Search Results - "Hiroyuki Sunagawa"

In Vitro Reconstitution of the Clostridium botulinum Type D Progenitor Toxin by Kouguchi, Hirokazu, Watanabe, Toshihiro, Sagane, Yoshimasa, Sunagawa, Hiroyuki, Ohyama, Tohru

“…Clostridium botulinum type D strain 4947 produces two different sizes of progenitor toxins (M and L) as intact forms without proteolytic processing. The M…”
Get full text

Protective Effects of Quercetin and Its Metabolites on H2O2-Induced Chromosomal Damage to WIL2-NS Cells by SAITO, Akiko, SUGISAWA, Ayako, UMEGAKI, Keizo, SUNAGAWA, Hiroyuki

“…We investigated chromosomal damage caused by a typical flavonoid, quercetin, and its two conjugates, quercetin-3-O-sulfate and isorhamnetin, and their…”
Get full text

Spontaneous Nicking in the Nontoxic–Nonhemagglutinin Component of the Clostridium botulinum Toxin Complex by Sagane, Yoshimasa, Watanabe, Toshihiro, Kouguchi, Hirokazu, Sunagawa, Hiroyuki, Obata, Shigehiro, Oguma, Keiji, Ohyama, Tohru

“…The nontoxic–nonhemagglutinin (NTNHA) component, in both isolated form and the neurotoxin (NT)/NTNHA complexed form, was prepared protease-free from toxin…”
Get full text

Role of C-Terminal Region of HA-33 Component of Botulinum Toxin in Hemagglutination by Sagane, Yoshimasa, Kouguchi, Hirokazu, Watanabe, Toshihiro, Sunagawa, Hiroyuki, Inoue, Kaoru, Fujinaga, Yukako, Oguma, Keiji, Ohyama, Tohru

“…Using SDS–PAGE, we found that one subcomponent, hemagglutinin (HA-33), from the Clostridium botulinum progenitor toxin of type D strain 1873 and type C strain…”
Get full text

Indoor Airborne Mold Spores in Newly Built Dwellings by SAIJO, Yasuaki, SATA, Fumihiro, MIZUNO, Shintaro, YAMAGUCHI, Keiji, SUNAGAWA, Hiroyuki, KISHI, Reiko

“…Objective：To investigate the relationships between sick building syndrome and mold in newly-built dwellings. Methods：Symptoms of 61 residents in 18 dwellings…”
Get full text

Simple Method for Detection of Clostridium botulinum Type A to F Neurotoxin Genes by Ploymerase Chain Reaction by Takeshi, Kouichi, Fujinaga, Yukako, Inoue, Kaoru, Nakajima, Hiroshi, Oguma, Keiji, Ueno, Tetsuya, Sunagawa, Hiroyuki, Ohyama, Tohru

“…A polymerase chain reaction (PCR)-based method was established to detect each type of neurotoxin genes of Clostridium botulinum types A to F by employing the…”
Get full text

Indoor Airborne Mold Spores in Newly Built Dwellings by Saijo, Yasuaki, Sata, Fumihiro, Mizuno, Shintaro, Yamaguchi, Keiji, Sunagawa, Hiroyuki, Kishi, Reiko

“…Objective: To investigate the relationships between sick building syndrome and mold in newly-built dwellings. Methods: Symptoms of 61 residents in 18 dwellings…”
Get full text

Molecular Composition of the 16S Toxin Produced by a Clostridium botulinum Type D Strain, 1873 by Nakajima, Hiroshi, Inoue, Kaoru, Ikeda, Tetsuya, Fujinaga, Yukako, Sunagawa, Hiroyuki, Takeshi, Kouichi, Ohyama, Tohru, Watanabe, Toshihiro, Inoue, Katsuhiro, Oguma, Keiji

“…The 16S toxin was purified from a Clostridium botulinum type D strain 1873 (D-1873). Furthermore, the entire nucleotide sequences of the genes coding for the…”
Get full text

Characterization of Nontoxic-Nonhemagglutinin Component of the Two Types of Progenitor Toxin (M and L) Produced by Clostridium botulinum Type D CB-16 by Ohyama, Tohru, Watanabe, Toshihiro, Fujinaga, Yukako, Inoue, Kaoru, Sunagawa, Hiroyuki, Fujii, Nobuhiro, Inoue, Katsuhiro, Oguma, Keiji

“…A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide…”
Get full text

Molecular characterization of GroES and GroEL homologues from Clostridium botulinum by Sagane, Yoshimasa, Hasegawa, Kimiko, Mutoh, Shingo, Kouguchi, Hirokazu, Suzuki, Tomonori, Sunagawa, Hiroyuki, Nakagawa, Tomoyuki, Kamaguchi, Arihide, Okasaki, Shinn, Nakayama, Kenji, Watanabe, Toshihiro, Oguma, Keiji, Ohyama, Tohru

“…We report novel findings of significant amounts of 60- and 10-kDa proteins on SDS-PAGE in a culture supernatant of the Clostridium botulinum type D strain 4947…”
Get full text

Dichain structure of botulinum neurotoxin: identification of cleavage sites in types C, D, and F neurotoxin molecules by Sagane, Y, Watanabe, T, Kouguchi, H, Sunagawa, H, Inoue, K, Fujinaga, Y, Oguma, K, Ohyama, T

“…Botulinum neurotoxin (NT) is synthesized by Clostridium botulinum as about a 150-kDa single-chain polypeptide. Posttranslational modification by bacterial or…”
Get full text

Molecular composition of progenitor toxin produced by Clostridium botulinum type C strain 6813 by Watanabe, T, Sagane, Y, Kouguchi, H, Sunagawa, H, Inoue, K, Fujinaga, Y, Oguma, K, Ohyama, T

“…The molecular composition of the purified progenitor toxin produced by a Clostridium botulinum type C strain 6813 (C-6813) was analyzed. The strain produced…”
Get full text

Characterization of nicking of the nontoxic-nonhemagglutinin components of Clostridium botulinum types C and D progenitor toxin by Sagane, Y, Watanabe, T, Kouguchi, H, Sunagawa, H, Inoue, K, Fujinaga, Y, Oguma, K, Ohyama, T

“…Clostridium botulinum C and D strains produce two types of progenitor toxins, M and L. Previously we reported that a 130-kDa nontoxic-nonhemagglutinin (NTNHA)…”
Get full text

Direct Detection by PCR of Escherichia coli O157 and Enteropathogens in Patients with Bloody Diarrhea by Takeshi, Kouichi, Ikeda, Tetsuya, Kubo, Akiko, Fujinaga, Yukako, Makino, Souichi, Oguma, Keiji, Isogai, Emiko, Yoshida, Shin-ichi, Sunagawa, Hiroyuki, Ohyama, Tohru, Kimura, Hiroo

“…Direct detection of Escherichia coli O157 and foodborne pathogens associated with bloody diarrhea were achieved using polymerase chain reaction (PCR) after the…”
Get full text

Heat Resistance, Spore Germination, and Enterotoxigenicity of Clostridium perfringens by Ando, Yoshiaki, Tsuzuki, Toshibumi, Sunagawa, Hiroyuki, Oka, Shigemi

“…Heat resistance at 95C, heat activation at 75C, and germination response were determined for spores of 10 serotype strains of Clostridium perfringens type A,…”
Get full text

Protective Effects of Quercetin and Its Metabolites on H 2 O 2 -Induced Chromosomal Damage to WIL2-NS Cells by SAITO, Akiko, SUGISAWA, Ayako, UMEGAKI, Keizo, SUNAGAWA, Hiroyuki

Get full text

Direct and rapid detection by PCR of Erysipelothrix sp. DNAs prepared from bacterial strains and animal tissues by Takeshi, K, Makino, S, Ikeda, T, Takada, N, Nakashiro, A, Nakanishi, K, Oguma, K, Katoh, Y, Sunagawa, H, Ohyama, T

“…A PCR method for rapid screening of Erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers…”
Get full text

Incidence of Clostridium perfringens in Human Feces and Enterotoxin Production and Spore-Germination of the Isolates by SUNAGAWA, Hiroyuki, TAKESHI, Koichi, KAMEYAMA, Kunio, ANDO, Yoshiaki

“…Clostridium perfringens was detected in 322 (74.2%) fecal specimens heated at 75°C for 20min and in 76 (17.5%) fecal specimens heated at 100°C for 60min, out…”
Get full text

Influence of composition of subculture medium on the sporulation of Clostridium perfringens by Tsuzuki, T. (Hokkaido. Inst. of Public Health, Sapporo (Japan)), Sunagawa, H, Ando, Y

“…The effect of the composition of thioglycollate medium as a subculture medium on the subsequent sporulation of Clostridium perfringens NCTC 8238 in Duncan and…”
Get full text

Direct and Rapid Detection by PCR ofErysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues by Kouichi Takeshi, Souichi Makino, Tetsuya Ikeda, Noriko Takada, Atsushi Nakashiro, Kazunori Nakanishi, Keiji Oguma, Yoshinobu Katoh, Hiroyuki Sunagawa, Tohru Ohyama

“…Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
Get full text