Cloning of a murine IL‐11 receptor alpha‐chain; requirement for gp130 for high affinity binding and signal transduction

Cloning of a murine IL‐11 receptor alpha‐chain; requirement for gp130 for high affinity binding and signal transduction

An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structu...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal Vol. 13; no. 20; pp. 4765 - 4775
Main Authors: Hilton, D.J., Hilton, A.A., Raicevic, A., Rakar, S., Harrison‐Smith, M., Gough, N.M., Begley, C.G., Metcalf, D., Nicola, N.A., Willson, T.A.
Format: Journal Article
Language:English
Published: England 17-10-1994
Subjects:
3T3 Cells
Amino Acid Sequence
Animals
Antigens, CD
Base Sequence
Cell Division - genetics
Cell Line
Cloning, Molecular
Cytokine Receptor gp130
DNA, Complementary
Interleukin-11 Receptor alpha Subunit
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Membrane Glycoproteins - physiology
Mice
Molecular Sequence Data
Receptors, Interleukin - chemistry
Receptors, Interleukin - metabolism
Receptors, Interleukin - physiology
Receptors, Interleukin-11
RNA, Messenger - genetics
Sequence Homology, Amino Acid
Signal Transduction
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the alpha‐chain of the IL‐6 receptor and mRNA was expressed in the 3T3‐L1 pre‐adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor‐dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL‐11 (Kd approximately 10 nM). The capacity to bind IL‐11 with high affinity (Kd = 300‐800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL‐6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL‐11.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1994.tb06802.x