Effect of surface structure on cell growth prepared by the terminal immobilization method

Effect of surface structure on cell growth prepared by the terminal immobilization method

Substrate effects of surface morphology and chemical structure for cell cultures prepared by molecular terminal immobilization method were studied. When we focused attention on a phenyl group as a functional moiety, the cell growth on the surface prepared by the immobilization method showed a better...

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Bibliographic Details
Published in:Journal of applied polymer science Vol. 91; no. 5; pp. 3001 - 3008
Main Authors: Sakurai, Toshihiko, Mizokami, Hiroshi, Furukawa, Shinichi, Sakata, Masayo, Kunitake, Masashi, Hirayama, Chyuichi, Ihara, Hirotaka
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05-03-2004
Wiley
Subjects:
Applied sciences
biocompatibility
biological activity
cell growth
Exact sciences and technology
Physicochemistry of polymers
poly(amino acid)s
Polymer industry, paints, wood
Technology of polymers
terminal immobilization method
Cell structure
terminal immobilization method
Morphology
Immobilization
cell growth
Biocompatibility
Polymer
poly(amino acid)s
Biological activity
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Summary:Substrate effects of surface morphology and chemical structure for cell cultures prepared by molecular terminal immobilization method were studied. When we focused attention on a phenyl group as a functional moiety, the cell growth on the surface prepared by the immobilization method showed a better proliferation rate than that of a substrate prepared by the casting method. Further, from the results of mouse fibroblast L929 cell (L‐cell) growth on poly(amino acid)‐immobilized surfaces in Dulbecco's minimal essential medium containing 10% FBS, it was indicated that the amino group was more effective than the phenyl group, and that a slight difference of chemical structure had a substantial influence on cell growth. In addition, mouse bone marrow–derived Sp2/0‐Ag14 cell (Sp2/0 cell) culture in ASF‐104 serum‐free medium, poly(amino acid)‐immobilized substrates showed an almost equal proliferation rate to that in a serum‐containing medium. These results showed that effective cell growth can occur on immobilized surfaces, and that detection of a weak interaction depends on the functional groups. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3001–3008, 2004
Bibliography:ark:/67375/WNG-SGTNMCM7-5
ArticleID:APP13501
istex:BC3CA1EBD6AC9E483A13B04DEC3BD8F89EC775D9
ISSN:0021-8995
1097-4628
DOI:10.1002/app.13501