Search Results - "Hickman, Alison B"

Structural basis of seamless excision and specific targeting by piggyBac transposase by Chen, Qiujia, Luo, Wentian, Veach, Ruth Ann, Hickman, Alison B., Wilson, Matthew H., Dyda, Fred

“…The piggyBac DNA transposon is used widely in genome engineering applications. Unlike other transposons, its excision site can be precisely repaired without…”
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Breaking and joining single-stranded DNA: the HUH endonuclease superfamily by Chandler, Michael, de la Cruz, Fernando, Dyda, Fred, Hickman, Alison B., Moncalian, Gabriel, Ton-Hoang, Bao

“…Key Points HUH endonucleases contain the characteristic HUH motif (in which U represents a hydrophobic residue) and a Y motif, containing either one or two Tyr…”
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Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding by Lannes, Laurie, Furman, Christopher M., Hickman, Alison B., Dyda, Fred

“…The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation,…”
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Helraiser intermediates provide insight into the mechanism of eukaryotic replicative transposition by Grabundzija, Ivana, Hickman, Alison B., Dyda, Fred

“…Helitrons are eukaryotic DNA transposons that have profoundly affected genome variability via capture and mobilization of host genomic sequences. Defining…”
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Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa by He, Susu, Taher, Noor M, Simard, Adam R, Hvorecny, Kelli L, Ragusa, Michael J, Bahl, Christopher D, Hickman, Alison B, Dyda, Fred, Madden, Dean R

“…The opportunistic pathogen Pseudomonas aeruginosa infects the airways of people with cystic fibrosis (CF) and produces a virulence factor Cif that is…”
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Mechanisms of DNA Transposition by Hickman, Alison B, Dyda, Fred

“…DNA transposases use a limited repertoire of structurally and mechanistically distinct nuclease domains to catalyze the DNA strand breaking and rejoining…”
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Mechanisms of Evolution in High-Consequence Drug Resistance Plasmids by He, Susu, Chandler, Michael, Varani, Alessandro M, Hickman, Alison B, Dekker, John P, Dyda, Fred

“…The dissemination of resistance among bacteria has been facilitated by the fact that resistance genes are usually located on a diverse and evolving set of…”
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Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas by Hickman, Alison B, Kailasan, Shweta, Genzor, Pavol, Haase, Astrid D, Dyda, Fred

“…Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates…”
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Structural Basis of hAT Transposon End Recognition by Hermes, an Octameric DNA Transposase from Musca domestica by Hickman, Alison B., Ewis, Hosam E., Li, Xianghong, Knapp, Joshua A., Laver, Thomas, Doss, Anna-Louise, Tolun, Gökhan, Steven, Alasdair C., Grishaev, Alexander, Bax, Ad, Atkinson, Peter W., Craig, Nancy L., Dyda, Fred

“…Hermes is a member of the hAT transposon superfamily that has active representatives, including McClintock’s archetypal Ac mobile genetic element, in many…”
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Polymerization and nucleic acid-binding properties of human L1 ORF1 protein by Callahan, Kathryn E., Hickman, Alison B., Jones, Charles E., Ghirlando, Rodolfo, Furano, Anthony V.

“…The L1 (LINE 1) retrotransposable element encodes two proteins, ORF1p and ORF2p. ORF2p is the L1 replicase, but the role of ORF1p is unknown. Mouse ORF1p, a…”
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DNA Transposition at Work by Hickman, Alison B, Dyda, Fred

“…DNA transposons are defined segments of DNA that are able to move from one genomic location to another. Movement is facilitated by one or more proteins, called…”
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A retrotransposon for site-specific gene transfer by Dyda, Fred, Hickman, Alison B

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The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications by Hickman, Alison B, Dyda, Fred

“…Many archaea and bacteria have an adaptive immune system known as CRISPR which allows them to recognize and destroy foreign nucleic acid that they have…”
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A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1 by Snesrud, Erik, He, Susu, Chandler, Michael, Dekker, John P, Hickman, Alison B, McGann, Patrick, Dyda, Fred

“…Analysis of mcr-1-containing sequences identified a common ∼2,607-bp DNA segment that in many cases is flanked on one or both ends by ISApl1 We present…”
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Three New Structures of the Core Domain of HIV-1 Integrase: An Active Site that Binds Magnesium by Goldgur, Yehuda, Dyda, Fred, Hickman, Alison B., Jenkins, Timothy M., Craigie, Robert, Davies, David R.

“…HIV-1 integrase is an essential enzyme in the life cycle of the virus, responsible for catalyzing the insertion of the viral genome into the host cell…”
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Structures of ISCth4 transpososomes reveal the role of asymmetry in copy‐out/paste‐in DNA transposition by Kosek, Dalibor, Hickman, Alison B, Ghirlando, Rodolfo, He, Susu, Dyda, Fred

“…Copy‐out/paste‐in transposition is a major bacterial DNA mobility pathway. It contributes significantly to the emergence of antibiotic resistance, often by…”
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The large bat Helitron DNA transposase forms a compact monomeric assembly that buries and protects its covalently bound 5′-transposon end by Kosek, Dalibor, Grabundzija, Ivana, Lei, Haotian, Bilic, Ilija, Wang, Huaibin, Jin, Yukun, Peaslee, Graham F., Hickman, Alison B., Dyda, Fred

“…Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to genome variability and evolution, in part because of their…”
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Crystal Structure of the Catalytic Domain of HIV-1 Integrase: Similarity to Other Polynucleotidyl Transferases by Dyda, Fred, Hickman, Alison B., Jenkins, Timothy M., Engelman, Alan, Craigie, Robert, Davies, David R.

“…HIV integrase is the enzyme responsible for inserting the viral DNA into the host chromosome; it is essential for HIV replication. The crystal structure of the…”
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Toxic antiphage defense proteins inhibited by intragenic antitoxin proteins by Zhong, Aoshu, Jiang, Xiaofang, Hickman, Alison B, Klier, Katherine, Teodoro, Gabriella I C, Dyda, Fred, Laub, Michael T, Storz, Gisela

“…Recombination-promoting nuclease (Rpn) proteins are broadly distributed across bacterial phyla, yet their functions remain unclear. Here, we report that these…”
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Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells by Luo, Wentian, Hickman, Alison B, Genzor, Pavol, Ghirlando, Rodolfo, Furman, Christopher M, Menshikh, Anna, Haase, Astrid, Dyda, Fred, Wilson, Matthew H

“…DNA transposon systems are widely used in mammalian cells for genetic modification experiments, but their regulation remains poorly understood. We used…”
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