Search Results - "Hernández, Karel"

Nucleophile Promiscuity of Engineered Class II Pyruvate Aldolase YfaU from E. Coli by Hernández, Karel, Joglar, Jesús, Bujons, Jordi, Parella, Teodor, Clapés, Pere

“…Pyruvate‐dependent aldolases exhibit a stringent selectivity for pyruvate, limiting application of their synthetic potential, which is a drawback shared with…”
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Unifying Scheme for the Biosynthesis of Acyl‐Branched Sugars: Extended Substrate Scope of Thiamine‐Dependent Enzymes by Steitz, Jan‐Patrick, Krug, Leonhard, Walter, Lydia, Hernández, Karel, Röhr, Caroline, Clapés, Pere, Müller, Michael

“…Thiamine diphosphate (ThDP) dependent enzymes are useful catalysts for asymmetric C−C bond formation through benzoin‐type condensation reactions that result in…”
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Expanding Synthetic Applications of Δ1‐Piperidine‐2‐carboxylate/Δ1‐pyrroline‐2‐carboxylate Reductase from Pseudomonas syringae (DpkAPsyrin). Biocatalytic Asymmetric Synthesis of (S,E)‐2‐hydroxy‐4‐arylbut‐3‐enoic Acid Derivatives by Moreno, Carlos J., Gittings, Samantha, Schollmeyer, Dieter, Joglar, Jesús, Bujons, Jordi, Hernández, Karel, Clapés, Pere

“…Chiral 2‐hydroxy‐4‐arylbut‐3‐enoic acid derivatives are important precursors for the synthesis of angiotensin converting enzyme (ACE) inhibitors, such as…”
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Nucleophile Promiscuity of Natural and Engineered Aldolases by Hernández, Karel, Szekrenyi, Anna, Clapés, Pere

“…The asymmetric aldol addition reaction mediated by aldolases is recognized as a green and sustainable method for carbon–carbon bond formation. Research in this…”
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Use of temporary immersion systems for the micropropagation of Puya alpestris (Poepp.) Gay as a source to produce proteolytic enzymes by Cabrera, Inelvis Castro, Hernández, Karel Vives, Burgos, Luciano Sotomayor, de la Torre, Martha Hernández

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2‐Keto‐3‐Deoxy‐l‐Rhamnonate Aldolase (YfaU) as Catalyst in Aldol Additions of Pyruvate to Amino Aldehyde Derivatives by Hernández, Karel, Gómez, Ariadna, Joglar, Jesús, Bujons, Jordi, Parella, Teodor, Clapés, Pere

“…4‐Hydroxy‐2‐keto acid derivatives are versatile building blocks for the synthesis of amino acids, hydroxy carboxylic acids and chiral aldehydes. Pyruvate…”
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Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants by Hernández, Karel Vives, Moreno-Romero, Jordi, Hernández de la Torre, Martha, Manríquez, Claudia Pérez, Leal, Darcy Ríos, Martínez-Garcia, Jaime F.

“…Stevia rebaudiana leaf extracts contain stevioside and rebaudioside A, two steviol glycosides (SGs) used as natural sweeteners because of their non-toxic,…”
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In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases by Castro Cabrera, Inelvis, Vives Hernández, Karel, Bruno, Mariela Anahí, Obregón, Walter David, Hernández de la Torre, Martha

“…Puya chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent…”
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Chemoenzymatic Hydroxymethylation of Carboxylic Acids by Tandem Stereodivergent Biocatalytic Aldol Reaction and Chemical Decarboxylation by Marín-Valls, Roser, Hernández, Karel, Bolte, Michael, Joglar, Jesús, Bujons, Jordi, Clapés, Pere

“…Chiral 2-substituted 3-hydroxycarboxylic acid derivatives are valuable building blocks for the preparation of naturally occurring and synthetic biologically…”
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Expedient Synthesis of C-Aryl Carbohydrates by Consecutive Biocatalytic Benzoin and Aldol Reactions by Hernández, Karel, Parella, Teodor, Joglar, Jesús, Bujons, Jordi, Pohl, Martina, Clapés, Pere

“…The introduction of aromatic residues connected by a CC bond into the non‐reducing end of carbohydrates is highly significant for the development of…”
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Accessing to the Nicotiana tabacum leaf antimicrobial activity: In-silico and in-vitro investigations by Capdesuñer, Yanelis, García-Brizuela, Jorge, Mock, Hans Peter, Hernández, Karel Vives, de la Torre, Martha Hernández, Santiesteban-Toca, Cosme E.

“…In this research, in-silico and in-vitro approaches were adopted with the aim to investigate the relationship between the tobacco leaf structures (trichomes)…”
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Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance by Hernandez, Karel, Fernandez-Lafuente, Roberto

“…Mutagenesis and immobilization are usually considered to be unrelated techniques with potential applications to improve protein properties. However, there are…”
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Synthesis of γ‑Hydroxy-α-amino Acid Derivatives by Enzymatic Tandem Aldol Addition–Transamination Reactions by Moreno, Carlos J, Hernández, Karel, Charnok, Simon J, Gittings, Samantha, Bolte, Michael, Joglar, Jesús, Bujons, Jordi, Parella, Teodor, Clapés, Pere

“…Three enzymatic routes toward γ-hydroxy-α-amino acids by tandem aldol addition–transamination one-pot two-step reactions are reported. The approaches feature…”
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Preparation of thermostable trypsin–polysaccharide neoglycoenzymes through Ugi multicomponent reaction by García, Ariel, Hernández, Karel, Chico, Belkis, García, Daniel, Villalonga, Maria L., Villalonga, Reynaldo

“…A novel synthetic method for preparing enzyme-polysaccharide derivatives is described, based on the use of the Ugi multicomponent reaction. Bovine pancreatic…”
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Biocatalytic Construction of Quaternary Centers by Aldol Addition of 3,3-Disubstituted 2‑Oxoacid Derivatives to Aldehydes by Marín-Valls, Roser, Hernández, Karel, Bolte, Michael, Parella, Teodor, Joglar, Jesús, Bujons, Jordi, Clapés, Pere

“…The congested nature of quaternary carbons hinders their preparation, most notably when stereocontrol is required. Here we report a biocatalytic method for the…”
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Biocatalytic Synthesis of Homochiral 2‑Hydroxy-4-butyrolactone Derivatives by Tandem Aldol Addition and Carbonyl Reduction by Moreno, Carlos J., Hernández, Karel, Gittings, Samantha, Bolte, Michael, Joglar, Jesús, Bujons, Jordi, Parella, Teodor, Clapés, Pere

“…Chiral 2-hydroxy acids and 2-hydroxy-4-butyrolactone derivatives are structural motifs often found in fine and commodity chemicals. Here, we report a tandem…”
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Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene–divinylbenzene beads by Hernandez, Karel, Garcia-Galan, Cristina, Fernandez-Lafuente, Roberto

“…Two commercial porous styrene–divinylbenzene beads (Diaion HP20LX and MCI GEL CHP20P) have been evaluated as supports to immobilize lipase B from Candida…”
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Cascade Synthesis of l‑Homoserine Catalyzed by Lyophilized Whole Cells Containing Transaminase and Aldolase Activities: The Mathematical Modeling Approach by Česnik Katulić, Morana, Sudar, Martina, Hernández, Karel, Qi, Yuyin, Charnock, Simon J, Vasić-Rački, Đurdica, Clapés, Pere, Findrik Blažević, Zvjezdana

“…Aldolase and transaminase coexpressed in Escherichia coli cells and lyophilized (i.e., lyophilized whole-cell biocatalyst (LWCB)) were used as biocatalysts for…”
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Prosthetic valve thrombosis: A regimen of treatment with low-dose and longer-course using recombinant tissue-type plasminogen activator is a promising protocol by Cáceres-Lóriga, Fidel Manuel, MD, PhD, Pérez-López, Horacio, MD, PhD, Morlans-Hernández, Karel, MD

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Aldolase‐Catalyzed Asymmetric Synthesis of N‐Heterocycles by Addition of Simple Aliphatic Nucleophiles to Aminoaldehydes by Roldán, Raquel, Hernández, Karel, Joglar, Jesús, Bujons, Jordi, Parella, Teodor, Fessner, Wolf‐Dieter, Clapés, Pere

“…Nitrogen heterocycles are structural motifs found in many bioactive natural products and of utmost importance in pharmaceutical drug development. In this work,…”
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