Search Results - "Heiter, Daniel"
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Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity From BsaXI Specificity Subunit
Published in Frontiers in microbiology (19-05-2022)“…It is stated that Bsa XI is a Type IIB restriction endonuclease (REase) that cleaves both sides of its recognition sequence 5′↓N9 AC N5 CTCC N10↓ 3′…”
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Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
Published in Frontiers in microbiology (09-04-2024)“…Winged helix (wH) domains, also termed winged helix-turn-helix (wHTH) domains, are widespread in all kingdoms of life and have diverse roles. In the context of…”
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Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases
Published in Frontiers in microbiology (18-08-2020)“…Modification dependent restriction endonucleases (MDREs) restrict modified DNA, typically with limited sequence specificity (∼2-4 bp). Here, we focus on MDREs…”
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Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI
Published in Nucleic acids research (10-01-2019)“…Abstract BbvCI, a Type IIT restriction endonuclease, recognizes and cleaves the seven base pair sequence 5′-CCTCAGC-3′, generating 3-base, 5′-overhangs. BbvCI…”
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DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target
Published in Nucleic acids research (17-02-2017)“…R.SwaI, a Type IIP restriction endonuclease, recognizes a palindromic eight base pair (bp) symmetric sequence, 5΄-ATTTAAAT-3΄, and cleaves that target at its…”
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Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family
Published in Nucleic acids research (01-12-2011)“…Novel family of putative homing endonuclease genes was recently discovered during analyses of metagenomic and genomic sequence data. One such protein is…”
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Unusual Target Site Disruption by the Rare-Cutting HNH Restriction Endonuclease PacI
Published in Structure (London) (09-06-2010)“…The crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with its eight-base-pair target recognition sequence 5′-TTAATTAA-3′ has…”
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Site-Specific DNA-nicking Mutants of the Heterodimeric Restriction Endonuclease R.BbvCI
Published in Journal of molecular biology (06-05-2005)“…The restriction enzyme R.BbvCI cleaves duplex DNA within a seven base-pair asymmetric recognition sequence, thus: CCTCAGC/GCTGAGG→CC^TCAGC/GC^TGAGG. We show…”
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A genetic dissection of the LlaJI restriction cassette reveals insights on a novel bacteriophage resistance system
Published in BMC microbiology (28-04-2006)“…Restriction/modification systems provide the dual function of protecting host DNA against restriction by methylation of appropriate bases within their…”
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Expression and Purification of Bsa XI Restriction Endonuclease and Engineering New Specificity From Bsa XI Specificity Subunit
Published in Frontiers in microbiology (2022)“…It is stated that XI is a Type IIB restriction endonuclease (REase) that cleaves both sides of its recognition sequence 5'↓N9 AC N5 CTCC N10↓ 3' (complement…”
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Cloning and characterization of the BglII restriction–modification system reveals a possible evolutionary footprint
Published in Gene (01-03-1997)“…BglII, a type II restriction–modification (R–M) system from Bacillus globigii, recognizes the sequence 5′-AGATCT-3′. The system has been cloned into E. coli in…”
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Cloning the BamHI restriction modification system
Published in Nucleic acids research (11-02-1989)“…BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have…”
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Cloning the Bam HI restriction modification system
Published in Nucleic acids research (1989)Get full text
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