Search Results - "Hess, T"

Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes by Hess, Gaelen T., Tycko, Josh, Yao, David, Bassik, Michael C.

“…The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput…”
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SLC19A1 Is an Importer of the Immunotransmitter cGAMP by Ritchie, Christopher, Cordova, Anthony F., Hess, Gaelen T., Bassik, Michael C., Li, Lingyin

“…2′3′-cyclic-GMP-AMP (cGAMP) is a second messenger that activates the antiviral stimulator of interferon genes (STING) pathway. We recently identified a novel…”
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Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions by Han, Kyuho, Jeng, Edwin E, Hess, Gaelen T, Morgens, David W, Li, Amy, Bassik, Michael C

“…An improved strategy for large-scale combinatorial CRISPR screening enables the identification of synergistic drug targets for cancer. Identification of…”
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Precisely and accurately localizing single emitters in fluorescence microscopy by Deschout, Hendrik, Zanacchi, Francesca Cella, Mlodzianoski, Michael, Diaspro, Alberto, Bewersdorf, Joerg, Hess, Samuel T, Braeckmans, Kevin

“…This first of two review articles provides an overview and practical introduction to the precise and accurate localization of single emitters for…”
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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells by Hess, Gaelen T, Frésard, Laure, Han, Kyuho, Lee, Cameron H, Li, Amy, Cimprich, Karlene A, Montgomery, Stephen B, Bassik, Michael C

“…Recruiting a hyperactive cytidine deaminase via the guide RNA to dCas9 allows for the introduction of diverse point mutations at the CRISPR target locus to…”
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LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of cGAMP by Lahey, Lauren J., Mardjuki, Rachel E., Wen, Xianlan, Hess, Gaelen T., Ritchie, Christopher, Carozza, Jacqueline A., Böhnert, Volker, Maduke, Merritt, Bassik, Michael C., Li, Lingyin

“…Extracellular 2′3′-cyclic-GMP-AMP (cGAMP) is an immunotransmitter exported by diseased cells and imported into host cells to activate the innate immune STING…”
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Static and Dynamic DNA Loops form AP-1-Bound Activation Hubs during Macrophage Development by Phanstiel, Douglas H., Van Bortle, Kevin, Spacek, Damek, Hess, Gaelen T., Shamim, Muhammad Saad, Machol, Ido, Love, Michael I., Aiden, Erez Lieberman, Bassik, Michael C., Snyder, Michael P.

“…The three-dimensional arrangement of the human genome comprises a complex network of structural and regulatory chromatin loops important for coordinating…”
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Accelerated calibrationless parallel transmit mapping using joint transmit and receive low‐rank tensor completion by Hess, Aaron T., Dragonu, Iulius, Chiew, Mark

“…Purpose To evaluate an algorithm for calibrationless parallel imaging to reconstruct undersampled parallel transmit field maps for the body and brain. Methods…”
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Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy by Hess, Samuel T., Girirajan, Thanu P.K., Mason, Michael D.

“…Biological structures span many orders of magnitude in size, but far-field visible light microscopy suffers from limited resolution. A new method for…”
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Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens by Morgens, David W., Wainberg, Michael, Boyle, Evan A., Ursu, Oana, Araya, Carlos L., Tsui, C. Kimberly, Haney, Michael S., Hess, Gaelen T., Han, Kyuho, Jeng, Edwin E., Li, Amy, Snyder, Michael P., Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.

“…CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function, but can be confounded by nuclease-induced toxicity at both on- and…”
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Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations by Mlodzianoski, Michael J, Curthoys, Nikki M, Gunewardene, Mudalige S, Carter, Sean, Hess, Samuel T

“…Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species…”
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Volumetric navigators for prospective motion correction and selective reacquisition in neuroanatomical MRI by Tisdall, M. Dylan, Hess, Aaron T., Reuter, Martin, Meintjes, Ernesta M., Fischl, Bruce, van der Kouwe, André J. W.

“…We introduce a novel method of prospectively compensating for subject motion in neuroanatomical imaging. Short three‐dimensional echo‐planar imaging volumetric…”
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Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7 by Wisnovsky, Simon, Möckl, Leonhard, Malaker, Stacy A., Pedram, Kayvon, Hess, Gaelen T., Riley, Nicholas M., Gray, Melissa A., Smith, Benjamin A. H., Bassik, Michael C., Moerner, W. E., Bertozzi, Carolyn R.

“…Glyco-immune checkpoint receptors, molecules that inhibit immune cell activity following binding to glycosylated cell-surface antigens, are emerging as…”
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Regulated Protein Denitrosylation by Cytosolic and Mitochondrial Thioredoxins by Benhar, Moran, Forrester, Michael T, Hess, Douglas T, Stamler, Jonathan S

“…Nitric oxide acts substantially in cellular signal transduction through stimulus-coupled S-nitrosylation of cysteine residues. The mechanisms that might…”
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Assessing future drought risks and wheat yield losses in England by Clarke, D, Hess, T M, Haro-Monteagudo, D, Semenov, M.A., Knox, J W

“…•Estimates of yield losses due to drought risk are sensitive to the assessment method•Drought severity indicators (DSI) likely under-estimate future average…”
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Implementing precision irrigation in a humid climate – Recent experiences and on-going challenges by Daccache, A., Knox, J.W., Weatherhead, E.K., Daneshkhah, A., Hess, T.M.

“…•Differences between conventional and precision irrigation investigated.•Water balance modelling used to assess potential water saving.•Challenges in deriving…”
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Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension by Webb, Watt W, Baumgart, Tobias, Hess, Samuel T

“…Lipid bilayer membranes-ubiquitous in biological systems and closely associated with cell function-exhibit rich shape-transition behaviour, including bud…”
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Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements by Tycko, Josh, Wainberg, Michael, Marinov, Georgi K., Ursu, Oana, Hess, Gaelen T., Ego, Braeden K., Aradhana, Li, Amy, Truong, Alisa, Trevino, Alexandro E., Spees, Kaitlyn, Yao, David, Kaplow, Irene M., Greenside, Peyton G., Morgens, David W., Phanstiel, Douglas H., Snyder, Michael P., Bintu, Lacramioara, Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.

“…Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these…”
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Site-specific analysis of protein S-acylation by resin-assisted capture[S] by Forrester, Michael T., Hess, Douglas T., Thompson, J. Will, Hultman, Rainbo, Moseley, M. Arthur, Stamler, Jonathan S., Casey, Patrick J.

“…Protein S-acylation is a major posttranslational modification whereby a cysteine thiol is converted to a thioester. A prototype is S-palmitoylation (fatty…”
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Process-Structure-Property Relationship Development in Large-Format Additive Manufacturing: Fiber Alignment and Ultimate Tensile Strength by Slattery, Lucinda K, McClelland, Zackery B, Hess, Samuel T

“…Parts made through additive manufacturing (AM) often exhibit mechanical anisotropy due to the time-based deposition of material and processing parameters. In…”
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