Search Results - "Hernáez, María Luisa"
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General Statistical Framework for Quantitative Proteomics by Stable Isotope Labeling
Published in Journal of proteome research (07-03-2014)“…The combination of stable isotope labeling (SIL) with mass spectrometry (MS) allows comparison of the abundance of thousands of proteins in complex mixtures…”
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Phosphoproteomic Analysis of Protein Kinase C Signaling in Saccharomyces cerevisiae Reveals Slt2 Mitogen-activated Protein Kinase (MAPK)-dependent Phosphorylation of Eisosome Core Components
Published in Molecular & cellular proteomics (01-03-2013)“…The cell wall integrity (CWI) pathway of the model organism Saccharomyces cerevisiae has been thoroughly studied as a paradigm of the mitogen-activated protein…”
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Unravelling the Role of Candida albicans Prn1 in the Oxidative Stress Response through a Proteomics Approach
Published in Antioxidants (26-04-2024)“…Prn1 is a protein with an unknown function similar to mammalian Pirin. It also has orthologues in other pathogenic fungi, but not in . Prn1 highly increases…”
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Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis
Published in Frontiers in microbiology (05-07-2021)“…The use of metaproteomics for studying the human gut microbiota can shed light on the taxonomic profile and the functional role of the microbial community…”
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Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans
Published in Journal of proteome research (03-05-2019)“…Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways are activated, leading to the…”
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Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord
Published in Journal of proteome research (07-12-2018)“…Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human…”
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Dual Regulation of the mitotic exit network (MEN) by PP2A-Cdc55 phosphatase
Published in PLoS genetics (01-12-2013)“…Exit from mitosis in budding yeast is triggered by activation of the key mitotic phosphatase Cdc14. At anaphase onset, the protease separase and Zds1 promote…”
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Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approach
Published in Journal of proteomics (07-05-2010)“…We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of…”
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Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins
Published in Journal of proteomics (18-04-2010)“…The study of Saccharomyces cerevisiae cell surface proteins was performed because of their important role in cell wall biogenesis and in the physiology of the…”
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Journal Article Conference Proceeding -
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Surfing Transcriptomic Landscapes. A Step beyond the Annotation of Chromosome 16 Proteome
Published in Journal of proteome research (03-01-2014)“…The Spanish team of the Human Proteome Project (SpHPP) marked the annotation of Chr16 and data analysis as one of its priorities. Precise annotation of…”
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Candida albicans cell shaving uncovers new proteins involved in cell wall integrity, yeast to hypha transition, stress response and host–pathogen interaction
Published in Journal of proteomics (08-09-2015)“…The ability to switch from yeast to hyphal growth is essential for virulence in Candida albicans. The cell surface is the initial point of contact between the…”
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Candida albicans Hyphal Extracellular Vesicles Are Different from Yeast Ones, Carrying an Active Proteasome Complex and Showing a Different Role in Host Immune Response
Published in Microbiology spectrum (01-06-2022)“…Candida albicans is the principal causative agent of lethal fungal infections, predominantly in immunocompromised hosts. Extracellular vesicles (EVs) have been…”
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SILAC-based phosphoproteomics reveals new PP2A-Cdc55-regulated processes in budding yeast
Published in Gigascience (01-05-2018)“…Protein phosphatase 2A (PP2A) is a family of conserved serine/threonine phosphatases involved in several essential aspects of cell growth and proliferation…”
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Combined Proteomic Approaches for the Identification of Specific Amino Acid Residues Modified by 4-Hydroxy-2-Nonenal under Physiological Conditions
Published in Journal of proteome research (05-11-2010)“…Proteins modified by 4-hydroxy-2-nonenal (HNE) are cellular markers of oxidative stress in health and disease. HNE is generated by free radical chain reactions…”
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Guidelines for reporting quantitative mass spectrometry based experiments in proteomics
Published in Journal of proteomics (16-12-2013)“…Mass spectrometry is already a well-established protein identification tool and recent methodological and technological developments have also made possible…”
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SILAC-Based Quantitative Phosphoproteomics in Yeast
Published in Methods in molecular biology (Clifton, N.J.) (2023)“…In this chapter, detailed procedures for stable isotope labeling with amino acids in cell culture, SILAC labeling of yeast auxotroph, optimization and…”
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Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye
Published in BMC microbiology (25-11-2015)“…Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and…”
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Unravelling the Role of ICandida albicans/I Prn1 in the Oxidative Stress Response through a Proteomics Approach
Published in Antioxidants (01-05-2024)“…Candida albicans Prn1 is a protein with an unknown function similar to mammalian Pirin. It also has orthologues in other pathogenic fungi, but not in…”
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Quantitative Proteome and Acidic Subproteome Profiling of Candida albicans Yeast-to-Hypha Transition
Published in Journal of proteome research (04-02-2011)“…Candida albicans yeast-to-hypha morphological transition is involved in the virulence strategy of this opportunistic fungal pathogen. Changes in relative…”
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In Vitro Transcription/Translation System: A Versatile Tool in the Search for Missing Proteins
Published in Journal of proteome research (04-09-2015)“…Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria…”
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