Mechanotransducers in Rat Pulpal Afferents

The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal affere...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of dental research Vol. 87; no. 9; pp. 834 - 838
Main Authors:	Hermanstyne, T.O., Markowitz, K., Fan, L., Gold, M.S.
Format:	Journal Article
Language:	English
Published:	United States SAGE Publications 01-09-2008 SAGE PUBLICATIONS, INC
Subjects:	Acid Sensing Ion Channels Animals Dental Pulp - cytology Dental Pulp - innervation Dental Pulp - metabolism Dental Pulp Exposure - metabolism Dentistry Male Maxilla Mechanoreceptors - physiology Molar Nerve Fibers - metabolism Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neural Conduction - physiology Neurons, Afferent - cytology Neurons, Afferent - metabolism Rats Rats, Sprague-Dawley RNA, Messenger - analysis Signal Transduction - physiology Sodium Channels - genetics Sodium Channels - metabolism Trigeminal Ganglion - cytology Trigeminal Ganglion - metabolism dentin hypersensitivity single-cell PCR retrograde labeling primary afferent
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal afferents, we used a single-cell PCR approach to screen retrogradely labeled pulpal afferents. The presence of mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the α, β, and γ subunits of ENaC, and the two pore K+ channels (TREK1, TREK2) and TRAAK were screened in pulpal neurons from rats with and without pulpal inflammation. ASIC3, TRPA1, TREK1, and TREK2 were present in ~67%, 64%, 14%, and 10% of pulpal neurons, respectively. There was no detectable influence of inflammation on the proportion of neurons expressing these mechanotransducers. Given that the majority of pulpal afferents express ASIC3 and TRPA1, our results raise the possibility that these channels may be novel targets for the treatment of dentin sensitivity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-0345 1544-0591
DOI:	10.1177/154405910808700910