Evaluation of the ERIC-PCR as a probable method to differentiate Avibacterium paragallinarum serovars

Infectious coryza, an upper respiratory tract disease in chickens, caused by Avibacterium paragallinarum, leads to huge economic losses. The disease is controlled through vaccination; but vaccination efficacy is dependent on correct identification of the infecting serovar, as limited cross-protectio...

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Bibliographic Details
Published in:	Avian pathology Vol. 46; no. 3; pp. 272 - 277
Main Authors:	Hellmuth, Julius Eduard, Hitzeroth, Arina Corli, Bragg, Robert Richard, Boucher, Charlotte Enastacia
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 04-05-2017 Taylor & Francis Ltd
Subjects:	Animals Avibacterium paragallinarum Chickens - microbiology DNA, Intergenic - genetics Enterobacteriaceae - genetics Enterobacteriaceae - immunology Enterobacteriaceae - isolation & purification ERIC-PCR Genes Haemophilus paragallinarum - genetics Haemophilus paragallinarum - immunology Haemophilus paragallinarum - isolation & purification Immunization molecular typing Molecular Typing - veterinary Multiplex Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Poultry Diseases - diagnosis Poultry Diseases - microbiology Repetitive Sequences, Nucleic Acid - genetics Reproducibility of Results Serogroup serovar differentiation Species Specificity species-specific PCR Avibacterium paragallinarum species-specific PCR ERIC-PCR molecular typing serovar differentiation
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Summary:	Infectious coryza, an upper respiratory tract disease in chickens, caused by Avibacterium paragallinarum, leads to huge economic losses. The disease is controlled through vaccination; but vaccination efficacy is dependent on correct identification of the infecting serovar, as limited cross-protection is reported amongst some serovars. Current identification methods include the heamagglutination inhibition test, which is demanding and could be subjective. To overcome this, molecular typing methods proposed are the Multiplex polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism-PCR, but low reproducibility is reported. Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR has been suggested for molecular groupings of various bacterial species. This study focuses on evaluating the ERIC-PCR as a probable method to differentiate between different Av. paragallinarum serovars by grouping with reference isolates, based on clonal relations. The ERIC-PCR was performed on 12 reference isolates and 41 field isolates originating from South Africa and South America. The data indicate that the ERIC-PCR is not ideal for the differentiation or for molecular typing of Av. paragallinarum serovars, as no correlation is drawn upon comparison of banding patterns of field isolates and reference strains. However, the results do indicate isolates from the same origin sharing unique banding patterns, indicating potential clonal relationship; but when compared to the reference isolates dominant in the specific area, no correlation could be drawn. Furthermore, although the ERIC-PCR serves a purpose in epidemiological studies, it has proved to have little application in differentiating amongst serovars of Av. paragallinarum and to group untyped field strains with known reference strains.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0307-9457 1465-3338
DOI:	10.1080/03079457.2016.1259610