Search Results - "Helinski, Donald R"

ParE toxin encoded by the broad‐host‐range plasmid RK2 is an inhibitor of Escherichia coli gyrase by Jiang, Yong, Pogliano, Joe, Helinski, Donald R., Konieczny, Igor

“…Summary Broad‐host‐range plasmid RK2 encodes a post‐segregational killing system, parDE, which contributes to the stable maintenance of this plasmid in…”
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A Brief History of Plasmids by Helinski, Donald R

“…In the late 1950s, a number of laboratories took up the study of plasmids once the discovery was made that extrachromosomal antibiotic resistance (R) factors…”
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Localization of the Naturally Occurring Plasmid ColE1 at the Cell Pole by SHIYIN YAO, HELINSKI, Donald R, TOUKDARIAN, Aresa

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Multicopy Plasmids are Clustered and Localized in Escherichia coli by Pogliano, Joe, Ho, Thanh Quoc, Zhong, Zhenping, Helinski, Donald R.

“…We localized the multicopy plasmid RK2 in Escherichia coli and found that the number of fluorescent foci observed in each cell was substantially less than the…”
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A Multifunctional Plasmid-Encoded Replication Initiation Protein Both Recruits and Positions an Active Helicase at the Replication Origin by Jiang, Yong, Pacek, Marcin, Helinski, Donald R., Konieczny, Igor, Toukdarian, Aresa

“…The DnaA replication initiation protein has been shown to be essential for DNA strand opening at the AT-rich region of the replication origin of the…”
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Gyrase Inhibitors and Thymine Starvation Disrupt the Normal Pattern of Plasmid RK2 Localization in Escherichia coli by JOHNSON, Erik P, SHIYIN YAO, HELINSKI, Donald R

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The Replication Initiation Protein of the Broad-Host-Range Plasmid RK2 is Activated by the ClpX Chaperone by Konieczny, I, Helinski, D R

“…Initiation and control of replication of the broad-host-range plasmid RK2 requires two plasmid-encoded elements, the replication origin (oriV) and the…”
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Isolation of Broad-Host-Range Replicons from Marine Sediment Bacteria by SOBECKY, P. A, MINCER, T. J, CHANG, M. C, TOUKDARIAN, A, HELINSKI, D. R

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Role of the parCBA Operon of the Broad-Host-Range Plasmid RK2 in Stable Plasmid Maintenance by Easter, C L, Schwab, H, Helinski, D R

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Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants by Ow, D.W, Wood, K.V, DeLuca, M, De Wet, J.R, Helinski, D.R, Howell, S.H

“…The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic…”
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Plasmid RK2 ParB Protein: Purification and Nuclease Properties by Johnson, E P, Mincer, T, Schwab, H, Burgin, A B, Helinski, D R

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A broad host range replicon with different requirements for replication initiation in three bacterial species by Caspi, Ron, Pacek, Marcin, Consiglieri, Giac, Helinski, Donald R., Toukdarian, Aresa, Konieczny, Igor

“…Plasmid RK2 is unusual in its ability to replicate stably in a wide range of Gram‐negative bacteria. The replication origin (oriV) and a plasmid‐encoded…”
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The incC korB region of RK2 repositions a mini-RK2 replicon in Escherichia coli by Verheust, Celine, Helinski, Donald R.

“…Analysis by fluorescence microscopy has established that plasmid RK2 in Escherichia coli and other gram-negative bacteria is present as discrete clusters that…”
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A Brief History of Plasmids by Helinski, Donald R

“…In the late 1950s, a number of laboratories took up the study of plasmids once the discovery was made that extrachromosomal antibiotic resistance (R) factors…”
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haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti by Gilles-Gonzalez, M.A, Ditta, G.S, Helinski, D.R

“…The expression of the nitrogen-fixation genes of Rhizobium meliloti is controlled by oxygen. These genes are induced when the free oxygen concentration is…”
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Helicase Delivery and Activation by DnaA and TrfA Proteins during the Initiation of Replication of the Broad Host Range Plasmid RK2 by Konieczny, Igor, Helinski, Donald R.

“…Specific binding of the plasmid-encoded protein, TrfA, and the Escherichia coli DnaA protein to the origin region (oriV) is required for the initiation of…”
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Role of TrfA and DnaA Proteins in Origin Opening during Initiation of DNA Replication of the Broad Host Range Plasmid RK2 by Konieczny, Igor, Doran, Kelly S., Helinski, Donald R., Blasina, Alessandra

“…The Escherichia coli protein DnaA and the plasmid RK2-encoded TrfA protein are required for initiation of replication of the broad host range plasmid RK2. The…”
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Inhibition of protein and RNA synthesis in Escherichia coli results in declustering of plasmid RK2 by Yao, Shiyin, Toukdarian, Aresa, Helinski, Donald R.

“…Multi-copy plasmids in Escherichia coli are not randomly distributed throughout the cell but are present as clusters of plasmid molecules that are localized at…”
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Interactions of DnaA Proteins from Distantly Related Bacteria with the Replication Origin of the Broad Host Range Plasmid RK2 by Caspi, Ron, Helinski, Donald R., Pacek, Marcin, Konieczny, Igor

“…Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its…”
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Plasmids isolated from marine sediment microbial communities contain replication and incompatibility regions unrelated to those of known plasmid groups by SOBECKY, P. A, MINCER, T. J, CHANG, M. C, HELINSKI, D. R

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