Evaluation of the Rapid Polymyxin NP test for detection of colistin susceptibility in Enterobacteriaceae isolated from Thai patients

Development and evaluations of the Rapid Polymyxin NP test for detection of colistin resistance in Enterobacteriaceae have been recently reported. In this study, we evaluated the performance of the test using a larger number of Enterobacteriaceae, and a larger proportion of isolates with a colistin...

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Bibliographic Details
Published in:	Diagnostic microbiology and infectious disease Vol. 92; no. 2; pp. 102 - 106
Main Authors:	Yainoy, Sakda, Hiranphan, Monchanok, Phuadraksa, Thanawat, Eiamphungporn, Warawan, Tiengrim, Surapee, Thamlikitkul, Visanu
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-10-2018
Subjects:	Anti-Bacterial Agents - pharmacology Antibiotics resistance Antimicrobial susceptibility testing Carbapenem-resistant Enterobacteriaceae Carbapenems - pharmacology Colistin Colistin - pharmacology Drug Resistance, Bacterial Enterobacteriaceae - drug effects Enterobacteriaceae Infections - microbiology Escherichia coli - drug effects Humans Klebsiella pneumoniae - drug effects Microbial Sensitivity Tests Polymyxins - pharmacology Rapid Polymyxin NP test Thailand Thailand Antimicrobial susceptibility testing Carbapenem-resistant Enterobacteriaceae Rapid Polymyxin NP test Colistin Antibiotics resistance
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Summary:	Development and evaluations of the Rapid Polymyxin NP test for detection of colistin resistance in Enterobacteriaceae have been recently reported. In this study, we evaluated the performance of the test using a larger number of Enterobacteriaceae, and a larger proportion of isolates with a colistin MIC close to the breakpoint. Out of 339 isolates, the Rapid Polymyxin NP test detected colistin resistance in 13 isolates of Escherichia coli, 213 isolates of Klebsiella pneumoniae, 9 isolates of Enterobacter aerogenes, and 10 isolates of the other Enterobacteriaceae species. Sensitivity and specificity of the test for detecting colistin resistance were 100% and 95.9%, respectively. Positive predictive value and negative predictive value were 98.3% and 100%, respectively. •Performance of the Rapid Polymyxin NP Test was assessed.•A total of 339 clinical isolates of Enterobacteriaceae were included.•Rapid Polymyxin NP Test is accurate to detect colistin-resistant Enterobacteriaceae.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2018.05.009