Production and evaluation of leukocyte- and thrombocyte-rich fibrin membranes in birds

The aim of this study was the preparation and histological evaluation of Leukocyte- and Thrombocyte-Rich Fibrin (L-TRF) membranes obtained from the blood of four bird species. Forty adult healthy birds were divided into four groups of equal size: G1 - macaws, G2 - domestic chickens, G3 - parrots, G4...

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Bibliographic Details
Published in:	Acta veterinaria Hungarica (Budapest. 1983) Vol. 67; no. 2; p. 296
Main Authors:	Fernandes, Laís L, Rahal, Sheila C, Fabro, Alexandre T, Batah, Sabrina S, Hippólito, Alicia G, Bisca, Jacqueline M, Hirot, Inajara N, Teixeira, Carlos R
Format:	Journal Article
Language:	English
Published:	Hungary 01-06-2019
Subjects:	Animals Birds Blood Platelets - metabolism Chickens Fibrin - metabolism Leukocytes - metabolism Membranes - metabolism Parrots Platelet-Rich Fibrin - metabolism protocol Avian fibrin membrane L-TRF histology
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Summary:	The aim of this study was the preparation and histological evaluation of Leukocyte- and Thrombocyte-Rich Fibrin (L-TRF) membranes obtained from the blood of four bird species. Forty adult healthy birds were divided into four groups of equal size: G1 - macaws, G2 - domestic chickens, G3 - parrots, G4 - toco toucans. A total of 0.5 mL of blood was collected from each bird, put into a glass tube without anticoagulant and centrifuged at 3000 rpm for 10 min. L-TRF membranes produced after compression of the clot were processed for histological analysis. The ratio of thrombocytes/area was not significantly different among Groups G2, G3 and G4, but a significant difference was found between Groups G1 and G2 with the highest thrombocyte concentration/area in G1. The groups did not differ statistically in the number of leukocytes/area. The fibrin-to-cells ratio did not vary statistically among Groups G1, G2 and G3, but this ratio was significantly higher in Group G4 than in the other groups. The thrombocyte-to-leukocyte ratio was the highest in Group G1, but it did not differ among Groups G2, G3 and G4. In conclusion, the centrifugation protocol allowed the production of L-TRF membranes in the four bird species studied. Histologically, cell ratios were analogous in domestic chickens and parrots, and macaws had the highest ratio of thrombocytes.
ISSN:	0236-6290
DOI:	10.1556/004.2019.030