The Significance of Hydrogen Sulfide for Arabidopsis Seed Germination

Hydrogen sulfide (H2S) recently emerged as an important gaseous signaling molecule in plants. In this study, we investigated the possible functions of H2S in regulating Arabidopsis seed germination. NaHS treatments delayed seed germination in a dose-dependent manner and were ineffective in releasing...

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Bibliographic Details
Published in:	Frontiers in plant science Vol. 7; p. 930
Main Authors:	Baudouin, Emmanuel, Poilevey, Aurélie, Hewage, Nishodi Indiketi, Cochet, Françoise, Puyaubert, Juliette, Bailly, Christophe
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers 27-06-2016 Frontiers Media S.A
Subjects:	Development Biology Life Sciences Plant Science seed dormancy seed germination cysteine desulfhydrase hydrogen sulfide Arabidopsis
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Summary:	Hydrogen sulfide (H2S) recently emerged as an important gaseous signaling molecule in plants. In this study, we investigated the possible functions of H2S in regulating Arabidopsis seed germination. NaHS treatments delayed seed germination in a dose-dependent manner and were ineffective in releasing seed dormancy. Interestingly, endogenous H2S content was enhanced in germinating seeds. This increase was correlated with higher activity of three enzymes (L-cysteine desulfhydrase, D-cysteine desulfhydrase, and β-cyanoalanine synthase) known as sources of H2S in plants. The H2S scavenger hypotaurine and the D/L cysteine desulfhydrase inhibitor propargylglycine significantly delayed seed germination. We analyzed the germinative capacity of des1 seeds mutated in Arabidopsis cytosolic L-cysteine desulfhydrase. Although the mutant seeds do not exhibit germination-evoked H2S formation, they retained similar germination capacity as the wild-type seeds. In addition, des1 seeds responded similarly to temperature and were as sensitive to ABA as wild type seeds. Taken together, these data suggest that, although its metabolism is stimulated upon seed imbibition, H2S plays, if any, a marginal role in regulating Arabidopsis seed germination under standard conditions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC4921499 Reviewed by: Vasileios Fotopoulos, Cyprus University of Technology, Cyprus; Alma Balestrazzi, University of Pavia, Italy This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science Edited by: Irene Murgia, Università degli Studi di Milano, Italy
ISSN:	1664-462X 1664-462X
DOI:	10.3389/fpls.2016.00930