Search Results - "Hisada, Akiko"
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Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy
Published in Scientific reports (12-07-2023)“…Rapid determination of drug efficacy against bacterial pathogens is needed to detect potentially resistant bacteria and allow for more rational use of…”
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Antimicrobial Susceptibility Testing for Colistin: Extended Application of Novel Quantitative and Morphologic Assay Using Scanning Electron Microscopy
Published in International journal of microbiology (25-05-2024)“…Background. Colistin (Polymyxin E) has reemerged in the treatment of MDR Gram-negative infections. Traditional Colistin AST methods have long turnaround times…”
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Rapid Detection of Imipenem Resistance in Gram-Negative Bacteria Using Tabletop Scanning Electron Microscopy: A Preliminary Evaluation
Published in Frontiers in microbiology (16-06-2021)“…Background: Enabling faster Antimicrobial Susceptibility Testing (AST) is critical, especially to detect antibiotic resistance, to provide rapid and…”
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Rapid microbial viability assay using scanning electron microscopy: a proof-of-concept using Phosphotungstic acid staining
Published in Computational and structural biotechnology journal (01-01-2023)“…Multiple stains have been historically utilized in electron microscopy to provide proper contrast and superior image quality enabling the discovery of…”
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Antimicrobial susceptibility testing for Gram positive cocci towards vancomycin using scanning electron microscopy
Published in Current research in microbial sciences (01-01-2022)“…•SEM-based method can be applied for rapid phenotypic AST on Gram-positive cocci towards 2 vancomycin based on morphological changes 3.•The ratio of septa as a…”
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A Pt layer/Pt disk electrode configuration to evaluate respiration and alkaline phosphatase activities of mouse embryoid bodies
Published in Talanta (Oxford) (30-05-2012)“…[Display omitted] ► An integrated probe was fabricated, with working/pseudo reference electrode. ► We measured oxygen consumption and alkaline phosphatase…”
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A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis
Published in The Plant journal : for cell and molecular biology (01-02-2002)“…Summary The plant vacuole is a multifunctional organelle which is essential for growth and development. To visualize the dynamics of plant vacuolar membranes,…”
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Differential Effect of Scaffold Shape on Dentin Regeneration
Published in Annals of biomedical engineering (01-04-2010)“…The effect of scaffold shape on dentin regeneration is not well understood. In this study, porous hydroxyapatite/beta-tricalcium phosphate (HAp/β-TCP),…”
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Noninvasively measuring respiratory activity of rat primary hepatocyte spheroids by scanning electrochemical microscopy
Published in Journal of bioscience and bioengineering (01-01-2014)“…Construction of an in vitro drug screening method for evaluating drug metabolism and toxicity by using cells is required instead of the conventional in vivo…”
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Light-Induced Nuclear Translocation of Endogenous Pea Phytochrome A Visualized by Immunocytochemical Procedures
Published in The Plant cell (01-07-2000)“…Although the physiological functions of phytochrome A (PhyA) are now known, the distribution of endogenous PhyA has not been examined. We have visualized…”
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Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity
Published in Journal of clinical microbiology (01-10-2019)“…We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the…”
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Electron tomography of whole cultured cells using novel transmission electron imaging technique
Published in Micron (Oxford, England : 1993) (01-01-2018)“…•Plate-TEM tomography was developed for 3D observation of cellular structures.•The structures can be observed at high resolution, as cells remain on a…”
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Technical advance: An automated device for cryofixation of specimens of electron microscopy using liquid helium
Published in Plant and cell physiology (01-09-2001)“…Metal-contact rapid freezing using liquid helium is theoretically the best method for preserving the fine structure of living cells with high temporal…”
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Formation of hepatocyte spheroids with structural polarity and functional bile canaliculi using nanopillar sheets
Published in Tissue engineering. Part A (01-06-2010)“…We developed a method for controlling the spheroid formation of adult rat primary hepatocytes simply by optimizing the pillar diameters and patterns of…”
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Phytochrome A requires jasmonate for photodestruction
Published in Planta (01-04-2009)“…The plant photoreceptor phytochrome is organised in a small gene family with phytochrome A (phyA) being unique, because it is specifically degraded upon…”
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Deep Learning Based Bacteria Classification from SEM Images Using a Combination of Membrane and Internal Features
Published in 2022 56th Annual Conference on Information Sciences and Systems (CISS) (09-03-2022)“…Scanning electron microscopes (SEM) take very high-magnification images that allow us to examine the mor-phological features of bacteria samples in great…”
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Conference Proceeding -
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Quantitative Analysis System for Bacterial Cells in SEM Image using Deep Learning
Published in 2021 55th Annual Conference on Information Sciences and Systems (CISS) (24-03-2021)“…In this paper we propose a system to analyze bacteria from a given Scanning Electron Microscope (SEM) image of the bacterial sample. Thousands of bacteria…”
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Conference Proceeding -
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Light-induced nuclear transolcation of endogenous pea phytochrome A visualized by immunocytochemical procedures
Published in The Plant cell (01-07-2000)“…Although the physiological functions of phytochrome A (PhyA) are now known, the distribution of endogenous PhyA has not been examined. We have visualized…”
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Journal Article -
19
Technical Advance: An Automated Device for Cryofixation ofSpecimens of Electron Microscopy using LiquidHelium
Published in Plant and cell physiology (15-09-2001)“…Metal-contact rapid freezing using liquid helium is theoretically the best method for preserving the fine structure of living cells with high temporal…”
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Journal Article -
20
Light-Induced Nuclear Translocation of Endogenous Pea Phytochrome A Visualized by Immunocytochemical Procedures
Published in The Plant cell (01-07-2000)Get full text
Journal Article