Search Results - "Helge Ewers"

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies by Ries, Jonas, Kaplan, Charlotte, Platonova, Evgenia, Eghlidi, Hadi, Ewers, Helge

“…Nanobodies that bind to fluorescent proteins with high affinity and are coupled to bright organic dyes allow simple efficient labeling of fusion proteins for…”
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Expansion Stimulated Emission Depletion Microscopy (ExSTED) by Gao, Mengfei, Maraspini, Riccardo, Beutel, Oliver, Zehtabian, Amin, Eickholt, Britta, Honigmann, Alf, Ewers, Helge

“…Stimulated emission depletion (STED) microscopy is routinely used to resolve the ultrastructure of cells with a ∼10-fold higher resolution compared to…”
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Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast by Kaplan, Charlotte, Ewers, Helge

“…Preserving cell structure and protein localization during sample preparation is crucial for accurate superresolution microscopy. This protocol uses cell…”
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Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican by Favuzzi, Emilia, Marques-Smith, André, Deogracias, Rubén, Winterflood, Christian M., Sánchez-Aguilera, Alberto, Mantoan, Laura, Maeso, Patricia, Fernandes, Cathy, Ewers, Helge, Rico, Beatriz

“…Activity-dependent neuronal plasticity is a fundamental mechanism through which the nervous system adapts to sensory experience. Several lines of evidence…”
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Directed manipulation of membrane proteins by fluorescent magnetic nanoparticles by Li, Jia Hui, Santos-Otte, Paula, Au, Braedyn, Rentsch, Jakob, Block, Stephan, Ewers, Helge

“…The plasma membrane is the interface through which cells interact with their environment. Membrane proteins are embedded in the lipid bilayer of the plasma…”
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An open-source, high-resolution, automated fluorescence microscope by Zehrer, Ando Christian, Martin-Villalba, Ana, Diederich, Benedict, Ewers, Helge

“…Fluorescence microscopy is a fundamental tool in the life sciences, but the availability of sophisticated equipment required to yield high-quality,…”
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Unblending of Transcriptional Condensates in Human Repeat Expansion Disease by Basu, Shaon, Mackowiak, Sebastian D., Niskanen, Henri, Knezevic, Dora, Asimi, Vahid, Grosswendt, Stefanie, Geertsema, Hylkje, Ali, Salaheddine, Jerković, Ivana, Ewers, Helge, Mundlos, Stefan, Meissner, Alexander, Ibrahim, Daniel M., Hnisz, Denes

“…Expansions of amino acid repeats occur in >20 inherited human disorders, and many occur in intrinsically disordered regions (IDRs) of transcription factors…”
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Binding-Activated Localization Microscopy of DNA Structures by Schoen, Ingmar, Ries, Jonas, Klotzsch, Enrico, Ewers, Helge, Vogel, Viola

“…Many nucleic acid stains show a strong fluorescence enhancement upon binding to double-stranded DNA. Here we exploit this property to perform superresolution…”
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High-speed nanoscopic tracking of the position and orientation of a single virus by Helenius, Ari, Müller, Christian, Ewers, Helge, Renn, Alois, Kukura, Philipp, Sandoghdar, Vahid

“…A combination of scattering interferometry and single-molecule fluorescence microscopy allows visualization of both the position and orientation of single…”
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Nanoscopic compartmentalization of membrane protein motion at the axon initial segment by Albrecht, David, Winterflood, Christian M, Sadeghi, Mohsen, Tschager, Thomas, Noé, Frank, Ewers, Helge

“…The axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal, and transmembrane molecules. During AIS establishment, a membrane diffusion…”
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Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells by Gabriel, Christian H., del Olmo, Marta, Zehtabian, Amin, Jäger, Marten, Reischl, Silke, van Dijk, Hannah, Ulbricht, Carolin, Rakhymzhan, Asylkhan, Korte, Thomas, Koller, Barbara, Grudziecki, Astrid, Maier, Bert, Herrmann, Andreas, Niesner, Raluca, Zemojtel, Tomasz, Ewers, Helge, Granada, Adrián E., Herzel, Hanspeter, Kramer, Achim

“…The cell biology of circadian clocks is still in its infancy. Here, we describe an efficient strategy for generating knock-in reporter cell lines using CRISPR…”
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Synapsin condensation controls synaptic vesicle sequestering and dynamics by Hoffmann, Christian, Rentsch, Jakob, Tsunoyama, Taka A., Chhabra, Akshita, Aguilar Perez, Gerard, Chowdhury, Rajdeep, Trnka, Franziska, Korobeinikov, Aleksandr A., Shaib, Ali H., Ganzella, Marcelo, Giannone, Gregory, Rizzoli, Silvio O., Kusumi, Akihiro, Ewers, Helge, Milovanovic, Dragomir

“…Neuronal transmission relies on the regulated secretion of neurotransmitters, which are packed in synaptic vesicles (SVs). Hundreds of SVs accumulate at…”
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High-Speed Single-Particle Tracking of GM1 in Model Membranes Reveals Anomalous Diffusion due to Interleaflet Coupling and Molecular Pinning by Spillane, Katelyn M, Ortega-Arroyo, Jaime, de Wit, Gabrielle, Eggeling, Christian, Ewers, Helge, Wallace, Mark I, Kukura, Philipp

“…The biological functions of the cell membrane are influenced by the mobility of its constituents, which are thought to be strongly affected by nanoscale…”
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Adhesion energy controls lipid binding-mediated endocytosis by Groza, Raluca, Schmidt, Kita Valerie, Müller, Paul Markus, Ronchi, Paolo, Schlack-Leigers, Claire, Neu, Ursula, Puchkov, Dmytro, Dimova, Rumiana, Matthaeus, Claudia, Taraska, Justin, Weikl, Thomas R., Ewers, Helge

“…Several bacterial toxins and viruses can deform membranes through multivalent binding to lipids for clathrin-independent endocytosis. However, it remains…”
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Single event visualization of unconventional secretion of FGF2 by Dimou, Eleni, Cosentino, Katia, Platonova, Evgenia, Ros, Uris, Sadeghi, Mohsen, Kashyap, Purba, Katsinelos, Taxiarchis, Wegehingel, Sabine, Noé, Frank, García-Sáez, Ana J, Ewers, Helge, Nickel, Walter

“…FGF2 is exported from cells by an unconventional secretory mechanism. Here, we directly visualized individual FGF2 membrane translocation events at the plasma…”
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Glypican-1 drives unconventional secretion of fibroblast growth factor 2 by Sparn, Carola, Dimou, Eleni, Meyer, Annalena, Saleppico, Roberto, Wegehingel, Sabine, Gerstner, Matthias, Klaus, Severina, Ewers, Helge, Nickel, Walter

“…Fibroblast growth factor 2 (FGF2) is a tumor cell survival factor that is transported into the extracellular space by an unconventional secretory mechanism…”
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Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function by Glebov, Oleg O., Jackson, Rachel E., Winterflood, Christian M., Owen, Dylan M., Barker, Ellen A., Doherty, Patrick, Ewers, Helge, Burrone, Juan

“…The active zone (AZ) matrix of presynaptic terminals coordinates the recruitment of voltage-gated calcium channels (VGCCs) and synaptic vesicles to orchestrate…”
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mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities by McEvoy, Ann L, Hoi, Hiofan, Bates, Mark, Platonova, Evgenia, Cranfill, Paula J, Baird, Michelle A, Davidson, Michael W, Ewers, Helge, Liphardt, Jan, Campbell, Robert E

“…Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible…”
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The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses by Schmerl, Bettina, Gimber, Niclas, Kuropka, Benno, Stumpf, Alexander, Rentsch, Jakob, Kunde, Stella-Amrei, von Sivers, Judith, Ewers, Helge, Schmitz, Dietmar, Freund, Christian, Schmoranzer, Jan, Rademacher, Nils, Shoichet, Sarah A

“…Recent advances in imaging technology have highlighted that scaffold proteins and receptors are arranged in subsynaptic nanodomains. The synaptic…”
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Single-Molecule Localization Microscopy using mCherry by Winterflood, Christian M., Ewers, Helge

“…We demonstrate the potential of the commonly used red fluorescent protein mCherry for single‐molecule super‐resolution imaging. mCherry can be driven into a…”
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