C26 ASTHMA IN INFANTS AND CHILDREN: The Anthelminthic Niclosamide And Related Compounds Represent Potent Tmem16a Antagonists That Fully Relax Mouse And Human Airway Rings

Aside from using the wire myograph system as a late stage screening tool, we employ wire myograph to explore various aspects of human disease such as: 1) mimicking severe disease with strong stimuli (challenge rings with an EC95 level of carbachol), 2) assessing the impact of inflammation (overnight...

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Bibliographic Details
Published in:	American journal of respiratory and critical care medicine Vol. 195
Main Authors:	Miner, K, Mohn, D, Elliott, R, Powers, D, Chen, J, Liu, B, Wang, P, Gaida, K, Hochheimer, A, Liu, L, Henckels, K, Labitzke, K, Sullivan, J K
Format:	Journal Article
Language:	English
Published:	New York American Thoracic Society 01-01-2017
Subjects:	Asthma Inflammation Rodents
Online Access:	Get full text
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Summary:	Aside from using the wire myograph system as a late stage screening tool, we employ wire myograph to explore various aspects of human disease such as: 1) mimicking severe disease with strong stimuli (challenge rings with an EC95 level of carbachol), 2) assessing the impact of inflammation (overnight treatment with cytokine cocktail and subsequent contractile challenge + Isoproterenol (ß-agonist) +/- TMEM16A Antagonist, 3) Use-dependent desensitization where a ß-agonist can be used repeatedly yielding decreased activity, 4) or whether a drug candidate is short or long acting. [...]two benchmark compounds displayed different abilities to maintain relaxation after washout and re-challenge with a contractile.
ISSN:	1073-449X 1535-4970