Distinct metabolites for photoreactive L-phenylalanine derivatives in Klebsiella sp. CK6 isolated from rhizosphere of a wild dipterocarp sapling

Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores--aryl azide, benzophenone and trifluoromethyldiazirine--are utilized in analysis. Photophore-bearing L-phenylalanine derivatives, which are used for biological functional analysis, were...

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Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Vol. 18; no. 7; pp. 8393 - 8401
Main Authors:	Wang, Lei, Hisano, Wataru, Murai, Yuta, Sakurai, Munenori, Muto, Yasuyuki, Ikemoto, Haruka, Okamoto, Masashi, Murotani, Takashi, Isoda, Reika, Kim, Dongyeop, Sakihama, Yasuko, Sitepu, Irnayuli R, Hashidoko, Yasuyuki, Hatanaka, Yasumaru, Hashimoto, Makoto
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 16-07-2013 MDPI
Subjects:	Amino acids benzophenone Benzophenones - chemistry Dipterocarpaceae - microbiology Klebsiella - classification Klebsiella - isolation & purification Klebsiella - metabolism Labeling Metabolism Metabolites Nitrogen phenyl azide phenylalanine Phenylalanine - analogs & derivatives Phenylalanine - chemistry Phenylalanine - metabolism photoaffinity label Photoaffinity Labels R&D Research & development Rhizosphere trifluoromethyldiazirine tryptophan Tryptophan - chemistry Tryptophan - metabolism Japan Indonesia
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Summary:	Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores--aryl azide, benzophenone and trifluoromethyldiazirine--are utilized in analysis. Photophore-bearing L-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogen-limiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing L-phenylalanine derivatives.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules18078393