Search Results - "Hepler, J R"

Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases by TAUSSIG, R, WEI-JEN TANG, HEPLER, J. R, GILMAN, A. G

“…The capacities of the alpha subunits of pertussis toxin-sensitive guanine nucleotide-binding regulatory proteins (G proteins) to inhibit different isoforms of…”
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RGS14 limits seizure-induced mitochondrial oxidative stress and pathology in hippocampus by Harbin, N.H., Lustberg, D.J., Hurst, C., Pare, J., Crotty, K.M., Waters, A.L., Yeligar, S.M., Smith, Y., Seyfried, N.T., Weinshenker, D., Hepler, J.R.

“…RGS14 is a complex multifunctional scaffolding protein that is highly enriched within pyramidal cells (PCs) of hippocampal area CA2. In these neurons, RGS14…”
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Lipid modifications of G proteins: alpha subunits are palmitoylated by LINDER, M. E, MIDDLETON, P, HEPLER, J. R, TAUSSIG, R, GILMAN, A. G, MUMBY, S. M

“…A small number of membrane-associated proteins are reversibly and covalently modified with palmitic acid. Palmitoylation of G-protein alpha and beta subunits…”
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Purification from Sf9 cells and characterization of recombinant Gq alpha and G11 alpha. Activation of purified phospholipase C isozymes by G alpha subunits by HEPLER, J. R, KOZASA, T, SMRCKA, A. V, SIMON, M. I, SUE GOO RHEE, STERNWEIS, P. C, GILMAN, A. G

“…Members of the Gq alpha subfamily of heterotrimeric guanine nucleotide-binding proteins (G proteins) activate phospholipase C (PLC). The complementary DNAs…”
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Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq by SMRCKA, A. V, HEPLER, J. R, BROWN, K. O, STERNWEIS, P. C

“…The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and…”
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Long-term phorbol ester treatment down-regulates protein kinase C and sensitizes the phosphoinositide signaling pathway to hormone and growth factor stimulation. Evidence for a role of protein kinase C in agonist-induced desensitization by Hepler, J R, Earp, H S, Harden, T K

“…Exposure of a nontransformed, continuous line of epithelial cells derived from rat liver (WB cells) to epidermal growth factor, angiotensin II,…”
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Characterization of antisera to glutamate and aspartate by Hepler, JR, Toomim, CS, McCarthy, KD, Conti, F, Battaglia, G, Rustioni, A, Petrusz, P

“…Antisera were raised in rabbits against glutamate (Glu) and aspartate (Asp) conjugated to the invertebrate carrier protein hemocyanin (HC) with glutaraldehyde…”
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Purification and Characterization of Recombinant G16αFrom Sf9 Cells: Activation of Purified Phospholipase C Isozymes by G-Protein α Subunits by KOZASA, T, HEPLER, J. R, SMRCKA, A. V, SIMON, M. I, SUE GOO RHEE, STERNWEIS, P. C, GILMAN, A. G

“…A cDNA encoding G16α, the α subunit of a heterotrimeric guanine nucleotide-binding protein, was expressed in Sf9 cells using recombinant baculovirus. G16αin…”
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Epidermal growth factor stimulates the rapid accumulation of inositol (1,4,5)-trisphosphate and a rise in cytosolic calcium mobilized from intracellular stores in A431 cells by Hepler, J.R., Nakahata, N., Lovenberg, T.W., DiGuiseppi, J., Herman, B., Earp, H.S., Harden, T.K.

“…Exposure of A431 human epidermoid carcinoma cells to epidermal growth factor (EGF), bradykinin, and histamine resulted in a time- and concentration-dependent…”
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G proteins by Hepler, J R, Gilman, A G

“…The family of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) serves an essential role in transducing receptor-generated signals…”
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Epidermal growth factor (EGF) and hormones stimulate phosphoinositide hydrolysis and increase EGF receptor protein synthesis and mRNA levels in rat liver epithelial cells. Evidence for protein kinase C-dependent and -independent pathways by Earp, H S, Hepler, J R, Petch, L A, Miller, A, Berry, A R, Harris, J, Raymond, V W, McCune, B K, Lee, L W, Grisham, J W

“…Epidermal growth factor (EGF) stimulated the rapid accumulation of inositol trisphosphate in WB cells, a continuous line of rat hepatic epithelial cells. Since…”
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RGS7 Is Palmitoylated and Exists as Biochemically Distinct Forms by Rose, Jeremy J., Taylor, Jackie B., Shi, Jing, Cockett, Mark I., Jones, Philip G., Hepler, John R.

“…: Regulator of G protein signaling (RGS) proteins are GTPase‐activating proteins that modulate neurotransmitter and G protein signaling. RGS7 and its binding…”
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H1-histamine receptors on human astrocytoma cells by Nakahata, N, Martin, M W, Hughes, A R, Hepler, J R, Harden, T K

“…The H1-histamine receptor antagonist [3H]mepyramine bound with high affinity (Kd = 3-5 nM) to membranes derived from 1321N1 human astrocytoma cells. The…”
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Inhibition of early platelet-derived growth factor responses in BALB/c-3T3 cells by interferon by Zagari, M, Hepler, J R, Harris, C, Herman, B

“…Stimulation of total inositol phosphate production, alteration of cytosolic free calcium [( Ca++]i), vinculin disruption from adhesion plaques, and DNA…”
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Profile of amino acid synthesis in rat hippocampus during push-pull perfusion of ethanol or morphine by Noto, T, Hepler, J R, Myers, R D

“…In the unanesthetized rat, the effect of ethanol or morphine on the new synthesis of six amino acids, gamma-aminobutyrate, glutamate, alanine, aspartate,…”
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Neurotensin releases norepinephrine differentially from perfused hypothalamus of sated and fasted rat by Lee, T F, Rezvani, A H, Hepler, J R, Myers, R D

“…The central injection of neurotensin (NT) has been reported to attenuate the intake of food in the fasted animal. To determine whether endogenous…”
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Emerging roles for RGS proteins in cell signalling by Hepler, John R

“…Regulators of G-protein signalling (RGS proteins) are a family of highly diverse, multifunctional signalling proteins that share a conserved 120 amino acid…”
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Glass-plastic push-pull cannula system for regional perfusion of sites in the brain by Myers, R D, Hepler, J R

“…A new non-metallic push-pull cannula device is described for the localized perfusion of brain sites in the rat or other animal. Comprised entirely of glass and…”
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Guanine nucleotide-dependent pertussis-toxin-insensitive stimulation of inositol phosphate formation by carbachol in a membrane preparation from human astrocytoma cells by Hepler, J R, Harden, T K

“…The efficacy of muscarinic-receptor agonists for stimulation of inositol phosphate formation and Ca2+ mobilization in intact 1321N1 human astrocytoma cells is…”
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Evaluation of neurotensin's thermolytic action by ICV infusion with receptor antagonists and a Ca++ chelator by Lee, T F, Hepler, J R, Myers, R D

“…To investigate the mechanisms responsible for the thermolytic action of neurotensin (NT), cannulae for intracerebroventricular (ICV) injection were implanted…”
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