Search Results - "Heymann, Michael"

Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations by Litschel, Thomas, Ramm, Beatrice, Maas, Roel, Heymann, Michael, Schwille, Petra

“…The bacterial Min protein system was encapsulated in giant unilamellar vesicles (GUVs). Using confocal fluorescence microscopy, we identified several distinct…”
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Testing Turing's theory of morphogenesis in chemical cells by Tompkins, Nathan, Li, Ning, Girabawe, Camille, Heymann, Michael, Ermentrout, G. Bard, Epstein, Irving R., Fraden, Seth

“…Alan Turing, in "The Chemical Basis of Morphogenesis" [Turing AM (1952) Philos Trans R Soc Lond 237(641):37—72], described how, in circular arrays of identical…”
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Ultracompact 3D microfluidics for time-resolved structural biology by Knoška, Juraj, Adriano, Luigi, Awel, Salah, Beyerlein, Kenneth R., Yefanov, Oleksandr, Oberthuer, Dominik, Peña Murillo, Gisel E., Roth, Nils, Sarrou, Iosifina, Villanueva-Perez, Pablo, Wiedorn, Max O., Wilde, Fabian, Bajt, Saša, Chapman, Henry N., Heymann, Michael

“…To advance microfluidic integration, we present the use of two-photon additive manufacturing to fold 2D channel layouts into compact free-form 3D fluidic…”
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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein by Tenboer, Jason, Basu, Shibom, Zatsepin, Nadia, Pande, Kanupriya, Milathianaki, Despina, Frank, Matthias, Hunter, Mark, Boutet, Sébastien, Williams, Garth J., Koglin, Jason E., Oberthuer, Dominik, Heymann, Michael, Kupitz, Christopher, Conrad, Chelsie, Coe, Jesse, Roy-Chowdhury, Shatabdi, Weierstall, Uwe, James, Daniel, Wang, Dingjie, Grant, Thomas, Barty, Anton, Yefanov, Oleksandr, Scales, Jennifer, Gati, Cornelius, Seuring, Carolin, Srajer, Vukica, Henning, Robert, Schwander, Peter, Fromme, Raimund, Ourmazd, Abbas, Moffat, Keith, Van Thor, Jasper J., Spence, John C. H., Fromme, Petra, Chapman, Henry N., Schmidt, Marius

“…Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of…”
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Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks by Jia, Haiyang, Heymann, Michael, Bernhard, Frank, Schwille, Petra, Kai, Lei

“…•Bottom-up construction of minimal cells.•Advances in cell free protein synthesis in synthetic biology.•Microfluidic high-throughput methods. The construction…”
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A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a by Katzmeier, Florian, Aufinger, Lukas, Dupin, Aurore, Quintero, Jorge, Lenz, Matthias, Bauer, Ludwig, Klumpe, Sven, Sherpa, Dawafuti, Dürr, Benedikt, Honemann, Maximilian, Styazhkin, Igor, Simmel, Friedrich C, Heymann, Michael

“…Point-of-care testing (POCT) in low-resource settings requires tools that can operate independently of typical laboratory infrastructure. Due to its favorable…”
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FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis by Ramirez-Diaz, Diego A., Merino-Salomón, Adrián, Meyer, Fabian, Heymann, Michael, Rivas, Germán, Bramkamp, Marc, Schwille, Petra

“…FtsZ is a key component in bacterial cell division, being the primary protein of the presumably contractile Z ring. In vivo and in vitro, it shows two…”
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Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles by Litschel, Thomas, Ganzinger, Kristina A, Movinkel, Torgeir, Heymann, Michael, Robinson, Tom, Mutschler, Hannes, Schwille, Petra

“…Early protocells are commonly assumed to consist of an amphiphilic membrane enclosing an RNA-based self-replicating genetic system and a primitive metabolism…”
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Shaping Giant Membrane Vesicles in 3D‐Printed Protein Hydrogel Cages by Jia, Haiyang, Litschel, Thomas, Heymann, Michael, Eto, Hiromune, Franquelim, Henri G., Schwille, Petra

“…Giant unilamellar phospholipid vesicles are attractive starting points for constructing minimal living cells from the bottom‐up. Their membranes are compatible…”
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The MinDE system is a generic spatial cue for membrane protein distribution in vitro by Ramm, Beatrice, Glock, Philipp, Mücksch, Jonas, Blumhardt, Philipp, García-Soriano, Daniela A., Heymann, Michael, Schwille, Petra

“…The E. coli MinCDE system has become a paradigmatic reaction–diffusion system in biology. The membrane-bound ATPase MinD and ATPase-activating protein MinE…”
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Cell-free expression of RNA encoded genes using MS2 replicase by Weise, Laura I, Heymann, Michael, Mayr, Viktoria, Mutschler, Hannes

“…Abstract RNA replicases catalyse transcription and replication of viral RNA genomes. Of particular interest for in vitro studies are phage replicases due to…”
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Rapid prototyping of cyclic olefin copolymer (COC) microfluidic devices by Aghvami, S. Ali, Opathalage, Achini, Zhang, Z.K., Ludwig, Markus, Heymann, Michael, Norton, Michael, Wilkins, Niya, Fraden, Seth

“…•Low cost prototyping of thermoplastic microfluidics.•Movies illustrate all process steps.•Surface treatments render channels hydrophilic, fluorophilic or…”
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Membrane association and remodeling by intraflagellar transport protein IFT172 by Wang, Qianmin, Taschner, Michael, Ganzinger, Kristina A., Kelley, Charlotte, Villasenor, Alethia, Heymann, Michael, Schwille, Petra, Lorentzen, Esben, Mizuno, Naoko

“…The cilium is an organelle used for motility and cellular signaling. Intraflagellar transport (IFT) is a process to move ciliary building blocks and signaling…”
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Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments by Yenupuri, Tej Varma, Rafie-Zinedine, Safi, Worbs, Lena, Heymann, Michael, Schulz, Joachim, Bielecki, Johan, Maia, Filipe R. N. C.

“…Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential…”
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3D printed devices and infrastructure for liquid sample delivery at the European XFEL by Vakili, Mohammad, Bielecki, Johan, Knoška, Juraj, Otte, Florian, Han, Huijong, Kloos, Marco, Schubert, Robin, Delmas, Elisa, Mills, Grant, de Wijn, Raphael, Letrun, Romain, Dold, Simon, Bean, Richard, Round, Adam, Kim, Yoonhee, Lima, Frederico A., Dörner, Katerina, Valerio, Joana, Heymann, Michael, Mancuso, Adrian P., Schulz, Joachim

“…The Sample Environment and Characterization (SEC) group of the European X‐ray Free‐Electron Laser (EuXFEL) develops sample delivery systems for the various…”
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Development of a flat jet delivery system for soft X‐ray spectroscopy at MAX IV by Gallo, Tamires, Adriano, Luigi, Heymann, Michael, Wrona, Agnieszka, Walsh, Noelle, Öhrwall, Gunnar, Callefo, Flavia, Skruszewicz, Slawomir, Namboodiri, Mahesh, Marinho, Ricardo, Schulz, Joachim, Valerio, Joana

“…One of the most challenging aspects of X‐ray research is the delivery of liquid sample flows into the soft X‐ray beam. Currently, cylindrical microjets are the…”
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Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack by Rafie-Zinedine, Safi, Varma Yenupuri, Tej, Worbs, Lena, Maia, Filipe R. N. C., Heymann, Michael, Schulz, Joachim, Bielecki, Johan

“…This work investigates the performance of the electrospray aerosol generator at the European X‐ray Free Electron Laser (EuXFEL). This generator is, together…”
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Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System by Hürtgen, Daniel, Mascarenhas, Judita, Heymann, Michael, Murray, Seán M., Schwille, Petra, Sourjik, Victor

“…A biomimetic system capable of replication and segregation of genetic material constitutes an essential component for the future design of a minimal synthetic…”
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Lipidic cubic phase serial millisecond crystallography using synchrotron radiation by Nogly, Przemyslaw, James, Daniel, Wang, Dingjie, White, Thomas A, Zatsepin, Nadia, Shilova, Anastasya, Nelson, Garrett, Liu, Haiguang, Johansson, Linda, Heymann, Michael, Jaeger, Kathrin, Metz, Markus, Wickstrand, Cecilia, Wu, Wenting, Båth, Petra, Berntsen, Peter, Oberthuer, Dominik, Panneels, Valerie, Cherezov, Vadim, Chapman, Henry, Schertler, Gebhard, Neutze, Richard, Spence, John, Moraes, Isabel, Burghammer, Manfred, Standfuss, Joerg, Weierstall, Uwe

“…Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly…”
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Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography by Gicquel, Yannig, Schubert, Robin, Kapis, Svetlana, Bourenkov, Gleb, Schneider, Thomas, Perbandt, Markus, Betzel, Christian, Chapman, Henry N, Heymann, Michael

“…This protocol describes fabricating microfluidic devices with low X-ray background optimized for goniometer based fixed target serial crystallography. The…”
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