Search Results - "Hine, A V"

Expression of the ubiquitin-proteasome pathway and muscle loss in experimental cancer cachexia by KHAL, J, WYKE, S. M, RUSSELL, S. T, HINE, A. V, TISDALE, M. J

“…Muscle protein degradation is thought to play a major role in muscle atrophy in cancer cachexia. To investigate the importance of the ubiquitin-proteasome…”
Get full text

Dual affinity method for plasmid DNA purification in aqueous two-phase systems by Barbosa, H.S.C., Hine, A.V., Brocchini, S., Slater, N.K.H., Marcos, J.C.

“…The DNA binding fusion protein, LacI–His 6–GFP, together with the conjugate PEG–IDA–Cu(II) (10 kDa) was evaluated as a dual affinity system for the pUC19…”
Get full text

Affinity partitioning of plasmid DNA with a zinc finger protein by Barbosa, H., Hine, A.V., Brocchini, S., Slater, N.K.H., Marcos, J.C.

“…The affinity isolation of pre-purified plasmid DNA (pDNA) from model buffer solutions using native and poly(ethylene glycol) (PEG) derivatized zinc finger–GST…”
Get full text

Beyond the Natural Proteome: Nondegenerate Saturation Mutagenesis-Methodologies and Advantages by Ferreira Amaral, M M, Frigotto, L, Hine, A V

“…Beyond the natural proteome, high-throughput mutagenesis offers the protein engineer an opportunity to "tweak" the wild-type activity of a protein to create a…”
Get more information

Increased expression of proteasome subunits in skeletal muscle of cancer patients with weight loss by Khal, J., Hine, A.V., Fearon, K.C.H., Dejong, C.H.C., Tisdale, M.J.

“…Atrophy of skeletal muscle is common in patients with cancer and results in increased morbidity and mortality. In order to design effective therapy the…”
Get full text

A new randomization assay reveals unexpected elements of sequence bias in model ‘randomized’ gene libraries: implications for biopanning by Palfrey, D., Picardo, M., Hine, A.V.

“…Although techniques such as biopanning rely heavily upon the screening of randomized gene libraries, there is surprisingly little information available on the…”
Get full text

Removing the redundancy from randomised gene libraries by Hughes, Marcus D, Nagel, David A, Santos, Albert F, Sutherland, Andrew J, Hine, Anna V

“…Amino acid substitution plays a vital role in both the molecular engineering of proteins and analysis of structure-activity relationships. High-throughput…”
Get full text

Simplified preparation of human arterial sections for PCR analysis of Chlamydia pneumoniae and human DNA by Palfrey, D, Cook, P J, Smythe, J A, Lip, G Y, Hine, A V

“…AIMS: To investigate multiple techniques for the preparation of solid tissue for polymerase chain reaction (PCR) analysis, and to identify the most simple…”
Get full text

Protein-mediated isolation of plasmid DNA by a zinc finger-glutathione S-transferase affinity linker by Woodgate, John, Palfrey, David, Nagel, David A., Hine, Anna V., Slater, Nigel K. H.

“…The sequence‐specific affinity chromatographic isolation of plasmid DNA from crude lysates of E. coli DH5α fermentations is addressed. A zinc finger‐GST fusion…”
Get full text

Synthesis and expression of a gene encoding a 48-residue repeat in the Pseudomonas syringae ice nucleation protein by Hine, A V, Brown, T A

“…The aim of this work was to construct short analogues of the repetitive water-binding domain of the Pseudomonas syringae ice nucleation protein, InaZ…”
Get more information

A functional chimeric DNA primase: the Cys4 zinc-binding domain of bacteriophage T3 primase fused to the helicase of bacteriophage T7 by Hine, A V, Richardson, C C

“…Two colinear bacteriophage T7 gene 4 proteins provide helicase and primase functions in vivo. T7 primase differs from T7 helicase by an additional 63 residues…”
Get full text

The Cys4 zinc finger of bacteriophage T7 primase in sequence-specific single-stranded DNA recognition by Kusakabe, T, Hine, A V, Hyberts, S G, Richardson, C C

“…Bacteriophage T7 DNA primase recognizes 5′-GTC-3′ in single-stranded DNA. The primase contains a single Cys 4 zinc-binding motif that is essential for…”
Get full text

Solid-Phase PNA Synthesis and In Situ Scintillation Proximity Assay for the Detection of PNA−DNA Hybridization by McCairn, Mark C, Hughes, Marcus D, Hine, Anna V, Sutherland, Andrew J

Get full text

The Cys sub(4) zinc finger of bacteriophage T7 primase in sequence-specific single-stranded DNA recognition by Kusakabe, T, Hine, A V, Hyberts, S G, Richardson, C C

“…Bacteriophage T7 DNA primase recognizes 5'- GTC-3' in single-stranded DNA. The primase contains a single Cys sub(4) zinc-binding motif that is essential for…”
Get full text

LacI-mediated sequence-specific affinity purification of plasmid DNA for therapeutic applications by Darby, Richard A. J, Hine, Anna V

“…ABSTRACT Affinity purification of plasmid DNA is an attractive option for the biomanufacture of therapeutic plasmids, which are strictly controlled for levels…”
Get full text

A functional chimeric DNA primase: The Cys sub(4) zinc-binding domain of bacteriophage T3 primase fused to the helicase of bacteriophage T7 by Hine, A V, Richardson, C C

“…Two colinear bacteriophage T7 gene 4 proteins provide helicase and primase functions in vivo. T7 primase differs from T7 helicase by an additional 63 residues…”
Get full text

Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins by Hughes, Marcus D., Zhang, Zhan-Ren, Sutherland, Andrew J., Santos, Albert F., Hine, Anna V.

“…We have successfully linked protein library screening directly with the identification of active proteins, without the need for individual purification,…”
Get full text

Fluorescent microplate-based analysis of protein-DNA interactions. II: Immobilized DNA by Zhang, Zhan-Ren, Hughes, Marcus D, Morgan, Leonie J, Santos, Albert F, Hine, Anna V

“…A simple protein-DNA interaction analysis has been developed using both a high-affinity/high-specificity zinc finger protein and a low-specificity zinc finger…”
Get full text

Fluorescent microplate-based analysis of protein-DNA interactions. I: Immobilized protein by Zhang, Zhan-Ren, Palfrey, David, Nagel, David A, Lambert, Peter A, Jessop, Robert A, Santos, Albert F, Hine, Anna V

“…A simple protein-DNA interaction analysis has been developed using a high-affinity/high-specificity zinc finger protein. In essence, purified protein samples…”
Get full text

A Platform for Both Solid-Phase Peptide Nucleic Acid Oligomer Synthesis and Subsequent in Situ Detection and Quantification of Nucleic Add Sequences by McCairn, M C, Hughes, MD, Hine, A V, Sutherland, A J

Get full text