Detection of multiple viral sequences in the respiratory tract samples of suspected Middle East respiratory syndrome coronavirus patients in Jakarta, Indonesia 2015–2016

•Nine viral etiological agents were identified from 13 MERS-CoV patients with pneumonia in Indonesia from 2015- to 2016.•Patients with lower respiratory infections and travel history to Middle East should be screened for other respiratory viruses in addition to MERS-CoV.•This study confirmed that th...

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Bibliographic Details
Published in:	International journal of infectious diseases Vol. 86; pp. 102 - 107
Main Authors:	Setianingsih, Tri Yuli, Wiyatno, Ageng, Hartono, Teguh Sarry, Hindawati, Evi, Rosamarlina, Dewantari, Aghnianditya Kresno, Myint, Khin Saw, Lisdawati, Vivi, Safari, Dodi
Format:	Journal Article
Language:	English
Published:	Canada Elsevier Ltd 01-09-2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases Elsevier
Subjects:	Adult Aged Coronavirus - isolation & purification Coronavirus Infections - virology Female Hospitalization Humans Indonesia Male MERS-CoV Middle Aged Middle East PCR Pneumonia Respiratory System - virology RNA, Viral - chemistry Sequence Analysis, RNA Travel Viral Viruses - genetics Viruses - isolation & purification Middle East Indonesia Pneumonia MERS-CoV PCR Viral Indonesia
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Summary:	•Nine viral etiological agents were identified from 13 MERS-CoV patients with pneumonia in Indonesia from 2015- to 2016.•Patients with lower respiratory infections and travel history to Middle East should be screened for other respiratory viruses in addition to MERS-CoV.•This study confirmed that there was no MERS-CoV in the 13 suspected MERS-CoV patients with pneumonia. The identification and analysis of viral etiological agents from suspected Middle East respiratory syndrome coronavirus (MERS-CoV) cases admitted to Prof. Dr. Sulianti Saroso Infectious Disease Hospital (IDH) using molecular assays. Biological samples were collected from 13 hospitalized patients suspected of MERS-CoV infection in Prof. Dr. Sulianti Saroso IDH from July 2015 to December 2016. The majority of patients presented with pneumonia, with symptoms including fever (≥37.5 °C), labored breathing, and cough, and with a history of travel to the Middle East. Viral RNA was isolated and converted to cDNA, which was used as a template for the detection of 12 viral panels using conventional PCR and sequencing. Viral etiological agents detected in the patients were enterovirus D68, dengue virus type 3, rhinovirus C, human coronavirus 229E, herpes simplex virus type 1, influenza virus H1N1, influenza virus H3N2, human metapneumovirus, and rhinovirus A60. The sequences of nine viral agents under different taxa were detected in suspected MERS-CoV patients, including influenza virus, paramyxovirus, coronavirus, enterovirus, human metapneumovirus, and herpesvirus.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1201-9712 1878-3511 1878-3511
DOI:	10.1016/j.ijid.2019.06.022