Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures

Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures

The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell’s homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 91; no. 24; pp. 15967 - 15973
Main Authors: Fernández, Roberto, Garate, Jone, Tolentino-Cortez, Tarson, Herraiz, Ainara, Lombardero, Laura, Ducrocq, Fabien, Rodríguez-Puertas, Rafael, Trifilieff, Pierre, Astigarraga, Egoitz, Barreda-Gómez, Gabriel, Fernández, José A
Format: Journal Article
Language:English
Published: United States American Chemical Society 17-12-2019
Subjects:
Analytical chemistry
Animal genetics
Biomarkers
Chemistry
Fingerprints
Genetics
Homeostasis
Immunohistochemistry
Life Sciences
Lipid composition
Lipids
Mass spectrometry
Mass spectroscopy
Methodology
Nucleus accumbens
Scientific imaging
Spectroscopy
Tissues
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Summary:The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell’s homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b04529