Potato virus X TGBp1 induces plasmodesmata gating and moves between cells in several host species whereas CP moves only in N. benthamiana leaves

Experiments were conducted to compare the plasmodesmal transport activities of Potato virus X (PVX) TGBp1 and coat protein (CP) in several plant species. Microinjection experiments indicated that TGBp1 gates plasmodesmata in Nicotiana tabacum leaves. These results support previous microinjection stu...

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Bibliographic Details
Published in:	Virology (New York, N.Y.) Vol. 328; no. 2; pp. 185 - 197
Main Authors:	Howard, Amanda R., Heppler, Marty L., Ju, Ho-Jong, Krishnamurthy, Konduru, Payton, Mark E., Verchot-Lubicz, Jeanmarie
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 25-10-2004
Subjects:	60 APPLIED LIFE SCIENCES Adenosine Triphosphatases - metabolism Amino Acid Motifs Capsid Proteins - metabolism coat proteins GENES Green Fluorescent Proteins host-pathogen relationships LEAVES Lycopersicon esculentum MUTANTS Mutation MUTATIONS NICOTIANA Nicotiana - virology Nicotiana benthamiana Nicotiana clevelandii Nicotiana tabacum Plant Leaves - metabolism Plant Leaves - virology PLASMIDS plasmodesmata Plasmodesmata - metabolism Potato virus X POTATOES Potexvirus - metabolism Protein Transport PROTEINS TGB TOBACCO VIRAL DISEASES viral proteins Viral Proteins - genetics Viral Proteins - metabolism virus movement VIRUSES Potato virus X Nicotiana benthamiana TGB
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Summary:	Experiments were conducted to compare the plasmodesmal transport activities of Potato virus X (PVX) TGBp1 and coat protein (CP) in several plant species. Microinjection experiments indicated that TGBp1 gates plasmodesmata in Nicotiana tabacum leaves. These results support previous microinjection studies indicating that TGBp1 gates plasmodesmata in Nicotiana benthamiana and Nicotiana clevelandii leaves. To study protein movement, plasmids expressing the green fluorescent protein (GFP) gene fused to the PVX TGBp1 or CP genes were biolistically bombarded to leaves taken from four different PVX host species. GFP/TGBp1 moved between adjacent cells in N. tabacum, N. clevelandii, N. benthamiana, and Lycopersicon esculentum, whereas GFP/CP moved only in N. benthamiana leaves. Mutations m12 and m13 were introduced into the TGBp1 gene and both mutations eliminated TGBp1 ATPase active site motifs, inhibited PVX movement, reduced GFP/TGBp1 cell-to-cell movement in N. benthamiana leaves, and eliminated GFP/TGBp1 movement in N. tabacum, N. clevelandii, and L. esculentum leaves. GFP/TGBp1m13 formed aggregates in tobacco cells. The ability of GFP/CP and mutant GFP/TGBp1 fusion proteins to move in N. benthamiana and not in the other PVX host species suggests that N. benthamiana plants have a unique ability to promote protein intercellular movement.
Bibliography:	http://www.sciencedirect.com/science/journal/00426822 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0042-6822 1096-0341
DOI:	10.1016/j.virol.2004.06.039