Search Results - "Herr, Amy E."

Protein immobilization techniques for microfluidic assays by Kim, Dohyun, Herr, Amy E.

“…Microfluidic systems have shown unequivocal performance improvements over conventional bench-top assays across a range of performance metrics. For example,…”
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DropBlot: single-cell western blotting of chemically fixed cancer cells by Liu, Yang, Herr, Amy E.

“…Archived patient-derived tissue specimens play a central role in understanding disease and developing therapies. To address specificity and sensitivity…”
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Microfluidic Western blotting by Hughes, Alex J., Herr, Amy E.

“…Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a…”
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In Situ Single‐Cell Western Blot on Adherent Cell Culture by Zhang, Yizhe, Naguro, Isao, Herr, Amy E.

“…Integrating 2D culture of adherent mammalian cells with single‐cell western blotting (in situ scWB) uses microfluidic design to eliminate the requirement for…”
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Joule Heating-Induced Dispersion in Open Microfluidic Electrophoretic Cytometry by Vlassakis, Julea, Herr, Amy E

“…While protein electrophoresis conducted in capillaries and microchannels offers high-resolution separations, such formats can be cumbersome to parallelize for…”
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Profiling protein expression in circulating tumour cells using microfluidic western blotting by Sinkala, Elly, Sollier-Christen, Elodie, Renier, Corinne, Rosàs-Canyelles, Elisabet, Che, James, Heirich, Kyra, Duncombe, Todd A., Vlassakis, Julea, Yamauchi, Kevin A., Huang, Haiyan, Jeffrey, Stefanie S., Herr, Amy E.

“…Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of…”
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Separation-encoded microparticles for single-cell western blotting by Gumuscu, Burcu, Herr, Amy E

“…Direct measurement of proteins from single cells has been realized at the microscale using microfluidic channels, capillaries, and semi-enclosed microwell…”
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In Situ Measurement of Thermodynamic Partitioning in Open Hydrogels by Su, Alison, Smith, Benjamin E, Herr, Amy E

“…Thermodynamic partitioning dictates solute loading and release from a hydrogel. Design of drug delivery vehicles, cell and tissue matrices, and immunoassay…”
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Automated microfluidic protein immunoblotting by Herr, Amy E, He, Mei

“…This protocol describes regional photopatterning of polyacrylamide gels in glass microfluidic devices as a platform for seamless integration of multiple assay…”
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Ferguson analysis of protein electromigration during single-cell electrophoresis in an open microfluidic device by Tan, Kristine Y, Herr, Amy E

“…In an open microfluidic device, we investigate protein polyacrylamide gel electrophoresis (PAGE) separation performance on single-cell lysate. Single-cell…”
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Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices by Pan, Qiong, Yamauchi, Kevin A, Herr, Amy E

“…New tools for measuring protein expression in individual cells complement single-cell genomics and transcriptomics. To characterize a population of individual…”
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3D projection electrophoresis for single-cell immunoblotting by Grist, Samantha M., Mourdoukoutas, Andoni P., Herr, Amy E.

“…Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce…”
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Quantitative UV-C dose validation with photochromic indicators for informed N95 emergency decontamination by Su, Alison, Grist, Samantha M, Geldert, Alisha, Gopal, Anjali, Herr, Amy E

“…With COVID-19 N95 shortages, frontline medical personnel are forced to reuse this disposable-but sophisticated-multilayer respirator. Widely used to…”
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Ultrashort Separation Length Homogeneous Electrophoretic Immunoassays Using On-Chip Discontinuous Polyacrylamide Gels by Hou, Chenlu, Herr, Amy E

“…To realize efficient homogeneous electrophoretic immunoassays, we introduce discontinuous polyacrylamide gels that enable quantitative assay completion in…”
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Microfluidic immunoassays as rapid saliva-based clinical diagnostics by Herr, Amy E, Hatch, Anson V, Throckmorton, Daniel J, Tran, Huu M, Brennan, James S, Giannobile, William V, Singh, Anup K

“…At present, point-of-care (POC) diagnostics typically provide a binary indication of health status (e.g., home pregnancy test strip). Before anticipatory use…”
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Measuring expression heterogeneity of single-cell cytoskeletal protein complexes by Vlassakis, Julea, Hansen, Louise L., Higuchi-Sanabria, Ryo, Zhou, Yun, Tsui, C. Kimberly, Dillin, Andrew, Huang, Haiyan, Herr, Amy E.

“…Multimeric cytoskeletal protein complexes orchestrate normal cellular function. However, protein-complex distributions in stressed, heterogeneous cell…”
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Single-cell immunoblotting resolves estrogen receptor-α isoforms in breast cancer by Kim, John J., Liang, Wenchuan, Kang, Chi-Chih, Pegram, Mark D., Herr, Amy E.

“…An array of isoforms of the nuclear estrogen receptor alpha (ER-α) protein contribute to heterogeneous response in breast cancer (BCa); yet, a single-cell…”
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Microfluidic integration for automated targeted proteomic assays by Hughes, Alex J, Lin, Robert K. C, Peehl, Donna M, Herr, Amy E

“…A dearth of protein isoform-based clinical diagnostics currently hinders advances in personalized medicine. A well-organized protein biomarker validation…”
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Electrophoretic cytometry of adherent cells by Su, Elaine J, Herr, Amy E

“…Cell-matrix and cell-cell interactions influence intracellular signalling and play an important role in physiologic and pathologic processes. Detachment of…”
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Mapping of UV-C dose and SARS-CoV-2 viral inactivation across N95 respirators during decontamination by Geldert, Alisha, Su, Alison, Roberts, Allison W., Golovkine, Guillaume, Grist, Samantha M., Stanley, Sarah A., Herr, Amy E.

“…During public health crises like the COVID-19 pandemic, ultraviolet-C (UV-C) decontamination of N95 respirators for emergency reuse has been implemented to…”
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