In Vivo Glycocalyx Expression by Staphylococcus aureus Phage Type 52/52A/80 in S. aureus Osteomyelitis

Osteomyelitic rat tibiae were examined by scanning electron microscopy for the extracellular glycocalyx of Staphylococcus aureus. S. aureus and fractured tibiae from normal rats were incubated together in vitro and examined similarly. Low magnification of endosteal Haversian portals from tibiae stud...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 156; no. 6; pp. 942 - 946
Main Authors:	Buxton, Thomas B., Horner, Jack, Hinton, Alma, Rissing, J. Peter
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-12-1987 University of Chicago Press
Subjects:	Animals Bacterial diseases Bacteriophage Typing Bacteriophages Biological and medical sciences Bone marrow Bones Collagens Culture Techniques Disease Models, Animal Erythrocytes Glycocalyx Glycoproteins - biosynthesis Human bacterial diseases Infectious diseases Male Medical sciences Microscopy, Electron, Scanning Original Articles Osteomyelitis Osteomyelitis - microbiology Polysaccharides - biosynthesis Polysaccharides, Bacterial - biosynthesis Rats Rats, Inbred Strains Staphylococcal Infections - microbiology Staphylococcal infections, streptococcal infections, pneumococcal infections Staphylococcus Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - metabolism Staphylococcus aureus - ultrastructure Tibia - microbiology Tibia - ultrastructure Tibial fractures Cell coat Rodentia Diseases of the osteoarticular system Infection Vertebrata Experimental disease Mammalia Osteitis Bacteriosis Bacteria Micrococcales Micrococcaceae Staphylococcal infection Staphylococcus aureus
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Summary:	Osteomyelitic rat tibiae were examined by scanning electron microscopy for the extracellular glycocalyx of Staphylococcus aureus. S. aureus and fractured tibiae from normal rats were incubated together in vitro and examined similarly. Low magnification of endosteal Haversian portals from tibiae studied in vivo and in vitro disclosed adherent S. aureus exuding glycocalyx that buried the organism in dense, coccoid-studded biofilms. The biofilm became progressively more dense over time in vitro and was exuberant at day 70 in vivo. S. aureus incubated in vitro without tibiae disclosed no glycocalyx. Bone chips studied in vitro disclosed staphylococci more commonly near the endosteal Haversian portals than on the intervening endosteal surfaces (mean ± SE, 280 ± 75 vs. 12 ± 3 per 2,500-µm2 field; P < .002 by Student's t test). Organisms within ostia were not counted, although they occluded 10%–40% of the ostium. Staphylococci were adherent to exposed woven material, perhaps collagen.
Bibliography:	Please address requests for reprints to Dr. J. Peter Rissing, Infectious Disease Section (111G), Veterans Administration Medical Center, Augusta, Georgia 30910. ark:/67375/HXZ-R8GS1CCB-P istex:BD0B5059C018DF12EE62596D1F36DA88D1B08F88 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1899 1537-6613
DOI:	10.1093/infdis/156.6.942