei, Fukuda, Aya, Onuma, Yasuko, Ito, Yuzuru, Nakanishi, Mahito, Hisatake, Koji

“…Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by dramatic changes in epigenetic programs, including silencing of…”
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Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming by Aizawa, Shiho, Nishimura, Ken, Mondejar, Gonzalo Seminario, Kumar, Arun, Bui, Phuong Linh, Tran, Yen Thi Hai, Kuno, Akihiro, Muratani, Masafumi, Kobayashi, Shin, Nabekura, Tsukasa, Shibuya, Akira, Sugihara, Eiji, Sato, Taka-Aki, Fukuda, Aya, Hayashi, Yohei, Hisatake, Koji

“…Reprogramming of murine female somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by X chromosome reactivation (XCR), by which the inactive…”
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A Role for KLF4 in Promoting the Metabolic Shift via TCL1 during Induced Pluripotent Stem Cell Generation by Nishimura, Ken, Aizawa, Shiho, Nugroho, Fransiska Liliani, Shiomitsu, Emi, Tran, Yen Thi Hai, Bui, Phuong Linh, Borisova, Evgeniia, Sakuragi, Yuta, Takada, Hitomi, Kurisaki, Akira, Hayashi, Yohei, Fukuda, Aya, Nakanishi, Mahito, Hisatake, Koji

“…Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is accompanied by morphological, functional, and metabolic alterations before…”
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Manipulation of KLF4 Expression Generates iPSCs Paused at Successive Stages of Reprogramming by Nishimura, Ken, Kato, Tetsuo, Chen, Chen, Oinam, Lalhaba, Shiomitsu, Emi, Ayakawa, Daisuke, Ohtaka, Manami, Fukuda, Aya, Nakanishi, Mahito, Hisatake, Koji

“…The detailed mechanism of reprogramming somatic cells into induced pluripotent stem cells (iPSCs) remains largely unknown. Partially reprogrammed iPSCs are…”
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Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene by Fukuda, Aya, Shimada, Miho, Nakadai, Tomoyoshi, Nishimura, Ken, Hisatake, Koji

“…The c-fos gene responds to extracellular stimuli and undergoes robust but transient transcriptional activation. Here we show that heterogeneous nuclear…”
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Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency by Borisova, Evgeniia, Nishimura, Ken, An, Yuri, Takami, Miho, Li, Jingyue, Song, Dan, Matsuo-Takasaki, Mami, Luijkx, Dorian, Aizawa, Shiho, Kuno, Akihiro, Sugihara, Eiji, Sato, Taka-aki, Yumoto, Fumiaki, Terada, Tohru, Hisatake, Koji, Hayashi, Yohei

“…Non-genetically modified somatic cells can only be inefficiently and stochastically reprogrammed to pluripotency by exogenous expression of reprogramming…”
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