Search Results - "Higgs, H N"

Regulation of actin filament network formation through ARP2/3 complex: activation by a diverse array of proteins by Higgs, H N, Pollard, T D

“…Actin filament assembly and turnover drive many forms of cellular motility, particularly extension of the leading edge of locomoting cells and rocketing of…”
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Crystal Structure of Arp2/3 Complex by Robinson, Robert C., Turbedsky, Kirsi, Kaiser, Donald A., Marchand, Jean-Baptiste, Higgs, Henry N., Choe, Senyon, Pollard, Thomas D.

“…We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0…”
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Sequence of the Intron/Exon Junctions of the Coding Region of the Human Androgen Receptor Gene and Identification of a Point Mutation in a Family with Complete Androgen Insensitivity by Lubahn, Dennis B., Brown, Terry R., Simental, Jorge A., Higgs, Henry N., Migeon, Claude J., Wilson, Elizabeth M., French, Frank S.

“…Androgens act through a receptor protein (AR) to mediate sex differentiation and development of the male phenotype. We have isolated the eight exons in the…”
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Actin Cytoskeleton: Formins Lead the Way by Harris, E.S, Higgs, H.N

“…Formins are eukaryotic proteins that potently influence actin polymerization dynamics. Recent evidence strongly suggests that these proteins move processively…”
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The human androgen receptor: complementary deoxyribonucleic acid cloning, sequence analysis and gene expression in prostate by Lubahn, D B, Joseph, D R, Sar, M, Tan, J, Higgs, H N, Larson, R E, French, F S, Wilson, E M

“…Androgenic hormones mediate their effects on male sex differentiation and development through a high affinity receptor protein. We report here cloning of the…”
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Regulation of Actin Polymerization by Arp2/3 Complex and WASp/Scar Proteins by Higgs, Henry N., Pollard, Thomas D.

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Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex by Pollard, Thomas D, Marchand, Jean-Baptiste, Kaiser, Donald A, Higgs, Henry N

“…The Wiskott-Aldrich-syndrome protein (WASP) regulates polymerization of actin by the Arp2/3 complex. Here we show, using fluorescence anisotropy assays, that…”
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Scar, a WASp-Related Protein, Activates Nucleation of Actin Filaments by the Arp2/3 Complex by Machesky, Laura M., Mullins, R. Dyche, Higgs, Henry N., Kaiser, Donald A., Blanchoin, Laurent, May, Robin C., Hall, Margaret E., Pollard, Thomas D.

“…The Arp2/3 complex, a stable assembly of two actin-related proteins (Arp2 and Arp3) with five other subunits, caps the pointed end of actin filaments and…”
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Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins by Pollard, Thomas D, Blanchoin, Laurent, Amann, Kurt J, Higgs, Henry N, Marchand, Jean-Baptiste, Kaiser, Donald A

“…Most nucleated cells crawl about by extending a pseudopod that is driven by the polymerization of actin filaments in the cytoplasm behind the leading edge of…”
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Influence of the C Terminus of Wiskott-Aldrich Syndrome Protein (WASp) and the Arp2/3 Complex on Actin Polymerization by Higgs, Henry N, Blanchoin, Laurent, Pollard, Thomas D

“…The 70 C-terminal amino acids of Wiskott-Aldrich syndrome protein (WASp WA) activate the actin nucleation activity of the Arp2/3 complex. WASp WA binds both…”
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Actin Dynamics: Growth from Dendritic Branches by Nicholson-Dykstra, Susan, Higgs, Henry N., Harris, Elizabeth S.

“…The dendritic nucleation model was devised to explain the cycle of actin dynamics resulting in actin filament network assembly and disassembly in two contexts…”
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Interactions of Acanthamoeba Profilin with Actin and Nucleotides Bound to Actin by Vinson, Valda K, De La Cruz, Enrique M, Higgs, Henry N, Pollard, Thomas D

“…Three methods, fluorescence anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the same affinity, K d = 0.1 μM, for…”
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Identification of a phosphatidic acid-preferring phospholipase A1 from bovine brain and testis by Higgs, H N, Glomset, J A

“…Recent experiments in several laboratories have provided evidence that phosphatidic acid functions in cell signaling. However, the mechanisms that regulate…”
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The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes by May, Robin C., Hall, Margaret E., Higgs, Henry N., Pollard, Thomas D., Chakraborty, Trinad, Wehland, Juergen, Machesky, Laura M., Sechi, Antonio S.

“…Actin polymerisation is thought to drive the movement of eukaryotic cells and some intracellular pathogens such as Listeria monocytogenes. The Listeria surface…”
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The Verprolin-like Central (VC) Region of Wiskott-Aldrich Syndrome Protein Induces Arp2/3 Complex-dependent Actin Nucleation by Hüfner, Katharina, Higgs, Henry N., Pollard, Thomas D., Jacobi, Christoph, Aepfelbacher, Martin, Linder, Stefan

“…Wiskott-Aldrich Syndrome protein (WASp) and related proteins stimulate actin filament nucleation by Arp2/3 complex. The isolated C-terminal VCA domain of WASp…”
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Actin nucleation: Nucleation-promoting factors are not all equal by Higgs, Henry N

“…Arp2/3 complex plays a key role in regulated actin polymerization. A recent study has revealed marked differences in the ability of two nucleation-promoting…”
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Membrane Lipids Have Multiple Effects on Interfacial Catalysis by a Phosphatidic Acid-Preferring Phospholipase A1 from Bovine Testis by Lin, Qishan, Higgs, Henry N, Glomset, John A

“…We previously purified a cytosolic phospholipase A1 that could catalyze the preferential hydrolysis of phosphatidic acid in mixed-micelle assays. Here we…”
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Tools for ‘The Sceptical Chymist’: measuring macromolecular interaction kinetics in live cells by TIR–FRAP by HIGGS, HENRY N

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Cdc42-induced actin filaments are protected from capping protein by Huang, Minzhou, Yang, Changsong, Schafer, Dorothy A., Cooper, John A., Higgs, Henry N., Zigmond, Sally H.

“…Each actin filament has a pointed and a barbed end, however, filament elongation occurs primarily at the barbed end. Capping proteins, by binding to the barbed…”
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Formin proteins: a domain-based approach by Higgs, Henry N.

“…Formin proteins are potent regulators of actin dynamics. Most eukaryotes have multiple formin isoforms, suggesting diverse cellular roles. Formins are modular…”
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